Higher Resistance of <i>Yersinia enterocolitica</i> in Comparison to <i>Yersinia pseudotuberculosis</i> to Antibiotics and Cinnamon, Oregano and Thyme Essential Oils
Yersiniosis is an important zoonotic disease; however, data are scarce on the resistance of enteropathogenic yersiniae, especially that of <i>Y. pseudotuberculosis</i>. Minimum inhibitory concentrations (MIC) of 21 antibiotics and 3 essential oils (EOs) were determined by broth microdilu...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-12-01
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| Series: | Pathogens |
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| Online Access: | https://www.mdpi.com/2076-0817/11/12/1456 |
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| author | Radka Hulankova |
| author_facet | Radka Hulankova |
| author_sort | Radka Hulankova |
| collection | DOAJ |
| description | Yersiniosis is an important zoonotic disease; however, data are scarce on the resistance of enteropathogenic yersiniae, especially that of <i>Y. pseudotuberculosis</i>. Minimum inhibitory concentrations (MIC) of 21 antibiotics and 3 essential oils (EOs) were determined by broth microdilution for <i>Y. enterocolitica</i> bioserotype 4/O:3 strains isolated from domestic swine (<i>n</i> = 132) and <i>Y. pseudotuberculosis</i> strains isolated from wild boars (<i>n</i> = 46). For 15 of 21 antibiotics, statistically significant differences were found between MIC values of <i>Y. enterocolitica</i> and <i>Y. pseudotuberculosis</i>. While <i>Y. enterocolitica</i> was more resistant to amoxiclav, ampicillin, cefotaxime, cefuroxime, gentamicin, imipenem, meropenem, tetracycline, tobramycin, and trimethoprim, <i>Y. pseudotuberculosis</i> was more resistant to cefepime, ceftazidime, colistin, erythromycin, and nitrofurantoin. Statistically significant differences were found between various essential oils (<i>p</i> < 0.001) and species (<i>p</i> < 0.001). The lowest MICs for multiresistant <i>Y. enterocolitica</i> (<i>n</i> = 12) and <i>Y. pseudotuberculosis</i> (<i>n</i> = 12) were obtained for cinnamon (median 414 and 207 μg/mL, respectively) and oregano EOs (median 379 and 284 μg/mL), whereas thyme EO showed significantly higher MIC values (median 738 and 553 μg/mL; <i>p</i> < 0.001). There was no difference between <i>Y. enterocolitica</i> strains of plant (1A) and animal (4/O:3) origin (<i>p</i> = 0.855). The results show that <i>Y. enterocolitica</i> is generally more resistant to antimicrobials than <i>Y. pseudotuberculosis</i>. |
| first_indexed | 2024-03-09T15:59:47Z |
| format | Article |
| id | doaj.art-e3cbd1565c0f4a2c90c9429d4d27692d |
| institution | Directory Open Access Journal |
| issn | 2076-0817 |
| language | English |
| last_indexed | 2024-03-09T15:59:47Z |
| publishDate | 2022-12-01 |
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| spelling | doaj.art-e3cbd1565c0f4a2c90c9429d4d27692d2023-11-24T17:13:02ZengMDPI AGPathogens2076-08172022-12-011112145610.3390/pathogens11121456Higher Resistance of <i>Yersinia enterocolitica</i> in Comparison to <i>Yersinia pseudotuberculosis</i> to Antibiotics and Cinnamon, Oregano and Thyme Essential OilsRadka Hulankova0Department of Animal Origin Food and Gastronomic Sciences, Faculty of Veterinary Hygiene and Ecology, University of Veterinary Sciences Brno, 612 42 Brno, Czech RepublicYersiniosis is an important zoonotic disease; however, data are scarce on the resistance of enteropathogenic