Storage duration of human blood samples for fatty acid concentration analyses - How long is too long?
Polyunsaturated fatty acids such as DHA have known anti-inflammatory properties. The therapeutic implication highlights the importance of accurate serum measurements. Sample preservation is challenging when performed parallel to the clinical obligations. Impact of time between sample collection and...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2024-06-01
|
| Series: | MethodsX |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2215016124000189 |
| _version_ | 1797351593507553280 |
|---|---|
| author | Ghaith Mohsen Helga Peisker Katharina Gutbrod Christian Stoppe Georg Daniel Duerr Markus Velten |
| author_facet | Ghaith Mohsen Helga Peisker Katharina Gutbrod Christian Stoppe Georg Daniel Duerr Markus Velten |
| author_sort | Ghaith Mohsen |
| collection | DOAJ |
| description | Polyunsaturated fatty acids such as DHA have known anti-inflammatory properties. The therapeutic implication highlights the importance of accurate serum measurements. Sample preservation is challenging when performed parallel to the clinical obligations. Impact of time between sample collection and processing regarding concentration alterations of fatty acids in human blood remains to be elucidated. Therefore, more information is required with respect to the stability and storage options in the context of potential degradation and concentration changes. This study investigates the stability of DHA in serum samples over time, given the challenges of timely sample analysis in clinical settings. Blood samples from three patients were collected and stored at +4 °C. Concentrations were analysed between 6 h and 7 days post-collection. Our data indicate that DHA concentrations remained unchanged during the observational period. Our results suggest that storage duration up to 7 days before sample processing does not affect accuracy of the results. DHA measurements is crucial for ongoing and future research in cardiovascular and inflammatory diseases. Our results reveal that DHA stability remains consistent over one week. This information is important for further clinical studies investigating PUFA concentrations, providing researches the option to postpone processing of samples if required along the clinical obligations. |
| first_indexed | 2024-03-08T13:02:43Z |
| format | Article |
| id | doaj.art-e3dbab9a8cda4a688df21198e9c3fa3a |
| institution | Directory Open Access Journal |
| issn | 2215-0161 |
| language | English |
| last_indexed | 2024-03-08T13:02:43Z |
| publishDate | 2024-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | MethodsX |
| spelling | doaj.art-e3dbab9a8cda4a688df21198e9c3fa3a2024-01-19T04:57:47ZengElsevierMethodsX2215-01612024-06-0112102564Storage duration of human blood samples for fatty acid concentration analyses - How long is too long?Ghaith Mohsen0Helga Peisker1Katharina Gutbrod2Christian Stoppe3Georg Daniel Duerr4Markus Velten5Department of Anesthesiology and Intensive Care Medicine, University Hospital Bonn, GermanyInstitute of Molecular Physiology and Biotechnology of Plants, University of Bonn, Bonn, GermanyInstitute of Molecular Physiology and Biotechnology of Plants, University of Bonn, Bonn, GermanyDepartment of Anesthesiology, Intensive Care, Emergency and Pain Medicine, University Hospital Würzburg, Würzburg, Germany; Department of Cardiac Anesthesiology and Intensive Care Medicine, Charité Berlin, Berlin, GermanyDepartment of Cardiovascular Surgery, University Medical Center Mainz, GermanyDepartment of Anesthesiology and Intensive Care Medicine, University Hospital Bonn, Germany; Department of Anesthesiology and Pain Management, University of Texas Southwestern Medical Center, Dallas, TX, USA; Corresponding author.Polyunsaturated fatty acids such as DHA have known anti-inflammatory properties. The therapeutic implication highlights the importance of accurate serum measurements. Sample preservation is challenging when performed parallel to the clinical obligations. Impact of time between sample collection and processing regarding concentration alterations of fatty acids in human blood remains to be elucidated. Therefore, more information is required with respect to the stability and storage options in the context of potential degradation and concentration changes. This study investigates the stability of DHA in serum samples over time, given the challenges of timely sample analysis in clinical settings. Blood samples from three patients were collected and stored at +4 °C. Concentrations were analysed between 6 h and 7 days post-collection. Our data indicate that DHA concentrations remained unchanged during the observational period. Our results suggest that storage duration up to 7 days before sample processing does not affect accuracy of the results. DHA measurements is crucial for ongoing and future research in cardiovascular and inflammatory diseases. Our results reveal that DHA stability remains consistent over one week. This information is important for further clinical studies investigating PUFA concentrations, providing researches the option to postpone processing of samples if required along the clinical obligations.http://www.sciencedirect.com/science/article/pii/S2215016124000189Storage duration human blood FA concentration |
| spellingShingle | Ghaith Mohsen Helga Peisker Katharina Gutbrod Christian Stoppe Georg Daniel Duerr Markus Velten Storage duration of human blood samples for fatty acid concentration analyses - How long is too long? MethodsX Storage duration human blood FA concentration |
| title | Storage duration of human blood samples for fatty acid concentration analyses - How long is too long? |
| title_full | Storage duration of human blood samples for fatty acid concentration analyses - How long is too long? |
| title_fullStr | Storage duration of human blood samples for fatty acid concentration analyses - How long is too long? |
| title_full_unstemmed | Storage duration of human blood samples for fatty acid concentration analyses - How long is too long? |
| title_short | Storage duration of human blood samples for fatty acid concentration analyses - How long is too long? |
| title_sort | storage duration of human blood samples for fatty acid concentration analyses how long is too long |
| topic | Storage duration human blood FA concentration |
| url | http://www.sciencedirect.com/science/article/pii/S2215016124000189 |
| work_keys_str_mv | AT ghaithmohsen storagedurationofhumanbloodsamplesforfattyacidconcentrationanalyseshowlongistoolong AT helgapeisker storagedurationofhumanbloodsamplesforfattyacidconcentrationanalyseshowlongistoolong AT katharinagutbrod storagedurationofhumanbloodsamplesforfattyacidconcentrationanalyseshowlongistoolong AT christianstoppe storagedurationofhumanbloodsamplesforfattyacidconcentrationanalyseshowlongistoolong AT georgdanielduerr storagedurationofhumanbloodsamplesforfattyacidconcentrationanalyseshowlongistoolong AT markusvelten storagedurationofhumanbloodsamplesforfattyacidconcentrationanalyseshowlongistoolong |