Biodecolorization of textile azo dye using Bacillus sp. strain CH12 isolated from alkaline lake
Textile azo dye decolorizing bacteria were isolated from alkaline Lakes Abaya and Chamo using Reactive Red 239 (RR239) dye. Through subsequent screening process, strain CH12 was selected to investigate the effects of nutrient supplement, DO, pH, temperature, dye concentration and types on decoloriza...
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| Format: | Article |
| Language: | English |
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Elsevier
2017-09-01
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| Series: | Biotechnology Reports |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2215017X1630056X |
| _version_ | 1818922399468355584 |
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| author | Awoke Guadie Samson Tizazu Meseretu Melese Wenshan Guo Huu Hao Ngo Siqing Xia |
| author_facet | Awoke Guadie Samson Tizazu Meseretu Melese Wenshan Guo Huu Hao Ngo Siqing Xia |
| author_sort | Awoke Guadie |
| collection | DOAJ |
| description | Textile azo dye decolorizing bacteria were isolated from alkaline Lakes Abaya and Chamo using Reactive Red 239 (RR239) dye. Through subsequent screening process, strain CH12 was selected to investigate the effects of nutrient supplement, DO, pH, temperature, dye concentration and types on decolorization. Based on 16S rRNA gene sequence analysis, strain CH12 was identified as Bacillus sp. Decolorization efficiencies were significantly enhanced with carbon (≥98%) and organic nitrogen (∼100%) supplements. Complete decolorization was also observed under anoxic and anaerobic conditions, and at the temperature of 30 °C and the pH of 10. However, the azo dye decolorization efficiency of strain CH12 was significantly reduced when NaNO3 (1–8%) was supplemented or under aerobic culturing condition (≤6%), indicating that RR239 was less preferred electron acceptor. Overall, strain CH12 can be a promising candidate for decolorization applications due to its potential to effectively decolorize higher RR239 concentrations (50−250 mg/L) and six additional dyes. |
| first_indexed | 2024-12-20T01:52:55Z |
| format | Article |
| id | doaj.art-e3d86f23bbb84d13864bf5a017f56717 |
| institution | Directory Open Access Journal |
| issn | 2215-017X |
| language | English |
| last_indexed | 2024-12-20T01:52:55Z |
| publishDate | 2017-09-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Biotechnology Reports |
| spelling | doaj.art-e3d86f23bbb84d13864bf5a017f567172022-12-21T19:57:35ZengElsevierBiotechnology Reports2215-017X2017-09-0115C9210010.1016/j.btre.2017.06.007Biodecolorization of textile azo dye using Bacillus sp. strain CH12 isolated from alkaline lakeAwoke Guadie0Samson Tizazu1Meseretu Melese2Wenshan Guo3Huu Hao Ngo4Siqing Xia5College of Natural Sciences, Arba Minch University, Arba Minch, EthiopiaCollege of Natural Sciences, Arba Minch University, Arba Minch, EthiopiaBiological and Cultural Diversity Research Center, Arba Minch, EthiopiaCentre for Technology in Water and Wastewater, School of Civil and Environmental, Engineering, University of Technology Sydney, Sydney, NWS 2007, AustraliaCentre for Technology in Water and Wastewater, School of Civil and Environmental, Engineering, University of Technology Sydney, Sydney, NWS 2007, AustraliaState Key Laboratory of Pollution Control and Resource Reuse, College of Environmental, Science and Engineering, Tongji University, Shanghai, ChinaTextile azo dye decolorizing bacteria were isolated from alkaline Lakes Abaya and Chamo using Reactive Red 239 (RR239) dye. Through subsequent screening process, strain CH12 was selected to investigate the effects of nutrient supplement, DO, pH, temperature, dye concentration and types on decolorization. Based on 16S rRNA gene sequence analysis, strain CH12 was identified as Bacillus sp. Decolorization efficiencies were significantly enhanced with carbon (≥98%) and organic nitrogen (∼100%) supplements. Complete decolorization was also observed under anoxic and anaerobic conditions, and at the temperature of 30 °C and the pH of 10. However, the azo dye decolorization efficiency of strain CH12 was significantly reduced when NaNO3 (1–8%) was supplemented or under aerobic culturing condition (≤6%), indicating that RR239 was less preferred electron acceptor. Overall, strain CH12 can be a promising candidate for decolorization applications due to its potential to effectively decolorize higher RR239 concentrations (50−250 mg/L) and six additional dyes.http://www.sciencedirect.com/science/article/pii/S2215017X1630056XAlkaline lakeBacillus sp.BiodecolorizationStrain CH12Reactive Red 329 |
| spellingShingle | Awoke Guadie Samson Tizazu Meseretu Melese Wenshan Guo Huu Hao Ngo Siqing Xia Biodecolorization of textile azo dye using Bacillus sp. strain CH12 isolated from alkaline lake Biotechnology Reports Alkaline lake Bacillus sp. Biodecolorization Strain CH12 Reactive Red 329 |
| title | Biodecolorization of textile azo dye using Bacillus sp. strain CH12 isolated from alkaline lake |
| title_full | Biodecolorization of textile azo dye using Bacillus sp. strain CH12 isolated from alkaline lake |
| title_fullStr | Biodecolorization of textile azo dye using Bacillus sp. strain CH12 isolated from alkaline lake |
| title_full_unstemmed | Biodecolorization of textile azo dye using Bacillus sp. strain CH12 isolated from alkaline lake |
| title_short | Biodecolorization of textile azo dye using Bacillus sp. strain CH12 isolated from alkaline lake |
| title_sort | biodecolorization of textile azo dye using bacillus sp strain ch12 isolated from alkaline lake |
| topic | Alkaline lake Bacillus sp. Biodecolorization Strain CH12 Reactive Red 329 |
| url | http://www.sciencedirect.com/science/article/pii/S2215017X1630056X |
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