yersiniae, especially that of <i>Y. pseudotuberculosis</i>. Minimum inhibitory concentrations (MIC) of 21 antibiotics and 3 essential oils (EOs) were determined by broth microdilution for <i>Y. enterocolitica</i> bioserotype 4/O:3 strains isolated from domestic swine (<i>n</i> = 132) and <i>Y. pseudotuberculosis</i> strains isolated from wild boars (<i>n</i> = 46). For 15 of 21 antibiotics, statistically significant differences were found between MIC values of <i>Y. enterocolitica</i> and <i>Y. pseudotuberculosis</i>. While <i>Y. enterocolitica</i> was more resistant to amoxiclav, ampicillin, cefotaxime, cefuroxime, gentamicin, imipenem, meropenem, tetracycline, tobramycin, and trimethoprim, <i>Y. pseudotuberculosis</i> was more resistant to cefepime, ceftazidime, colistin, erythromycin, and nitrofurantoin. Statistically significant differences were found between various essential oils (<i>p</i> < 0.001) and species (<i>p</i> < 0.001). The lowest MICs for multiresistant <i>Y. enterocolitica</i> (<i>n</i> = 12) and <i>Y. pseudotuberculosis</i> (<i>n</i> = 12) were obtained for cinnamon (median 414 and 207 μg/mL, respectively) and oregano EOs (median 379 and 284 μg/mL), whereas thyme EO showed significantly higher MIC values (median 738 and 553 μg/mL; <i>p</i> < 0.001). There was no difference between <i>Y. enterocolitica</i> strains of plant (1A) and animal (4/O:3) origin (<i>p</i> = 0.855). The results show that <i>Y. enterocolitica</i> is generally more resistant to antimicrobials than <i>Y. pseudotuberculosis</i>.https://www.mdpi.com/2076-0817/11/12/1456minimum inhibitory concentrationantibiotic resistancebroth microdilutionantimicrobialsmultiresistancesusceptibility |
| spellingShingle | Radka Hulankova Higher Resistance of <i>Yersinia enterocolitica</i> in Comparison to <i>Yersinia pseudotuberculosis</i> to Antibiotics and Cinnamon, Oregano and Thyme Essential Oils Pathogens minimum inhibitory concentration antibiotic resistance broth microdilution antimicrobials multiresistance susceptibility |
| title | Higher Resistance of <i>Yersinia enterocolitica</i> in Comparison to <i>Yersinia pseudotuberculosis</i> to Antibiotics and Cinnamon, Oregano and Thyme Essential Oils |
| title_full | Higher Resistance of <i>Yersinia enterocolitica</i> in Comparison to <i>Yersinia pseudotuberculosis</i> to Antibiotics and Cinnamon, Oregano and Thyme Essential Oils |
| title_fullStr | Higher Resistance of <i>Yersinia enterocolitica</i> in Comparison to <i>Yersinia pseudotuberculosis</i> to Antibiotics and Cinnamon, Oregano and Thyme Essential Oils |
| title_full_unstemmed | Higher Resistance of <i>Yersinia enterocolitica</i> in Comparison to <i>Yersinia pseudotuberculosis</i> to Antibiotics and Cinnamon, Oregano and Thyme Essential Oils |
| title_short | Higher Resistance of <i>Yersinia enterocolitica</i> in Comparison to <i>Yersinia pseudotuberculosis</i> to Antibiotics and Cinnamon, Oregano and Thyme Essential Oils |
| title_sort | higher resistance of i yersinia enterocolitica i in comparison to i yersinia pseudotuberculosis i to antibiotics and cinnamon oregano and thyme essential oils |
| topic | minimum inhibitory concentration antibiotic resistance broth microdilution antimicrobials multiresistance susceptibility |
| url | https://www.mdpi.com/2076-0817/11/12/1456 |
| work_keys_str_mv | AT radkahulankova higherresistanceofiyersiniaenterocoliticaiincomparisontoiyersiniapseudotuberculosisitoantibioticsandcinnamonoreganoandthymeessentialoils |