Susceptibility of Multidrug-Resistant and Biofilm-Forming Uropathogens to Mexican Oregano Essential Oil
Antibiotic resistance along with biofilm formation increases the difficulty for antibiotic therapy in urinary tract infections. Bioactive molecules derived from plants, such as those present in essential oils, can be used to treat bacterial infections. Oregano is one of the spices to have antimicrob...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2019-10-01
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| Series: | Antibiotics |
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| Online Access: | https://www.mdpi.com/2079-6382/8/4/186 |
| _version_ | 1811262938263060480 |
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| author | Karen Arely Zapién-Chavarría Alejandro Plascencia-Terrazas María Georgina Venegas-Ortega Mauricio Varillas-Torres Blanca Estela Rivera-Chavira Jaime Raúl Adame-Gallegos María Olga González-Rangel Guadalupe Virginia Nevárez-Moorillón |
| author_facet | Karen Arely Zapién-Chavarría Alejandro Plascencia-Terrazas María Georgina Venegas-Ortega Mauricio Varillas-Torres Blanca Estela Rivera-Chavira Jaime Raúl Adame-Gallegos María Olga González-Rangel Guadalupe Virginia Nevárez-Moorillón |
| author_sort | Karen Arely Zapién-Chavarría |
| collection | DOAJ |
| description | Antibiotic resistance along with biofilm formation increases the difficulty for antibiotic therapy in urinary tract infections. Bioactive molecules derived from plants, such as those present in essential oils, can be used to treat bacterial infections. Oregano is one of the spices to have antimicrobial activity. Therefore, three Mexican oregano essential oils (two <i>Lippia berlandieri</i> Schauer and one <i>Poliomintha longiflora</i>) were tested for antimicrobial capacity against multidrug-resistant, biofilm-forming bacterial isolates. Clinical isolates from urinary tract infections were tested for antibiotic resistance. Multidrug-resistant isolates were evaluated for biofilm formation, and Mexican oregano antimicrobial effect was determined by the minimal inhibitory (CMI) and minimal bactericidal concentrations (CMB). The selected isolates were identified by molecular phylogenetic analysis. Sixty-one isolates were included in the study; twenty were characterized as multidrug-resistant and from those, six were strong biofilm formers. Three isolates were identified as <i>Escherichia coli</i>, two as <i>Pseudomonas aeruginosa</i> and one as <i>Enterococcus faecalis</i> based on the phylogenetic analysis of 16 S rRNA gene sequences. The antimicrobial effect was bactericidal; <i>E. faecalis</i> was the most susceptible (<200 mg/L CMI/CMB), and <i>P. aeruginosa</i> was the most resistant (>2,000 mg/L CMI/CMB). There was a range of 500-1000 mg/L (CMI/CMB) for the <i>E. coli</i> isolates. Mexican oregano essential oils demonstrated antimicrobial efficacy against multidrug-resistant clinical isolates. |
| first_indexed | 2024-04-12T19:35:45Z |
| format | Article |
| id | doaj.art-e3dc5159febd435db92f2cc30be4f91f |
| institution | Directory Open Access Journal |
| issn | 2079-6382 |
| language | English |
| last_indexed | 2024-04-12T19:35:45Z |
| publishDate | 2019-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antibiotics |
| spelling | doaj.art-e3dc5159febd435db92f2cc30be4f91f2022-12-22T03:19:14ZengMDPI AGAntibiotics2079-63822019-10-018418610.3390/antibiotics8040186antibiotics8040186Susceptibility of Multidrug-Resistant and Biofilm-Forming Uropathogens to Mexican Oregano Essential OilKaren Arely Zapién-Chavarría0Alejandro Plascencia-Terrazas1María Georgina Venegas-Ortega2Mauricio Varillas-Torres3Blanca Estela Rivera-Chavira4Jaime Raúl Adame-Gallegos5María Olga González-Rangel6Guadalupe Virginia Nevárez-Moorillón7Facultad de Ciencias Químicas, Universidad Autónoma de Chihuahua, Circuito Universitario s/n Campus Universitario II, 31125 Chihuahua, Chihuahua, MexicoFacultad de Ciencias Químicas, Universidad Autónoma de Chihuahua, Circuito Universitario s/n Campus Universitario II, 31125 Chihuahua, Chihuahua, MexicoResearch