Circulating microRNAs as Potential Novel Diagnostic Biomarkers to Predict Drug Resistance in Temporal Lobe Epilepsy: A Pilot Study
MicroRNAs (miRNAs) are small noncoding RNAs that have emerged as new potential epigenetic biomarkers. Here, we evaluate the efficacy of six circulating miRNA previously described in the literature as biomarkers for the diagnosis of temporal lobe epilepsy (TLE) and/or as predictive biomarkers to anti...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-01-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/22/2/702 |
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| author | Selene De Benedittis Francesco Fortunato Claudia Cava Francesca Gallivanone Enrico Iaccino Maria Eugenia Caligiuri Isabella Castiglioni Gloria Bertoli Ida Manna Angelo Labate Antonio Gambardella |
| author_facet | Selene De Benedittis Francesco Fortunato Claudia Cava Francesca Gallivanone Enrico Iaccino Maria Eugenia Caligiuri Isabella Castiglioni Gloria Bertoli Ida Manna Angelo Labate Antonio Gambardella |
| author_sort | Selene De Benedittis |
| collection | DOAJ |
| description | MicroRNAs (miRNAs) are small noncoding RNAs that have emerged as new potential epigenetic biomarkers. Here, we evaluate the efficacy of six circulating miRNA previously described in the literature as biomarkers for the diagnosis of temporal lobe epilepsy (TLE) and/or as predictive biomarkers to antiepileptic drug response. We measured the differences in serum miRNA levels by quantitative reverse transcriptase polymerase chain reaction (qRT-PCR) assays in a cohort of 27 patients (14 women and 13 men; mean ± SD age: 43.65 ± 17.07) with TLE compared to 20 healthy controls (HC) matched for sex, age and ethnicity (11 women and 9 men; mean ± SD age: 47.5 ± 9.1). Additionally, patients were classified according to whether they had drug-responsive (<i>n</i> = 17) or drug-resistant (<i>n</i> = 10) TLE. We have investigated any correlations between miRNAs and several electroclinical parameters. Three miRNAs (miR-142, miR-146a, miR-223) were significantly upregulated in patients (expressed as average expression ± SD). In detail, miR-142 expression was 0.40 ± 0.29 vs. 0.16 ± 0.10 in TLE patients compared to HC (<i>t</i>-test, <i>p</i> < 0.01), miR-146a expression was 0.15 ± 0.11 vs. 0.07 ± 0.04 (<i>t</i>-test, <i>p</i> < 0.05), and miR-223 expression was 6.21 ± 3.65 vs. 1.23 ± 0.84 (<i>t</i>-test, <i>p</i> < 0.001). Moreover, results obtained from a logistic regression model showed the good performance of miR-142 and miR-223 in distinguishing drug-sensitive vs. drug-resistant TLE. The results of this pilot study give evidence that miRNAs are suitable targets in TLE and offer the rationale for further confirmation studies in larger epilepsy cohorts. |
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| format | Article |
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| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-09T05:02:33Z |
| publishDate | 2021-01-01 |
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| spelling | doaj.art-e3e1cd38aed348a6bd902ac43a6f717f2023-12-03T12:58:42ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672021-01-0122270210.3390/ijms22020702Circulating microRNAs as Potential Novel Diagnostic Biomarkers to Predict Drug Resistance in Temporal Lobe Epilepsy: A Pilot StudySelene De Benedittis0Francesco Fortunato1Claudia Cava2Francesca Gallivanone3Enrico Iaccino4Maria Eugenia Caligiuri5Isabella Castiglioni6Gloria Bertoli7Ida Manna8Angelo Labate9Antonio Gambardella10Department of Medical and Surgical Sciences, Institute of Neurology, University “Magna Graecia”, Germaneto, 88100 Catanzaro, ItalyDepartment of Medical and Surgical Sciences, Institute of Neurology, University “Magna Graecia”, Germaneto, 88100 Catanzaro, ItalyInstitute of Molecular Bioimaging and Physiology (IBFM), National Research Council (CNR), Via F.Cervi 93, 20090 Segrate-Milan, ItalyInstitute of Molecular Bioimaging and Physiology (IBFM), National Research Council (CNR), Via F.Cervi 93, 20090 Segrate-Milan, ItalyDepartment of Experimental and Clinical Medicine, University “Magna Graecia” of Catanzaro, 88100 Catanzaro, ItalyNeuroscience Research Center, University “Magna Graecia” of Catanzaro, 88100 Catanzaro, ItalyDepartment of Physics “Giuseppe Occhialini”, University of Milan-Bicocca, Piazza della Scienza 3, 20126 Milan, ItalyInstitute of Molecular Bioimaging and Physiology (IBFM), National Research Council (CNR), Via F.Cervi 93, 20090 Segrate-Milan, ItalyInstitute of Molecular Bioimaging and Physiology (IBFM), National Research Council (CNR), Section of Germaneto, 88100 Catanzaro, ItalyDepartment of Medical and Surgical Sciences, Institute of Neurology, University “Magna Graecia”, Germaneto, 88100 Catanzaro, ItalyDepartment of Medical and Surgical Sciences, Institute of Neurology, University “Magna Graecia”, Germaneto, 88100 Catanzaro, ItalyMicroRNAs (miRNAs) are small noncoding RNAs that have emerged as new potential epigenetic biomarkers. Here, we evaluate the efficacy of six circulating miRNA previously described in the literature as biomarkers for the diagnosis of temporal lobe epilepsy (TLE) and/or as predictive biomarkers to antiepileptic drug response. We measured the differences in serum miRNA levels by quantitative reverse transcriptase polymerase chain reaction (qRT-PCR) assays in a cohort of 27 patients (14 women and 13 men; mean ± SD age: 43.65 ± 17.07) with TLE compared to 20 healthy controls (HC) matched for sex, age and ethnicity (11 women and 9 men; mean ± SD age: 47.5 ± 9.1). Additionally, patients were classified according to whether they had drug-responsive (<i>n</i> = 17) or drug-resistant (<i>n</i> = 10) TLE. We have investigated any correlations between miRNAs and several electroclinical parameters. Three miRNAs (miR-142, miR-146a, miR-223) were significantly upregulated in patients (expressed as average expression ± SD). In detail, miR-142 expression was 0.40 ± 0.29 vs. 0.16 ± 0.10 in TLE patients compared to HC (<i>t</i>-test, <i>p</i> < 0.01), miR-146a expression was 0.15 ± 0.11 vs. 0.07 ± 0.04 (<i>t</i>-test, <i>p</i> < 0.05), and miR-223 expression was 6.21 ± 3.65 vs. 1.23 ± 0.84 (<i>t</i>-test, <i>p</i> < 0.001). Moreover, results obtained from a logistic regression model showed the good performance of miR-142 and miR-223 in distinguishing drug-sensitive vs. drug-resistant TLE. The results of this pilot study give evidence that miRNAs are suitable targets in TLE and offer the rationale for further confirmation studies in larger epilepsy cohorts.https://www.mdpi.com/1422-0067/22/2/702temporal lobe epilepsymiRNAsdiagnosisprognosisantiseizure medicationsASMs |
| spellingShingle | Selene De Benedittis Francesco Fortunato Claudia Cava Francesca Gallivanone Enrico Iaccino Maria Eugenia Caligiuri Isabella Castiglioni Gloria Bertoli Ida Manna Angelo Labate Antonio Gambardella Circulating microRNAs as Potential Novel Diagnostic Biomarkers to Predict Drug Resistance in Temporal Lobe Epilepsy: A Pilot Study International Journal of Molecular Sciences temporal lobe epilepsy miRNAs diagnosis prognosis antiseizure medications ASMs |
| title | Circulating microRNAs as Potential Novel Diagnostic Biomarkers to Predict Drug Resistance in Temporal Lobe Epilepsy: A Pilot Study |
| title_full | Circulating microRNAs as Potential Novel Diagnostic Biomarkers to Predict Drug Resistance in Temporal Lobe Epilepsy: A Pilot Study |
| title_fullStr | Circulating microRNAs as Potential Novel Diagnostic Biomarkers to Predict Drug Resistance in Temporal Lobe Epilepsy: A Pilot Study |
| title_full_unstemmed | Circulating microRNAs as Potential Novel Diagnostic Biomarkers to Predict Drug Resistance in Temporal Lobe Epilepsy: A Pilot Study |
| title_short | Circulating microRNAs as Potential Novel Diagnostic Biomarkers to Predict Drug Resistance in Temporal Lobe Epilepsy: A Pilot Study |
| title_sort | circulating micrornas as potential novel diagnostic biomarkers to predict drug resistance in temporal lobe epilepsy a pilot study |
| topic | temporal lobe epilepsy miRNAs diagnosis prognosis antiseizure medications ASMs |
| url | https://www.mdpi.com/1422-0067/22/2/702 |
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