Antihypertensive effect of the stem bark aqueous extract of Garcinia lucida Vesque (Clusiaceae) in L-NAME-treated rats: Contribution of endothelium-dependent and -independent vasorelaxation
Garcinia lucida is used in Cameroonian folk medicine to handle a variety of ailments, including arterial hypertension. This study aimed at determining the phytochemical profile and the antihypertensive effect of the stem bark aqueous extract of G. lucida (AEGL). AEGL was subjected to LC-MS analysis,...
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Elsevier
2023-11-01
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405844023091041 |
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| author | Elvine Pami Nguelefack-Mbuyo Christelle Stéphanie Sonfack Christian Kuété Fofié Chamberlin Fodem Magloire Kanyou Ndjenda II Alain Bertrand Dongmo Télesphore Benoît Nguelefack |
| author_facet | Elvine Pami Nguelefack-Mbuyo Christelle Stéphanie Sonfack Christian Kuété Fofié Chamberlin Fodem Magloire Kanyou Ndjenda II Alain Bertrand Dongmo Télesphore Benoît Nguelefack |
| author_sort | Elvine Pami Nguelefack-Mbuyo |
| collection | DOAJ |
| description | Garcinia lucida is used in Cameroonian folk medicine to handle a variety of ailments, including arterial hypertension. This study aimed at determining the phytochemical profile and the antihypertensive effect of the stem bark aqueous extract of G. lucida (AEGL). AEGL was subjected to LC-MS analysis, and its effect (75, 150, and 300 mg/kg/day; by gavage) was evaluated against Nω-nitro-L-arginine methyl ester (L-NAME; 40 mg/kg)-induced hypertension in adult male Wistar rats for four consecutive weeks. Blood pressure and heart rate were monitored weekly using tail-cuff plethysmography. The vasorelaxant effect of cumulative concentrations (3–10–30–100–300 μg/mL) of AEGL was examined on endothelium-intact and denuded thoracic aorta rings which were precontracted with KCl (90 mM) or norepinephrine (NE; 10−5 M), and in the absence or presence of L-NAME (10−4 M), indomethacin (10−5 M), methylene blue (10−6 M), tetraethylammonium (TEA, 5 × 10−6 M), glibenclamide (10 × 10−6 M) or propranolol (5 × 10−6 M). The influence of AEGL on the response to NE, KCl, and CaCl2 was also investigated. Six compounds, including Garcinia biflavonoids GB1 and GB2, were identified. AEGL prevented the development of hypertension (p < 0.01 and p < 0.001) without affecting the heart rate. AEGL induced a concentration-dependent relaxation of aortic rings precontracted with NE (EC50 = 7.915 μg/mL) that was significantly inhibited by the removal of the endothelium, L-NAME, or methylene blue (p < 0.05–0.001). Indomethacin, propranolol, TEA, and glibenclamide did not affect AEGL-evoked vasorelaxation. Preincubation of aortic rings with AEGL reduced the magnitude of contraction elicited by CaCl2 but did not alter that of KCl or NE. AEGL possesses an antihypertensive effect that is mediated by both endothelium-dependent and endothelium-independent mechanisms. The activation of the NO/sGC/cGMP pathway accounts for the endothelium-dependent vasorelaxation. These pharmacological effects of AEGL could be attributed to the presence of the Garcinia biflavonoids GB1 and GB2. |
| first_indexed | 2024-03-09T09:18:16Z |
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| institution | Directory Open Access Journal |
| issn | 2405-8440 |
| language | English |
| last_indexed | 2024-03-09T09:18:16Z |
| publishDate | 2023-11-01 |
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| spelling | doaj.art-e3e040e3dd0449fb8f1b0e58a3c33eb42023-12-02T07:04:36ZengElsevierHeliyon2405-84402023-11-01911e21896Antihypertensive effect of the stem bark aqueous extract of Garcinia lucida Vesque (Clusiaceae) in L-NAME-treated rats: Contribution of endothelium-dependent and -independent vasorelaxationElvine Pami Nguelefack-Mbuyo0Christelle Stéphanie Sonfack1Christian Kuété Fofié2Chamberlin Fodem3Magloire Kanyou Ndjenda II4Alain Bertrand Dongmo5Télesphore Benoît Nguelefack6Laboratory of Animal Physiology and Phytopharmacology, Faculty of Science, University of Dschang, P.O. Box 67, Dschang, Cameroon; Corresponding author.Laboratory of Animal Physiology and Phytopharmacology, Faculty of Science, University of Dschang, P.O. Box 67, Dschang, Cameroon; Department of Animal Biology and Physiology, Faculty of Sciences, University of Douala, P.O. Box 24157, CameroonLaboratory of Animal Physiology and Phytopharmacology, Faculty of Science, University of Dschang, P.O. Box 67, Dschang, CameroonLaboratory of Animal Physiology and Phytopharmacology, Faculty of Science, University