Feline Interstitial Cystitis Enhances Mucosa-Dependent Contractile Responses to Serotonin
Purpose To determine whether responses to serotonin are altered in bladder strips from cats diagnosed with a naturally occurring form of bladder pain syndrome/interstitial cystitis termed feline interstitial cystitis (FIC). Methods Full thickness bladder strips were isolated from aged matched health...
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| Format: | Article |
| Language: | English |
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Korean Continence Society
2018-12-01
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| Series: | International Neurourology Journal |
| Subjects: | |
| Online Access: | http://www.einj.org/upload/pdf/inj-1836276-138.pdf |
| _version_ | 1828355604724318208 |
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| author | Youko Ikeda Amanda Wolf-Johnston James R. Roppolo Charles A. T. Buffington Lori Birder |
| author_facet | Youko Ikeda Amanda Wolf-Johnston James R. Roppolo Charles A. T. Buffington Lori Birder |
| author_sort | Youko Ikeda |
| collection | DOAJ |
| description | Purpose To determine whether responses to serotonin are altered in bladder strips from cats diagnosed with a naturally occurring form of bladder pain syndrome/interstitial cystitis termed feline interstitial cystitis (FIC). Methods Full thickness bladder strips were isolated from aged matched healthy control cats and cats with clinically verified FIC. Bladder strips were mounted in an organ bath and connected to a tension transducer to record contractile activity. A serotonin dose response (0.01–10μM) was determined for each strip with the mucosa intact or denuded. Results Bladder strips from control and FIC cats contracted in response to serotonin in a dose-dependent manner. The normalized force of serotonin-evoked contractions was significantly greater in bladder strips from cats with FIC (n=7) than from control cats (n=4). Removal of the mucosa significantly decreased serotonin-mediated responses in both control and FIC bladder preparations. Furthermore, the contractions in response to serotonin were abolished by 1μM atropine in both control and FIC bladder strips. Conclusions The effect of serotonin on contractile force, but not sensitivity, was potentiated in bladder strips from cats with FIC, and was dependent upon the presence of the mucosa in control and FIC groups. As atropine inhibited these effects of serotonin, we hypothesize that, serotonin enhances acetylcholine release from the mucosa of FIC cat bladder strips, which could account for the increased force generated. In summary, FIC augments the responsiveness of bladder to serotonin, which may contribute to the symptoms associated with this chronic condition. |
| first_indexed | 2024-04-14T02:45:09Z |
| format | Article |
| id | doaj.art-e3e4f597cc774a8ea3964444923c4d05 |
| institution | Directory Open Access Journal |
| issn | 2093-4777 2093-6931 |
| language | English |
| last_indexed | 2024-04-14T02:45:09Z |
| publishDate | 2018-12-01 |
| publisher | Korean Continence Society |
| record_format | Article |
| series | International Neurourology Journal |
| spelling | doaj.art-e3e4f597cc774a8ea3964444923c4d052022-12-22T02:16:37ZengKorean Continence SocietyInternational Neurourology Journal2093-47772093-69312018-12-0122424625110.5213/inj.1836276.138764Feline Interstitial Cystitis Enhances Mucosa-Dependent Contractile Responses to SerotoninYouko Ikeda0Amanda Wolf-Johnston1James R. Roppolo2Charles A. T. Buffington3Lori Birder4 Division of Renal-Electrolyte, Department of Medicine, School of Medicine, University of Pittsburgh, Pittsburgh, PA, USA Division of Renal-Electrolyte, Department of Medicine, School of Medicine, University of Pittsburgh, Pittsburgh, PA, USA Department of Pharmacology and Chemical Biology, University of Pittsburgh, Pittsburgh, PA, USA Department of Veterinary Clinical Sciences, The Ohio State University, Columbus, OH, USA Division of Renal-Electrolyte, Department of Medicine, School of Medicine, University of Pittsburgh, Pittsburgh, PA, USAPurpose To determine whether responses to serotonin are altered in bladder strips from cats diagnosed with a naturally occurring form of bladder pain syndrome/interstitial cystitis termed feline interstitial cystitis (FIC). Methods Full thickness bladder strips were isolated from aged matched healthy control cats and cats with clinically verified FIC. Bladder strips were mounted in an organ bath and connected to a tension transducer to record contractile activity. A serotonin dose response (0.01–10μM) was determined for each strip with the mucosa intact or denuded. Results Bladder strips from control and FIC cats contracted in response to serotonin in a dose-dependent manner. The normalized force of serotonin-evoked contractions was significantly greater in bladder strips from cats with FIC (n=7) than from control cats (n=4). Removal of the mucosa significantly decreased serotonin-mediated responses in both control and FIC bladder preparations. Furthermore, the contractions in response to serotonin were abolished by 1μM atropine in both control and FIC bladder strips. Conclusions The effect of serotonin on contractile force, but not sensitivity, was potentiated in bladder strips from cats with FIC, and was dependent upon the presence of the mucosa in control and FIC groups. As atropine inhibited these effects of serotonin, we hypothesize that, serotonin enhances acetylcholine release from the mucosa of FIC cat bladder strips, which could account for the increased force generated. In summary, FIC augments the responsiveness of bladder to serotonin, which may contribute to the symptoms associated with this chronic condition.http://www.einj.org/upload/pdf/inj-1836276-138.pdfInterstitial cystitisSerotoninUrothelium |
| spellingShingle | Youko Ikeda Amanda Wolf-Johnston James R. Roppolo Charles A. T. Buffington Lori Birder Feline Interstitial Cystitis Enhances Mucosa-Dependent Contractile Responses to Serotonin International Neurourology Journal Interstitial cystitis Serotonin Urothelium |
| title | Feline Interstitial Cystitis Enhances Mucosa-Dependent Contractile Responses to Serotonin |
| title_full | Feline Interstitial Cystitis Enhances Mucosa-Dependent Contractile Responses to Serotonin |
| title_fullStr | Feline Interstitial Cystitis Enhances Mucosa-Dependent Contractile Responses to Serotonin |
| title_full_unstemmed | Feline Interstitial Cystitis Enhances Mucosa-Dependent Contractile Responses to Serotonin |
| title_short | Feline Interstitial Cystitis Enhances Mucosa-Dependent Contractile Responses to Serotonin |
| title_sort | feline interstitial cystitis enhances mucosa dependent contractile responses to serotonin |
| topic | Interstitial cystitis Serotonin Urothelium |
| url | http://www.einj.org/upload/pdf/inj-1836276-138.pdf |
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