Group of Bioprocesses and Bioproducts, Department of Food Research, School of Chemistry, Universidad Autónoma de Coahuila, 25280 Saltillo, Coahuila, MexicoFacultad de Ciencias Químicas, Universidad Autónoma de Chihuahua, Circuito Universitario s/n Campus Universitario II, 31125 Chihuahua, Chihuahua, MexicoFacultad de Ciencias Químicas, Universidad Autónoma de Chihuahua, Circuito Universitario s/n Campus Universitario II, 31125 Chihuahua, Chihuahua, MexicoFacultad de Ciencias Químicas, Universidad Autónoma de Chihuahua, Circuito Universitario s/n Campus Universitario II, 31125 Chihuahua, Chihuahua, MexicoFacultad de Ciencias Químicas, Universidad Autónoma de Chihuahua, Circuito Universitario s/n Campus Universitario II, 31125 Chihuahua, Chihuahua, MexicoFacultad de Ciencias Químicas, Universidad Autónoma de Chihuahua, Circuito Universitario s/n Campus Universitario II, 31125 Chihuahua, Chihuahua, MexicoAntibiotic resistance along with biofilm formation increases the difficulty for antibiotic therapy in urinary tract infections. Bioactive molecules derived from plants, such as those present in essential oils, can be used to treat bacterial infections. Oregano is one of the spices to have antimicrobial activity. Therefore, three Mexican oregano essential oils (two <i>Lippia berlandieri</i> Schauer and one <i>Poliomintha longiflora</i>) were tested for antimicrobial capacity against multidrug-resistant, biofilm-forming bacterial isolates. Clinical isolates from urinary tract infections were tested for antibiotic resistance. Multidrug-resistant isolates were evaluated for biofilm formation, and Mexican oregano antimicrobial effect was determined by the minimal inhibitory (CMI) and minimal bactericidal concentrations (CMB). The selected isolates were identified by molecular phylogenetic analysis. Sixty-one isolates were included in the study; twenty were characterized as multidrug-resistant and from those, six were strong biofilm formers. Three isolates were identified as <i>Escherichia coli</i>, two as <i>Pseudomonas aeruginosa</i> and one as <i>Enterococcus faecalis</i> based on the phylogenetic analysis of 16 S rRNA gene sequences. The antimicrobial effect was bactericidal; <i>E. faecalis</i> was the most susceptible (<200 mg/L CMI/CMB), and <i>P. aeruginosa</i> was the most resistant (>2,000 mg/L CMI/CMB). There was a range of 500-1000 mg/L (CMI/CMB) for the <i>E. coli</i> isolates. Mexican oregano essential oils demonstrated antimicrobial efficacy against multidrug-resistant clinical isolates.https://www.mdpi.com/2079-6382/8/4/186multidrug resistancemexican oreganobiofilm formationphylogenetic analysisurinary tract infections |
| spellingShingle | Karen Arely Zapién-Chavarría Alejandro Plascencia-Terrazas María Georgina Venegas-Ortega Mauricio Varillas-Torres Blanca Estela Rivera-Chavira Jaime Raúl Adame-Gallegos María Olga González-Rangel Guadalupe Virginia Nevárez-Moorillón Susceptibility of Multidrug-Resistant and Biofilm-Forming Uropathogens to Mexican Oregano Essential Oil Antibiotics multidrug resistance mexican oregano biofilm formation phylogenetic analysis urinary tract infections |
| title | Susceptibility of Multidrug-Resistant and Biofilm-Forming Uropathogens to Mexican Oregano Essential Oil |
| title_full | Susceptibility of Multidrug-Resistant and Biofilm-Forming Uropathogens to Mexican Oregano Essential Oil |
| title_fullStr | Susceptibility of Multidrug-Resistant and Biofilm-Forming Uropathogens to Mexican Oregano Essential Oil |
| title_full_unstemmed | Susceptibility of Multidrug-Resistant and Biofilm-Forming Uropathogens to Mexican Oregano Essential Oil |
| title_short | Susceptibility of Multidrug-Resistant and Biofilm-Forming Uropathogens to Mexican Oregano Essential Oil |
| title_sort | susceptibility of multidrug resistant and biofilm forming uropathogens to mexican oregano essential oil |
| topic | multidrug resistance mexican oregano biofilm formation phylogenetic analysis urinary tract infections |
| url | https://www.mdpi.com/2079-6382/8/4/186 |
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