Characterization of serum biomarkers and antibody responses against Prevotella spp. in preclinical and new-onset phase of rheumatic diseases
IntroductionThe characterization of the influence of the microbiota on the development and drug responses during rheumatic diseases has intensified in recent years. The role of specific bacteria during disease development has become a central research question. Notably, several lines of evidence poi...
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Frontiers Media S.A.
2023-01-01
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| Series: | Frontiers in Cellular and Infection Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fcimb.2022.1096211/full |
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| author | Lena Amend Benoît Thomas P. Gilbert Penelope Pelczar Marius Böttcher Samuel Huber Torsten Witte Axel Finckh Till Strowig |
| author_facet | Lena Amend Benoît Thomas P. Gilbert Penelope Pelczar Marius Böttcher Samuel Huber Torsten Witte Axel Finckh Till Strowig |
| author_sort | Lena Amend |
| collection | DOAJ |
| description | IntroductionThe characterization of the influence of the microbiota on the development and drug responses during rheumatic diseases has intensified in recent years. The role of specific bacteria during disease development has become a central research question. Notably, several lines of evidence point to distinct microbes, e.g., Prevotella copri (P. copri) being targeted by antibodies in clinical phases of rheumatic diseases.MethodsIn the present study, we compiled a broad collection of human serum samples from individuals at risk of developing RA, chronic RA patients as well as patients with new-onset of rheumatic diseases. We evaluated the presence of inflammatory biomarkers in our serum collection as well as serum antibody responses against novel, genetically distinct isolates of P. copri and several oral pathobionts.ResultsOur analysis revealed the presence of increased levels of inflammatory markers already in pre-clinical and new onset rheumatoid arthritis. However, antibody reactivity against the microbes did not differ between patient groups. Yet, we observed high variability between the different P. copri strains. We found total serum IgG levels to slightly correlate with IgG antibody responses against P. copri, but no relation between the latter and presence or prevalence of P. copri in the intestine.DiscussionIn conclusion, our work underlined the importance of strain-level characterization and its consideration during further investigations of host-microbiota interactions and the development of microbiome-based therapeutic approaches for treating rheumatic diseases. |
| first_indexed | 2024-04-10T22:19:14Z |
| format | Article |
| id | doaj.art-e3cd79bc37b04051bb988bdc08a0f687 |
| institution | Directory Open Access Journal |
| issn | 2235-2988 |
| language | English |
| last_indexed | 2024-04-10T22:19:14Z |
| publishDate | 2023-01-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Cellular and Infection Microbiology |
| spelling | doaj.art-e3cd79bc37b04051bb988bdc08a0f6872023-01-18T05:38:06ZengFrontiers Media S.A.Frontiers in Cellular and Infection Microbiology2235-29882023-01-011210.3389/fcimb.2022.10962111096211Characterization of serum biomarkers and antibody responses against Prevotella spp. in preclinical and new-onset phase of rheumatic diseasesLena Amend0Benoît Thomas P. Gilbert1Penelope Pelczar2Marius Böttcher3Samuel Huber4Torsten Witte5Axel Finckh6Till Strowig7Department of Microbial Immune Regulation, Helmholtz Centre for Infection Research, Braunschweig, GermanyDivision of Rheumatology, Geneva University Hospitals, Geneva, SwitzerlandI. Department of Medicine, University Medical Center Hamburg-Eppendorf, Hamburg, GermanyI. Department of Medicine, University Medical Center Hamburg-Eppendorf, Hamburg, GermanyI. Department of Medicine, University Medical Center Hamburg-Eppendorf, Hamburg, GermanyDepartment of Rheumatology and Immunology, Hannover Medical School, Hannover, GermanyDivision of Rheumatology, Geneva University Hospitals, Geneva, SwitzerlandDepartment of Microbial Immune Regulation, Helmholtz Centre for Infection Research, Braunschweig, GermanyIntroductionThe characterization of the influence of the microbiota on the development and drug responses during rheumatic diseases has intensified in recent years. The role of specific bacteria during disease development has become a central research question. Notably, several lines of evidence point to distinct microbes, e.g., Prevotella copri (P. copri) being targeted by antibodies in clinical phases of rheumatic diseases.MethodsIn the present study, we compiled a broad collection of human serum samples from individuals at risk of developing RA, chronic RA patients as well as patients with new-onset of rheumatic diseases. We evaluated the presence of inflammatory biomarkers in our serum collection as well as serum antibody responses against novel, genetically distinct isolates of P. copri and several oral pathobionts.ResultsOur analysis revealed the presence of increased levels of inflammatory markers already in pre-clinical and new onset rheumatoid arthritis. However, antibody reactivity against the microbes did not differ between patient groups. Yet, we observed high variability between the different P. copri strains. We found total serum IgG levels to slightly correlate with IgG antibody responses against P. copri, but no relation between the latter and presence or prevalence of P. copri in the intestine.DiscussionIn conclusion, our work underlined the importance of strain-level characterization and its consideration during further investigations of host-microbiota interactions and the development of microbiome-based therapeutic approaches for treating rheumatic diseases.https://www.frontiersin.org/articles/10.3389/fcimb.2022.1096211/fullgut microbiotaPrevotella coprispondyloarthritisrheumatoid arthritispreclinical disease stagesbiomarkers |
| spellingShingle | Lena Amend Benoît Thomas P. Gilbert Penelope Pelczar Marius Böttcher Samuel Huber Torsten Witte Axel Finckh Till Strowig Characterization of serum biomarkers and antibody responses against Prevotella spp. in preclinical and new-onset phase of rheumatic diseases Frontiers in Cellular and Infection Microbiology gut microbiota Prevotella copri spondyloarthritis rheumatoid arthritis preclinical disease stages biomarkers |
| title | Characterization of serum biomarkers and antibody responses against Prevotella spp. in preclinical and new-onset phase of rheumatic diseases |
| title_full | Characterization of serum biomarkers and antibody responses against Prevotella spp. in preclinical and new-onset phase of rheumatic diseases |
| title_fullStr | Characterization of serum biomarkers and antibody responses against Prevotella spp. in preclinical and new-onset phase of rheumatic diseases |
| title_full_unstemmed | Characterization of serum biomarkers and antibody responses against Prevotella spp. in preclinical and new-onset phase of rheumatic diseases |
| title_short | Characterization of serum biomarkers and antibody responses against Prevotella spp. in preclinical and new-onset phase of rheumatic diseases |
| title_sort | characterization of serum biomarkers and antibody responses against prevotella spp in preclinical and new onset phase of rheumatic diseases |
| topic | gut microbiota Prevotella copri spondyloarthritis rheumatoid arthritis preclinical disease stages biomarkers |
| url | https://www.frontiersin.org/articles/10.3389/fcimb.2022.1096211/full |
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