of Dschang, P.O. Box 67, Dschang, CameroonLaboratory of Animal Physiology and Phytopharmacology, Faculty of Science, University of Dschang, P.O. Box 67, Dschang, CameroonDepartment of Animal Biology and Physiology, Faculty of Sciences, University of Douala, P.O. Box 24157, CameroonLaboratory of Animal Physiology and Phytopharmacology, Faculty of Science, University of Dschang, P.O. Box 67, Dschang, CameroonGarcinia lucida is used in Cameroonian folk medicine to handle a variety of ailments, including arterial hypertension. This study aimed at determining the phytochemical profile and the antihypertensive effect of the stem bark aqueous extract of G. lucida (AEGL). AEGL was subjected to LC-MS analysis, and its effect (75, 150, and 300 mg/kg/day; by gavage) was evaluated against Nω-nitro-L-arginine methyl ester (L-NAME; 40 mg/kg)-induced hypertension in adult male Wistar rats for four consecutive weeks. Blood pressure and heart rate were monitored weekly using tail-cuff plethysmography. The vasorelaxant effect of cumulative concentrations (3–10–30–100–300 μg/mL) of AEGL was examined on endothelium-intact and denuded thoracic aorta rings which were precontracted with KCl (90 mM) or norepinephrine (NE; 10−5 M), and in the absence or presence of L-NAME (10−4 M), indomethacin (10−5 M), methylene blue (10−6 M), tetraethylammonium (TEA, 5 × 10−6 M), glibenclamide (10 × 10−6 M) or propranolol (5 × 10−6 M). The influence of AEGL on the response to NE, KCl, and CaCl2 was also investigated. Six compounds, including Garcinia biflavonoids GB1 and GB2, were identified. AEGL prevented the development of hypertension (p < 0.01 and p < 0.001) without affecting the heart rate. AEGL induced a concentration-dependent relaxation of aortic rings precontracted with NE (EC50 = 7.915 μg/mL) that was significantly inhibited by the removal of the endothelium, L-NAME, or methylene blue (p < 0.05–0.001). Indomethacin, propranolol, TEA, and glibenclamide did not affect AEGL-evoked vasorelaxation. Preincubation of aortic rings with AEGL reduced the magnitude of contraction elicited by CaCl2 but did not alter that of KCl or NE. AEGL possesses an antihypertensive effect that is mediated by both endothelium-dependent and endothelium-independent mechanisms. The activation of the NO/sGC/cGMP pathway accounts for the endothelium-dependent vasorelaxation. These pharmacological effects of AEGL could be attributed to the presence of the Garcinia biflavonoids GB1 and GB2.http://www.sciencedirect.com/science/article/pii/S2405844023091041Antihypertensive effectGarcinia biflavonoidsGarcinia lucidaLC-MS analysisVasorelaxant effect |
| spellingShingle | Elvine Pami Nguelefack-Mbuyo Christelle Stéphanie Sonfack Christian Kuété Fofié Chamberlin Fodem Magloire Kanyou Ndjenda II Alain Bertrand Dongmo Télesphore Benoît Nguelefack Antihypertensive effect of the stem bark aqueous extract of Garcinia lucida Vesque (Clusiaceae) in L-NAME-treated rats: Contribution of endothelium-dependent and -independent vasorelaxation Heliyon Antihypertensive effect Garcinia biflavonoids Garcinia lucida LC-MS analysis Vasorelaxant effect |
| title | Antihypertensive effect of the stem bark aqueous extract of Garcinia lucida Vesque (Clusiaceae) in L-NAME-treated rats: Contribution of endothelium-dependent and -independent vasorelaxation |
| title_full | Antihypertensive effect of the stem bark aqueous extract of Garcinia lucida Vesque (Clusiaceae) in L-NAME-treated rats: Contribution of endothelium-dependent and -independent vasorelaxation |
| title_fullStr | Antihypertensive effect of the stem bark aqueous extract of Garcinia lucida Vesque (Clusiaceae) in L-NAME-treated rats: Contribution of endothelium-dependent and -independent vasorelaxation |
| title_full_unstemmed | Antihypertensive effect of the stem bark aqueous extract of Garcinia lucida Vesque (Clusiaceae) in L-NAME-treated rats: Contribution of endothelium-dependent and -independent vasorelaxation |
| title_short | Antihypertensive effect of the stem bark aqueous extract of Garcinia lucida Vesque (Clusiaceae) in L-NAME-treated rats: Contribution of endothelium-dependent and -independent vasorelaxation |
| title_sort | antihypertensive effect of the stem bark aqueous extract of garcinia lucida vesque clusiaceae in l name treated rats contribution of endothelium dependent and independent vasorelaxation |
| topic | Antihypertensive effect Garcinia biflavonoids Garcinia lucida LC-MS analysis Vasorelaxant effect |
| url | http://www.sciencedirect.com/science/article/pii/S2405844023091041 |
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