Synergistic in vitro anticancer actions of decorated selenium nanoparticles with fucoidan/Reishi extract against colorectal adenocarcinoma cells
Cancer-fighting needs more effective and targeted drugs, desirably with least side-effects and from natural bases. The assessment and synergistic actions of multiple anticancer drug candidates were investigated. Fucoidan (Fu) was extracted from Sargassum cinereum, which was attained from the Saudi R...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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De Gruyter
2022-04-01
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| Series: | Green Processing and Synthesis |
| Subjects: | |
| Online Access: | https://doi.org/10.1515/gps-2022-0035 |
| _version_ | 1797998404136075264 |
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| author | El-Hefnawy Mohamed E. El-Sherbiny Mohsen M. Al Harbi Mamdouh Tayel Ahmed A. |
| author_facet | El-Hefnawy Mohamed E. El-Sherbiny Mohsen M. Al Harbi Mamdouh Tayel Ahmed A. |
| author_sort | El-Hefnawy Mohamed E. |
| collection | DOAJ |
| description | Cancer-fighting needs more effective and targeted drugs, desirably with least side-effects and from natural bases. The assessment and synergistic actions of multiple anticancer drug candidates were investigated. Fucoidan (Fu) was extracted from Sargassum cinereum, which was attained from the Saudi Red-Sea coast. The extract of Ganoderma lucidum or Reishi mushroom (Re) was achieved, intermixed with Fu solution, and they were used for reducing/decorating/capping selenium nanoparticles (SeNPs). The infrared analysis of produced/synthesized agents emphasized their biochemical structures and interactions. The decorated Fu/Re/SeNPs had negative (−30.6 mV) zeta potential and the SeNPs mean particle size was 6.5 nm. The transmission imaging of SeNPs indicated their spherical profiles and well-dispersion within Fu/Re composite. The anticancer potentiality of Fu/Re and Fu/Re/SeNPs against human colorectal adenocarcinoma cells was investigated using MTT, 4′,6-diamidino-2-phenylindole staining and comet assay. Both composites exhibited potent bioactivities toward adenocarcinoma cells; the reduced cells survival was detected with IC50 of 57.82 and 16.43 µg·mL−1 for Fu/Re and Fu/Re/SeNPs, respectively. The constrained apoptosis was notably observed from treated cells with the composites; substantial molecular damages were also verified via the comet assay, especially in Fu/Re/SeNPs treated cells. The innovative innocuous composite from Fu/Re/SeNPs is highly recommended to deactivate/destruct colorectal adenocarcinoma cells. |
| first_indexed | 2024-04-11T10:48:15Z |
| format | Article |
| id | doaj.art-e3dd169ad7ac421a9c47c162e09582ce |
| institution | Directory Open Access Journal |
| issn | 2191-9550 |
| language | English |
| last_indexed | 2024-04-11T10:48:15Z |
| publishDate | 2022-04-01 |
| publisher | De Gruyter |
| record_format | Article |
| series | Green Processing and Synthesis |
| spelling | doaj.art-e3dd169ad7ac421a9c47c162e09582ce2022-12-22T04:29:00ZengDe GruyterGreen Processing and Synthesis2191-95502022-04-0111137338410.1515/gps-2022-0035Synergistic in vitro anticancer actions of decorated selenium nanoparticles with fucoidan/Reishi extract against colorectal adenocarcinoma cellsEl-Hefnawy Mohamed E.0El-Sherbiny Mohsen M.1Al Harbi Mamdouh2Tayel Ahmed A.3Department of Chemistry, Rabigh College of Sciences and Arts, King Abdulaziz University, Jeddah 21589, Saudi ArabiaMarine Biology Department, Faculty of Marine Sciences, King Abdulaziz University, Jeddah 21589, Saudi ArabiaMarine Biology Department, Faculty of Marine Sciences, King Abdulaziz University, Jeddah 21589, Saudi ArabiaDepartment of Fish Processing and Biotechnology, Faculty of Aquatic and Fisheries Sciences, Kafrelsheikh University, Kafrelsheikh 33516, EgyptCancer-fighting needs more effective and targeted drugs, desirably with least side-effects and from natural bases. The assessment and synergistic actions of multiple anticancer drug candidates were investigated. Fucoidan (Fu) was extracted from Sargassum cinereum, which was attained from the Saudi Red-Sea coast. The extract of Ganoderma lucidum or Reishi mushroom (Re) was achieved, intermixed with Fu solution, and they were used for reducing/decorating/capping selenium nanoparticles (SeNPs). The infrared analysis of produced/synthesized agents emphasized their biochemical structures and interactions. The decorated Fu/Re/SeNPs had negative (−30.6 mV) zeta potential and the SeNPs mean particle size was 6.5 nm. The transmission imaging of SeNPs indicated their spherical profiles and well-dispersion within Fu/Re composite. The anticancer potentiality of Fu/Re and Fu/Re/SeNPs against human colorectal adenocarcinoma cells was investigated using MTT, 4′,6-diamidino-2-phenylindole staining and comet assay. Both composites exhibited potent bioactivities toward adenocarcinoma cells; the reduced cells survival was detected with IC50 of 57.82 and 16.43 µg·mL−1 for Fu/Re and Fu/Re/SeNPs, respectively. The constrained apoptosis was notably observed from treated cells with the composites; substantial molecular damages were also verified via the comet assay, especially in Fu/Re/SeNPs treated cells. The innovative innocuous composite from Fu/Re/SeNPs is highly recommended to deactivate/destruct colorectal adenocarcinoma cells.https://doi.org/10.1515/gps-2022-0035antitumor activitybioactive polymersbiosynthesisht-29 cellsmode of action |
| spellingShingle | El-Hefnawy Mohamed E. El-Sherbiny Mohsen M. Al Harbi Mamdouh Tayel Ahmed A. Synergistic in vitro anticancer actions of decorated selenium nanoparticles with fucoidan/Reishi extract against colorectal adenocarcinoma cells Green Processing and Synthesis antitumor activity bioactive polymers biosynthesis ht-29 cells mode of action |
| title | Synergistic in vitro anticancer actions of decorated selenium nanoparticles with fucoidan/Reishi extract against colorectal adenocarcinoma cells |
| title_full | Synergistic in vitro anticancer actions of decorated selenium nanoparticles with fucoidan/Reishi extract against colorectal adenocarcinoma cells |
| title_fullStr | Synergistic in vitro anticancer actions of decorated selenium nanoparticles with fucoidan/Reishi extract against colorectal adenocarcinoma cells |
| title_full_unstemmed | Synergistic in vitro anticancer actions of decorated selenium nanoparticles with fucoidan/Reishi extract against colorectal adenocarcinoma cells |
| title_short | Synergistic in vitro anticancer actions of decorated selenium nanoparticles with fucoidan/Reishi extract against colorectal adenocarcinoma cells |
| title_sort | synergistic in vitro anticancer actions of decorated selenium nanoparticles with fucoidan reishi extract against colorectal adenocarcinoma cells |
| topic | antitumor activity bioactive polymers biosynthesis ht-29 cells mode of action |
| url | https://doi.org/10.1515/gps-2022-0035 |
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