Diversity and Antimicrobial Potential of Predatory Bacteria from the Peruvian Coastline
The microbiome of three different sites at the Peruvian Pacific coast was analyzed, revealing a lower bacterial biodiversity at Isla Foca than at Paracas and Manglares, with 89 bacterial genera identified, as compared to 195 and 173 genera, respectively. Only 47 of the bacterial genera identified we...
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| Format: | Article |
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MDPI AG
2017-10-01
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| Series: | Marine Drugs |
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| Online Access: | https://www.mdpi.com/1660-3397/15/10/308 |
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| author | Luis Linares-Otoya Virginia Linares-Otoya Lizbeth Armas-Mantilla Cyntia Blanco-Olano Max Crüsemann Mayar L. Ganoza-Yupanqui Julio Campos-Florian Gabriele M. König Till F. Schäberle |
| author_facet | Luis Linares-Otoya Virginia Linares-Otoya Lizbeth Armas-Mantilla Cyntia Blanco-Olano Max Crüsemann Mayar L. Ganoza-Yupanqui Julio Campos-Florian Gabriele M. König Till F. Schäberle |
| author_sort | Luis Linares-Otoya |
| collection | DOAJ |
| description | The microbiome of three different sites at the Peruvian Pacific coast was analyzed, revealing a lower bacterial biodiversity at Isla Foca than at Paracas and Manglares, with 89 bacterial genera identified, as compared to 195 and 173 genera, respectively. Only 47 of the bacterial genera identified were common to all three sites. In order to obtain promising strains for the putative production of novel antimicrobials, predatory bacteria were isolated from these sampling sites, using two different bait organisms. Even though the proportion of predatory bacteria was only around 0.5% in the here investigated environmental microbiomes, by this approach in total 138 bacterial strains were isolated as axenic culture. 25% of strains showed antibacterial activity, thereby nine revealed activity against clinically relevant methicillin resistant Staphylococcus aureus (MRSA) and three against enterohemorrhagic Escherichia coli (EHEC) strains. Phylogeny and physiological characteristics of the active strains were investigated. First insights into the chemical basis of the antibacterial activity indicated the biosynthetic production of the known compounds ariakemicin, kocurin, naphthyridinomycin, pumilacidins, resistomycin, and surfactin. However, most compounds remained elusive until now. Hence, the obtained results implicate that the microbiome present at the various habitats at the Peruvian coastline is a promising source for heterotrophic bacterial strains showing high potential for the biotechnological production of antibiotics. |
| first_indexed | 2024-04-11T13:12:00Z |
| format | Article |
| id | doaj.art-e3e1297efc0146729bf2d805434e18e2 |
| institution | Directory Open Access Journal |
| issn | 1660-3397 |
| language | English |
| last_indexed | 2024-04-11T13:12:00Z |
| publishDate | 2017-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Marine Drugs |
| spelling | doaj.art-e3e1297efc0146729bf2d805434e18e22022-12-22T04:22:34ZengMDPI AGMarine Drugs1660-33972017-10-01151030810.3390/md15100308md15100308Diversity and Antimicrobial Potential of Predatory Bacteria from the Peruvian CoastlineLuis Linares-Otoya0Virginia Linares-Otoya1Lizbeth Armas-Mantilla2Cyntia Blanco-Olano3Max Crüsemann4Mayar L. Ganoza-Yupanqui5Julio Campos-Florian6Gabriele M. König7Till F. Schäberle8Institute for Insect Biotechnology, Justus Liebig University of Giessen, 5392 Giessen, GermanyDepartment of Pharmacology, Faculty of Pharmacy and Biochemistry, National University of Trujillo, 13011 Trujillo, PeruDepartment of Pharmacology, Faculty of Pharmacy and Biochemistry, National University of Trujillo, 13011 Trujillo, PeruDepartment of Pharmacology, Faculty of Pharmacy and Biochemistry, National University of Trujillo, 13011 Trujillo, PeruInstitute for Pharmaceutical Biology, University of Bonn, 3115 Bonn, GermanyDepartment of Pharmacology, Faculty of Pharmacy and Biochemistry, National University of Trujillo, 13011 Trujillo, PeruDepartment of Pharmacology, Faculty of Pharmacy and Biochemistry, National University of Trujillo, 13011 Trujillo, PeruInstitute for Pharmaceutical Biology, University of Bonn, 3115 Bonn, GermanyInstitute for Insect Biotechnology, Justus Liebig University of Giessen, 5392 Giessen, GermanyThe microbiome of three different sites at the Peruvian Pacific coast was analyzed, revealing a lower bacterial biodiversity at Isla Foca than at Paracas and Manglares, with 89 bacterial genera identified, as compared to 195 and 173 genera, respectively. Only 47 of the bacterial genera identified were common to all three sites. In order to obtain promising strains for the putative production of novel antimicrobials, predatory bacteria were isolated from these sampling sites, using two different bait organisms. Even though the proportion of predatory bacteria was only around 0.5% in the here investigated environmental microbiomes, by this approach in total 138 bacterial strains were isolated as axenic culture. 25% of strains showed antibacterial activity, thereby nine revealed activity against clinically relevant methicillin resistant Staphylococcus aureus (MRSA) and three against enterohemorrhagic Escherichia coli (EHEC) strains. Phylogeny and physiological characteristics of the active strains were investigated. First insights into the chemical basis of the antibacterial activity indicated the biosynthetic production of the known compounds ariakemicin, kocurin, naphthyridinomycin, pumilacidins, resistomycin, and surfactin. However, most compounds remained elusive until now. Hence, the obtained results implicate that the microbiome present at the various habitats at the Peruvian coastline is a promising source for heterotrophic bacterial strains showing high potential for the biotechnological production of antibiotics.https://www.mdpi.com/1660-3397/15/10/308antibioticsdereplicationmicrobiomenatural productspredatory bacteria |
| spellingShingle | Luis Linares-Otoya Virginia Linares-Otoya Lizbeth Armas-Mantilla Cyntia Blanco-Olano Max Crüsemann Mayar L. Ganoza-Yupanqui Julio Campos-Florian Gabriele M. König Till F. Schäberle Diversity and Antimicrobial Potential of Predatory Bacteria from the Peruvian Coastline Marine Drugs antibiotics dereplication microbiome natural products predatory bacteria |
| title | Diversity and Antimicrobial Potential of Predatory Bacteria from the Peruvian Coastline |
| title_full | Diversity and Antimicrobial Potential of Predatory Bacteria from the Peruvian Coastline |
| title_fullStr | Diversity and Antimicrobial Potential of Predatory Bacteria from the Peruvian Coastline |
| title_full_unstemmed | Diversity and Antimicrobial Potential of Predatory Bacteria from the Peruvian Coastline |
| title_short | Diversity and Antimicrobial Potential of Predatory Bacteria from the Peruvian Coastline |
| title_sort | diversity and antimicrobial potential of predatory bacteria from the peruvian coastline |
| topic | antibiotics dereplication microbiome natural products predatory bacteria |
| url | https://www.mdpi.com/1660-3397/15/10/308 |
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