Double Immunochromatographic Test System for Sensitive Detection of Phycotoxins Domoic Acid and Okadaic Acid in Seawater and Seafood
In this investigation, a double immunochromatographic analysis (ICA) of two relevant phycotoxins, domoic acid (DA) and okadaic acid (OA), was developed for the first time. The ICA was performed in the indirect competitive format using gold nanoparticles conjugated with anti-species antibodies. Under...
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MDPI AG
2022-09-01
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| Series: | Micromachines |
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| Online Access: | https://www.mdpi.com/2072-666X/13/9/1506 |
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| author | Olga D. Hendrickson Elena A. Zvereva Olga N. Solopova Anatoly V. Zherdev Peter G. Sveshnikov Sergei A. Eremin Boris B. Dzantiev |
| author_facet | Olga D. Hendrickson Elena A. Zvereva Olga N. Solopova Anatoly V. Zherdev Peter G. Sveshnikov Sergei A. Eremin Boris B. Dzantiev |
| author_sort | Olga D. Hendrickson |
| collection | DOAJ |
| description | In this investigation, a double immunochromatographic analysis (ICA) of two relevant phycotoxins, domoic acid (DA) and okadaic acid (OA), was developed for the first time. The ICA was performed in the indirect competitive format using gold nanoparticles conjugated with anti-species antibodies. Under optimal conditions, the instrumental detection limits/cutoffs for simultaneous detection of DA and OA were 1.2/100 and 0.1/2.5 ng/mL, respectively. The time of the assay was 18 min. The ICA was applied to test seawater and a large panel of seafood, including mussels, tiger shrimps, octopuses, whelks, crabs, and scallops. The proposed simple sample preparation method for seafood takes only 20 min. For seawater, a dilution by buffer was implemented. The assay recoveries varied from 80.8% to 124.5%. The competitive potential of the proposed technique as a tool to control natural water and seafood samples is determined by its simplicity, rapidity, and sensitivity. |
| first_indexed | 2024-03-09T23:08:18Z |
| format | Article |
| id | doaj.art-e3d5ab82cb584c88b5fb302dfa75ff0a |
| institution | Directory Open Access Journal |
| issn | 2072-666X |
| language | English |
| last_indexed | 2024-03-09T23:08:18Z |
| publishDate | 2022-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Micromachines |
| spelling | doaj.art-e3d5ab82cb584c88b5fb302dfa75ff0a2023-11-23T17:50:39ZengMDPI AGMicromachines2072-666X2022-09-01139150610.3390/mi13091506Double Immunochromatographic Test System for Sensitive Detection of Phycotoxins Domoic Acid and Okadaic Acid in Seawater and SeafoodOlga D. Hendrickson0Elena A. Zvereva1Olga N. Solopova2Anatoly V. Zherdev3Peter G. Sveshnikov4Sergei A. Eremin5Boris B. Dzantiev6Bach Institute of Biochemistry, Research Center of Biotechnology, Russian Academy of Sciences, Leninsky Prospect 33, 119071 Moscow, RussiaBach Institute of Biochemistry, Research Center of Biotechnology, Russian Academy of Sciences, Leninsky Prospect 33, 119071 Moscow, RussiaBlokhin National Medical Research Center of Oncology, Ministry of Health of the Russian Federation, Kashirskoye Shosse 24, 115478 Moscow, RussiaBach Institute of Biochemistry, Research Center of Biotechnology, Russian Academy of Sciences, Leninsky Prospect 33, 119071 Moscow, RussiaRussian Research Center for Molecular Diagnostics and Therapy, Sympheropolsky Blvrd., 8, 117638 Moscow, RussiaBach Institute of Biochemistry, Research Center of Biotechnology, Russian Academy of Sciences, Leninsky Prospect 33, 119071 Moscow, RussiaBach Institute of Biochemistry, Research Center of Biotechnology, Russian Academy of Sciences, Leninsky Prospect 33, 119071 Moscow, RussiaIn this investigation, a double immunochromatographic analysis (ICA) of two relevant phycotoxins, domoic acid (DA) and okadaic acid (OA), was developed for the first time. The ICA was performed in the indirect competitive format using gold nanoparticles conjugated with anti-species antibodies. Under optimal conditions, the instrumental detection limits/cutoffs for simultaneous detection of DA and OA were 1.2/100 and 0.1/2.5 ng/mL, respectively. The time of the assay was 18 min. The ICA was applied to test seawater and a large panel of seafood, including mussels, tiger shrimps, octopuses, whelks, crabs, and scallops. The proposed simple sample preparation method for seafood takes only 20 min. For seawater, a dilution by buffer was implemented. The assay recoveries varied from 80.8% to 124.5%. The competitive potential of the proposed technique as a tool to control natural water and seafood samples is determined by its simplicity, rapidity, and sensitivity.https://www.mdpi.com/2072-666X/13/9/1506marine toxinsimmunochromatographic analysisseafoodseawaterfood security |
| spellingShingle | Olga D. Hendrickson Elena A. Zvereva Olga N. Solopova Anatoly V. Zherdev Peter G. Sveshnikov Sergei A. Eremin Boris B. Dzantiev Double Immunochromatographic Test System for Sensitive Detection of Phycotoxins Domoic Acid and Okadaic Acid in Seawater and Seafood Micromachines marine toxins immunochromatographic analysis seafood seawater food security |
| title | Double Immunochromatographic Test System for Sensitive Detection of Phycotoxins Domoic Acid and Okadaic Acid in Seawater and Seafood |
| title_full | Double Immunochromatographic Test System for Sensitive Detection of Phycotoxins Domoic Acid and Okadaic Acid in Seawater and Seafood |
| title_fullStr | Double Immunochromatographic Test System for Sensitive Detection of Phycotoxins Domoic Acid and Okadaic Acid in Seawater and Seafood |
| title_full_unstemmed | Double Immunochromatographic Test System for Sensitive Detection of Phycotoxins Domoic Acid and Okadaic Acid in Seawater and Seafood |
| title_short | Double Immunochromatographic Test System for Sensitive Detection of Phycotoxins Domoic Acid and Okadaic Acid in Seawater and Seafood |
| title_sort | double immunochromatographic test system for sensitive detection of phycotoxins domoic acid and okadaic acid in seawater and seafood |
| topic | marine toxins immunochromatographic analysis seafood seawater food security |
| url | https://www.mdpi.com/2072-666X/13/9/1506 |
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