Generation of imidazolinone herbicide resistant trait in Arabidopsis.
Recently-emerged base editing technologies could create single base mutations at precise genomic positions without generation DNA double strand breaks. Herbicide resistant mutations have been successfully introduced to different plant species, including Arabidopsis, watermelon, wheat, potato and tom...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2020-01-01
|
| Series: | PLoS ONE |
| Online Access: | https://doi.org/10.1371/journal.pone.0233503 |
| _version_ | 1818583985426530304 |
|---|---|
| author | Huirong Dong Delin Wang Zhijing Bai Yuge Yuan Wei Yang Yuexia Zhang Hanwen Ni Linjian Jiang |
| author_facet | Huirong Dong Delin Wang Zhijing Bai Yuge Yuan Wei Yang Yuexia Zhang Hanwen Ni Linjian Jiang |
| author_sort | Huirong Dong |
| collection | DOAJ |
| description | Recently-emerged base editing technologies could create single base mutations at precise genomic positions without generation DNA double strand breaks. Herbicide resistant mutations have been successfully introduced to different plant species, including Arabidopsis, watermelon, wheat, potato and tomato via C to T (or G to A on the complementary strand) base editors (CBE) at the P197 position of endogenous acetolactate synthase (ALS) genes. Additionally, G to A conversion to another conserved amino acid S653 on ALS gene could confer tolerance to imidazolinone herbicides. However, no such mutation was successfully generated via CBE, likely due to the target C base is outside of the classic base editing window. Since CBE driven by egg cell (EC) specific promoter would re-edit the wild type alleles in egg cells and early embryos, we hypothesized the diversity of base editing outcomes could be largely increased at later generations to allow selection of desired herbicide resistant mutants. To test this hypothesis, we aimed to introduce C to T conversion to the complement strand of S653 codon at ALS gene, hosting a C at the 10th position within the 20-nt spacer sequence outside of the classic base editing window. While we did not detect base-edited T1 plants, efficient and diverse base edits emerged at later generations. Herbicide resistant mutants with different editing outcomes were recovered when T3 and T4 seeds were subject to herbicide selection. As expected, most herbicide resistant plants contained S653N mutation as a result of G10 to A10. Our results showed that CBE could create imidazolinone herbicide resistant trait in Arabidopsis and be potentially applied to crops to facilitate weed control. |
| first_indexed | 2024-12-16T08:13:59Z |
| format | Article |
| id | doaj.art-e3d22b525f104b90ba519d31b2fe811a |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-16T08:13:59Z |
| publishDate | 2020-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-e3d22b525f104b90ba519d31b2fe811a2022-12-21T22:38:19ZengPublic Library of Science (PLoS)PLoS ONE1932-62032020-01-01155e023350310.1371/journal.pone.0233503Generation of imidazolinone herbicide resistant trait in Arabidopsis.Huirong DongDelin WangZhijing BaiYuge YuanWei YangYuexia ZhangHanwen NiLinjian JiangRecently-emerged base editing technologies could create single base mutations at precise genomic positions without generation DNA double strand breaks. Herbicide resistant mutations have been successfully introduced to different plant species, including Arabidopsis, watermelon, wheat, potato and tomato via C to T (or G to A on the complementary strand) base editors (CBE) at the P197 position of endogenous acetolactate synthase (ALS) genes. Additionally, G to A conversion to another conserved amino acid S653 on ALS gene could confer tolerance to imidazolinone herbicides. However, no such mutation was successfully generated via CBE, likely due to the target C base is outside of the classic base editing window. Since CBE driven by egg cell (EC) specific promoter would re-edit the wild type alleles in egg cells and early embryos, we hypothesized the diversity of base editing outcomes could be largely increased at later generations to allow selection of desired herbicide resistant mutants. To test this hypothesis, we aimed to introduce C to T conversion to the complement strand of S653 codon at ALS gene, hosting a C at the 10th position within the 20-nt spacer sequence outside of the classic base editing window. While we did not detect base-edited T1 plants, efficient and diverse base edits emerged at later generations. Herbicide resistant mutants with different editing outcomes were recovered when T3 and T4 seeds were subject to herbicide selection. As expected, most herbicide resistant plants contained S653N mutation as a result of G10 to A10. Our results showed that CBE could create imidazolinone herbicide resistant trait in Arabidopsis and be potentially applied to crops to facilitate weed control.https://doi.org/10.1371/journal.pone.0233503 |
| spellingShingle | Huirong Dong Delin Wang Zhijing Bai Yuge Yuan Wei Yang Yuexia Zhang Hanwen Ni Linjian Jiang Generation of imidazolinone herbicide resistant trait in Arabidopsis. PLoS ONE |
| title | Generation of imidazolinone herbicide resistant trait in Arabidopsis. |
| title_full | Generation of imidazolinone herbicide resistant trait in Arabidopsis. |
| title_fullStr | Generation of imidazolinone herbicide resistant trait in Arabidopsis. |
| title_full_unstemmed | Generation of imidazolinone herbicide resistant trait in Arabidopsis. |
| title_short | Generation of imidazolinone herbicide resistant trait in Arabidopsis. |
| title_sort | generation of imidazolinone herbicide resistant trait in arabidopsis |
| url | https://doi.org/10.1371/journal.pone.0233503 |
| work_keys_str_mv | AT huirongdong generationofimidazolinoneherbicideresistanttraitinarabidopsis AT delinwang generationofimidazolinoneherbicideresistanttraitinarabidopsis AT zhijingbai generationofimidazolinoneherbicideresistanttraitinarabidopsis AT yugeyuan generationofimidazolinoneherbicideresistanttraitinarabidopsis AT weiyang generationofimidazolinoneherbicideresistanttraitinarabidopsis AT yuexiazhang generationofimidazolinoneherbicideresistanttraitinarabidopsis AT hanwenni generationofimidazolinoneherbicideresistanttraitinarabidopsis AT linjianjiang generationofimidazolinoneherbicideresistanttraitinarabidopsis |