A proof-of-concept study on endoscopic ultrasound-guided biopsy of detrusor muscle in porcine bladders
IntroductionConventionally, we rely on transurethral resection of bladder tumour (TURBT) for local staging of muscle-invasive bladder cancer (MIBC). However, the procedure is limited by its staging inaccuracy which may delay the definitive treatment of MIBC.MethodsWe conducted a proof-of concept stu...
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| Format: | Article |
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Frontiers Media S.A.
2023-06-01
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| Series: | Frontiers in Oncology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fonc.2023.1160463/full |
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| author | Jeremy Yuen-Chun Teoh Jeremy Yuen-Chun Teoh Chak-Lam Cho Ronald Cheong-Kin Chan Kang Liu Hongda Zhao Gianluca Giannarini Dmitry Enikeev Dmitry Enikeev Chi-Fai Ng Anthony Yuen-Bun Teoh |
| author_facet | Jeremy Yuen-Chun Teoh Jeremy Yuen-Chun Teoh Chak-Lam Cho Ronald Cheong-Kin Chan Kang Liu Hongda Zhao Gianluca Giannarini Dmitry Enikeev Dmitry Enikeev Chi-Fai Ng Anthony Yuen-Bun Teoh |
| author_sort | Jeremy Yuen-Chun Teoh |
| collection | DOAJ |
| description | IntroductionConventionally, we rely on transurethral resection of bladder tumour (TURBT) for local staging of muscle-invasive bladder cancer (MIBC). However, the procedure is limited by its staging inaccuracy which may delay the definitive treatment of MIBC.MethodsWe conducted a proof-of concept study on endoscopic ultrasound (EUS)-guided biopsy of detrusor muscle in porcine bladders. Five porcine bladders were used in this experiment. Upon EUS, four layers of tissue including the mucosa (hypoechoic), submucosa (hyperechoic), detrusor muscle (hypoechoic) and serosa (hyperechoic) could be identified.ResultsA total of 37 EUS-guided biopsies were taken from 15 sites (three sites per bladder), and the mean number of biopsies taken from each site was 2.47±0.64. Among the 37 biopsies, 30 of them (81.1%) obtained detrusor muscle in the biopsy specimen. For the per biopsy site analysis, detrusor muscle was obtained in 73.3% if only one biopsy was taken, and 100% if two or more biopsies were taken from the same biopsy site. Overall, detrusor muscle was successfully obtained from all 15 biopsy sites (100%). No bladder perforation was observed throughout all biopsy processes.ConclusionEUS-guided biopsy of the detrusor muscle could be performed during the initial cystoscopy session, thus expediting the histological diagnosis and subsequent treatment of MIBC. |
| first_indexed | 2024-03-13T07:55:34Z |
| format | Article |
| id | doaj.art-e3d1f50d1ecb423ea18aea621bfb4abf |
| institution | Directory Open Access Journal |
| issn | 2234-943X |
| language | English |
| last_indexed | 2024-03-13T07:55:34Z |
| publishDate | 2023-06-01 |
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| record_format | Article |
| series | Frontiers in Oncology |
| spelling | doaj.art-e3d1f50d1ecb423ea18aea621bfb4abf2023-06-02T06:02:38ZengFrontiers Media S.A.Frontiers in Oncology2234-943X2023-06-011310.3389/fonc.2023.11604631160463A proof-of-concept study on endoscopic ultrasound-guided biopsy of detrusor muscle in porcine bladdersJeremy Yuen-Chun Teoh0Jeremy Yuen-Chun Teoh1Chak-Lam Cho2Ronald Cheong-Kin Chan3Kang Liu4Hongda Zhao5Gianluca Giannarini6Dmitry Enikeev7Dmitry Enikeev8Chi-Fai Ng9Anthony Yuen-Bun Teoh10S.H. Ho Urology Centre, Department of Surgery, The Chinese University of Hong Kong, Hong Kong, Hong Kong SAR, ChinaUrothelial Carcinoma Working Group, European Association of Urology – Young Academic Urologists (EAU-YAU), Arnhem, NetherlandsS.H. Ho Urology Centre, Department of Surgery, The Chinese University of Hong Kong, Hong Kong, Hong Kong