Proapoptotic activity of aflatoxin B1 and sterigmatocystin in HepG2 cells
Aflatoxin B1 (AFB1) and sterigmatocystin (ST) are two hepatocarcinogenic mycotoxins that are commonly coexisted in cereal grains, and their co-proapoptotic activity in HepG2 cells was studied. The values of IC50, which is the dosage of mycotoxin resulting in a 50% cell growth inhibition measured by...
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| Format: | Article |
| Language: | English |
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Elsevier
2014-01-01
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| Series: | Toxicology Reports |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2214750014001139 |
| _version_ | 1818355428759699456 |
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| author | Yang Liu Ming Du Genyi Zhang |
| author_facet | Yang Liu Ming Du Genyi Zhang |
| author_sort | Yang Liu |
| collection | DOAJ |
| description | Aflatoxin B1 (AFB1) and sterigmatocystin (ST) are two hepatocarcinogenic mycotoxins that are commonly coexisted in cereal grains, and their co-proapoptotic activity in HepG2 cells was studied. The values of IC50, which is the dosage of mycotoxin resulting in a 50% cell growth inhibition measured by a sulforhodamine B (SRB) colorimetric assay, were 16.9 μM and 7.3 μM for AFB1 and ST, respectively. Additively and dose-dependently, cell apoptosis-related toxicity endpoints of double strand DNA and ATP content were decreased while the intracellular ROS and mitochondria membrane permeability (MMP) were increased. Consistently, when cell cycle is arrest at G0/G1 or S phase by AFB1 and/or ST, the experimental results from flow cytometry assay demonstrated that the rate of cell apoptosis and mitochondrial membrane potential were also additively increased and decreased, respectively, in a dose-dependent manner. Thus, the integrity of mitochondria (MMP and membrane potential) that is the central component of cell apoptosis is disrupted by AFB1 and ST in an additive manner. With the immunocytochemistry analysis showing increased expression of apoptosis-related proteins of Bax, Caspase-3 and p53 and decreased expression of Bcl-2 protein, an additive nature of the co-proapoptotic activity of AFB1 and ST was revealed. |
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| institution | Directory Open Access Journal |
| issn | 2214-7500 |
| language | English |
| last_indexed | 2024-12-13T19:41:10Z |
| publishDate | 2014-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Toxicology Reports |
| spelling | doaj.art-e3e8bb05ccd84894a4ce6b0bdee4a1772022-12-21T23:33:41ZengElsevierToxicology Reports2214-75002014-01-011C1076108610.1016/j.toxrep.2014.10.016Proapoptotic activity of aflatoxin B1 and sterigmatocystin in HepG2 cellsYang LiuMing DuGenyi ZhangAflatoxin B1 (AFB1) and sterigmatocystin (ST) are two hepatocarcinogenic mycotoxins that are commonly coexisted in cereal grains, and their co-proapoptotic activity in HepG2 cells was studied. The values of IC50, which is the dosage of mycotoxin resulting in a 50% cell growth inhibition measured by a sulforhodamine B (SRB) colorimetric assay, were 16.9 μM and 7.3 μM for AFB1 and ST, respectively. Additively and dose-dependently, cell apoptosis-related toxicity endpoints of double strand DNA and ATP content were decreased while the intracellular ROS and mitochondria membrane permeability (MMP) were increased. Consistently, when cell cycle is arrest at G0/G1 or S phase by AFB1 and/or ST, the experimental results from flow cytometry assay demonstrated that the rate of cell apoptosis and mitochondrial membrane potential were also additively increased and decreased, respectively, in a dose-dependent manner. Thus, the integrity of mitochondria (MMP and membrane potential) that is the central component of cell apoptosis is disrupted by AFB1 and ST in an additive manner. With the immunocytochemistry analysis showing increased expression of apoptosis-related proteins of Bax, Caspase-3 and p53 and decreased expression of Bcl-2 protein, an additive nature of the co-proapoptotic activity of AFB1 and ST was revealed.http://www.sciencedirect.com/science/article/pii/S2214750014001139Aflatoxin B1SterigmatocystinCombinative toxicityApoptosis |
| spellingShingle | Yang Liu Ming Du Genyi Zhang Proapoptotic activity of aflatoxin B1 and sterigmatocystin in HepG2 cells Toxicology Reports Aflatoxin B1 Sterigmatocystin Combinative toxicity Apoptosis |
| title | Proapoptotic activity of aflatoxin B1 and sterigmatocystin in HepG2 cells |
| title_full | Proapoptotic activity of aflatoxin B1 and sterigmatocystin in HepG2 cells |
| title_fullStr | Proapoptotic activity of aflatoxin B1 and sterigmatocystin in HepG2 cells |
| title_full_unstemmed | Proapoptotic activity of aflatoxin B1 and sterigmatocystin in HepG2 cells |
| title_short | Proapoptotic activity of aflatoxin B1 and sterigmatocystin in HepG2 cells |
| title_sort | proapoptotic activity of aflatoxin b1 and sterigmatocystin in hepg2 cells |
| topic | Aflatoxin B1 Sterigmatocystin Combinative toxicity Apoptosis |
| url | http://www.sciencedirect.com/science/article/pii/S2214750014001139 |
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