Transcriptomic Profiling of Femoral Veins in Deep Vein Thrombosis in a Porcine Model
Deep vein thrombosis (DVT) is a severe disease affecting the human venous system, accompanied by high morbidity and mortality rates caused by early and late complications. The study aimed at analyzing the changes in the transcriptome of the femoral vein caused by DVT in the porcine model based on th...
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MDPI AG
2021-06-01
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| author | Leszek Gromadziński Łukasz Paukszto Agnieszka Skowrońska Piotr Holak Michał Smoliński Elżbieta Łopieńska-Biernat Ewa Lepiarczyk Aleksandra Lipka Jan Paweł Jastrzębski Marta Majewska |
| author_facet | Leszek Gromadziński Łukasz Paukszto Agnieszka Skowrońska Piotr Holak Michał Smoliński Elżbieta Łopieńska-Biernat Ewa Lepiarczyk Aleksandra Lipka Jan Paweł Jastrzębski Marta Majewska |
| author_sort | Leszek Gromadziński |
| collection | DOAJ |
| description | Deep vein thrombosis (DVT) is a severe disease affecting the human venous system, accompanied by high morbidity and mortality rates caused by early and late complications. The study aimed at analyzing the changes in the transcriptome of the femoral vein caused by DVT in the porcine model based on the formation of the thrombus in vivo. The study was performed on 11 castrated male pigs: A thrombus was formed in each left femoral vein in six animals; the remaining five served as a control group. Total RNA was isolated from the left femoral veins of the experimental and control animals. High-throughput RNA sequencing was used to analyze the global changes in the transcriptome of veins with induced DVT. Applied multistep bioinformatics revealed 1474 differentially expressed genes (DEGs): 1019 upregulated and 455 downregulated. Functional Gene Ontology annotated 1220 of DEGs into 225 biological processes, 30 molecular functions and 40 cellular components categories. KEGG analysis disclosed TNF, NF-κB and apoptosis pathways’ overexpression in DVT samples. A thorough analysis of the detected DEGs indicated that a dysregulated inflammatory response and disturbed balance between clotting and anti-clotting factors play a crucial role in the process of DVT. |
| first_indexed | 2024-03-10T10:11:10Z |
| format | Article |
| id | doaj.art-e3e13fc7780e48f18793dbaea500bf5d |
| institution | Directory Open Access Journal |
| issn | 2073-4409 |
| language | English |
| last_indexed | 2024-03-10T10:11:10Z |
| publishDate | 2021-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Cells |
| spelling | doaj.art-e3e13fc7780e48f18793dbaea500bf5d2023-11-22T01:14:42ZengMDPI AGCells2073-44092021-06-01107157610.3390/cells10071576Transcriptomic Profiling of Femoral Veins in Deep Vein Thrombosis in a Porcine ModelLeszek Gromadziński0Łukasz Paukszto1Agnieszka Skowrońska2Piotr Holak3Michał Smoliński4Elżbieta Łopieńska-Biernat5Ewa Lepiarczyk6Aleksandra Lipka7Jan Paweł Jastrzębski8Marta Majewska9Department of Cardiology and Internal Medicine, School of Medicine, Collegium Medicum, University of Warmia and Mazury in Olsztyn, 10-082 Olsztyn, PolandDepartment of Plant Physiology, Genetics and Biotechnology, Faculty of Biology and Biotechnology, University of Warmia and Mazury in Olsztyn, 10-719 Olsztyn, PolandDepartment of Human Physiology and Pathophysiology, School of Medicine, Collegium Medicum, University of Warmia and Mazury in Olsztyn, 10-082 Olsztyn, PolandDepartment of Surgery and Radiology with Clinic, Faculty of Veterinary Medicine, University of Warmia and Mazury in Olsztyn, 10-719 Olsztyn, PolandClinic of Cardiology and Internal Diseases, University Clinical Hospital in Olsztyn, 10-082 Olsztyn, PolandDepartment of Biochemistry, Faculty of Biology and Biotechnology, University of Warmia and Mazury in Olsztyn, 10-719 Olsztyn, PolandDepartment of Human Physiology and Pathophysiology, School of Medicine, Collegium Medicum, University of Warmia and Mazury in Olsztyn, 10-082 Olsztyn, PolandDepartment of Gynecology and Obstetrics, School of Medicine, Collegium Medicum, University of Warmia and Mazury in Olsztyn, 10-561 Olsztyn, PolandDepartment of Plant Physiology, Genetics and Biotechnology, Faculty of Biology and Biotechnology, University of Warmia and Mazury in Olsztyn, 10-719 Olsztyn, PolandDepartment of Human Physiology and Pathophysiology, School of Medicine, Collegium Medicum, University of Warmia and Mazury in Olsztyn, 10-082 Olsztyn, PolandDeep vein thrombosis (DVT) is a severe disease affecting the human venous system, accompanied by high morbidity and mortality rates caused by early and late complications. The study aimed at analyzing the changes in the transcriptome of the femoral vein caused by DVT in the porcine model based on the formation of the thrombus in vivo. The study was performed on 11 castrated male pigs: A thrombus was formed in each left femoral vein in six animals; the remaining five served as a control group. Total RNA was isolated from the left femoral veins of the experimental and control animals. High-throughput RNA sequencing was used to analyze the global changes in the transcriptome of veins with induced DVT. Applied multistep bioinformatics revealed 1474 differentially expressed genes (DEGs): 1019 upregulated and 455 downregulated. Functional Gene Ontology annotated 1220 of DEGs into 225 biological processes, 30 molecular functions and 40 cellular components categories. KEGG analysis disclosed TNF, NF-κB and apoptosis pathways’ overexpression in DVT samples. A thorough analysis of the detected DEGs indicated that a dysregulated inflammatory response and disturbed balance between clotting and anti-clotting factors play a crucial role in the process of DVT.https://www.mdpi.com/2073-4409/10/7/1576deep vein thrombosisRNA-seqgene expression and regulation |
| spellingShingle | Leszek Gromadziński Łukasz Paukszto Agnieszka Skowrońska Piotr Holak Michał Smoliński Elżbieta Łopieńska-Biernat Ewa Lepiarczyk Aleksandra Lipka Jan Paweł Jastrzębski Marta Majewska Transcriptomic Profiling of Femoral Veins in Deep Vein Thrombosis in a Porcine Model Cells deep vein thrombosis RNA-seq gene expression and regulation |
| title | Transcriptomic Profiling of Femoral Veins in Deep Vein Thrombosis in a Porcine Model |
| title_full | Transcriptomic Profiling of Femoral Veins in Deep Vein Thrombosis in a Porcine Model |
| title_fullStr | Transcriptomic Profiling of Femoral Veins in Deep Vein Thrombosis in a Porcine Model |
| title_full_unstemmed | Transcriptomic Profiling of Femoral Veins in Deep Vein Thrombosis in a Porcine Model |
| title_short | Transcriptomic Profiling of Femoral Veins in Deep Vein Thrombosis in a Porcine Model |
| title_sort | transcriptomic profiling of femoral veins in deep vein thrombosis in a porcine model |
| topic | deep vein thrombosis RNA-seq gene expression and regulation |
| url | https://www.mdpi.com/2073-4409/10/7/1576 |
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