SAR, ChinaDepartment of Anatomical and Cellular Pathology, The Chinese University of Hong Kong, Hong Kong, Hong Kong SAR, ChinaS.H. Ho Urology Centre, Department of Surgery, The Chinese University of Hong Kong, Hong Kong, Hong Kong SAR, ChinaS.H. Ho Urology Centre, Department of Surgery, The Chinese University of Hong Kong, Hong Kong, Hong Kong SAR, ChinaUrology Unit, “Santa Maria della Misericordia” University Hospital, Udine, ItalyInstitute for Urology and Reproductive Health, Sechenov University, Moscow, RussiaDepartment of Urology, Medical University of Vienna, Vienna, AustriaS.H. Ho Urology Centre, Department of Surgery, The Chinese University of Hong Kong, Hong Kong, Hong Kong SAR, ChinaDepartment of Surgery, Prince of Wales Hospital, The Chinese University of Hong Kong, Hong Kong, Hong Kong SAR, ChinaIntroductionConventionally, we rely on transurethral resection of bladder tumour (TURBT) for local staging of muscle-invasive bladder cancer (MIBC). However, the procedure is limited by its staging inaccuracy which may delay the definitive treatment of MIBC.MethodsWe conducted a proof-of concept study on endoscopic ultrasound (EUS)-guided biopsy of detrusor muscle in porcine bladders. Five porcine bladders were used in this experiment. Upon EUS, four layers of tissue including the mucosa (hypoechoic), submucosa (hyperechoic), detrusor muscle (hypoechoic) and serosa (hyperechoic) could be identified.ResultsA total of 37 EUS-guided biopsies were taken from 15 sites (three sites per bladder), and the mean number of biopsies taken from each site was 2.47±0.64. Among the 37 biopsies, 30 of them (81.1%) obtained detrusor muscle in the biopsy specimen. For the per biopsy site analysis, detrusor muscle was obtained in 73.3% if only one biopsy was taken, and 100% if two or more biopsies were taken from the same biopsy site. Overall, detrusor muscle was successfully obtained from all 15 biopsy sites (100%). No bladder perforation was observed throughout all biopsy processes.ConclusionEUS-guided biopsy of the detrusor muscle could be performed during the initial cystoscopy session, thus expediting the histological diagnosis and subsequent treatment of MIBC.https://www.frontiersin.org/articles/10.3389/fonc.2023.1160463/fulldetrusor muscleendoscopic ultrasound-guided biopsyporcine bladdertransurethral resection of bladder tumor (TURBT)diagnosis |
| spellingShingle | Jeremy Yuen-Chun Teoh Jeremy Yuen-Chun Teoh Chak-Lam Cho Ronald Cheong-Kin Chan Kang Liu Hongda Zhao Gianluca Giannarini Dmitry Enikeev Dmitry Enikeev Chi-Fai Ng Anthony Yuen-Bun Teoh A proof-of-concept study on endoscopic ultrasound-guided biopsy of detrusor muscle in porcine bladders Frontiers in Oncology detrusor muscle endoscopic ultrasound-guided biopsy porcine bladder transurethral resection of bladder tumor (TURBT) diagnosis |
| title | A proof-of-concept study on endoscopic ultrasound-guided biopsy of detrusor muscle in porcine bladders |
| title_full | A proof-of-concept study on endoscopic ultrasound-guided biopsy of detrusor muscle in porcine bladders |
| title_fullStr | A proof-of-concept study on endoscopic ultrasound-guided biopsy of detrusor muscle in porcine bladders |
| title_full_unstemmed | A proof-of-concept study on endoscopic ultrasound-guided biopsy of detrusor muscle in porcine bladders |
| title_short | A proof-of-concept study on endoscopic ultrasound-guided biopsy of detrusor muscle in porcine bladders |
| title_sort | proof of concept study on endoscopic ultrasound guided biopsy of detrusor muscle in porcine bladders |
| topic | detrusor muscle endoscopic ultrasound-guided biopsy porcine bladder transurethral resection of bladder tumor (TURBT) diagnosis |
| url | https://www.frontiersin.org/articles/10.3389/fonc.2023.1160463/full |
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