BDNF as a Putative Target for Standardized Extract of <i>Ginkgo biloba</i>-Induced Persistence of Object Recognition Memory
Despite considerable progress on the study of the effect of standardized extract of <i>Gingko biloba</i> (EGb) on memory processes, our understanding of its role in the persistence of long-term memory (LTM) and the molecular mechanism underlying its effect, particularly episodic-like mem...
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MDPI AG
2021-06-01
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| Online Access: | https://www.mdpi.com/1420-3049/26/11/3326 |
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| author | Beatriz G. Muratori Cláudia R. Zamberlam Thaís B. Mendes Bruno H. N. Nozima Janete M. Cerutti Suzete M. Cerutti |
| author_facet | Beatriz G. Muratori Cláudia R. Zamberlam Thaís B. Mendes Bruno H. N. Nozima Janete M. Cerutti Suzete M. Cerutti |
| author_sort | Beatriz G. Muratori |
| collection | DOAJ |
| description | Despite considerable progress on the study of the effect of standardized extract of <i>Gingko biloba</i> (EGb) on memory processes, our understanding of its role in the persistence of long-term memory (LTM) and the molecular mechanism underlying its effect, particularly episodic-like memory, is limited. We here investigated the effects of EGb on the long-term retention of recognition memory and its persistence and BDNF expression levels in the dorsal hippocampal formation (DHF). Adult male Wistar rats (n = 10/group) were handled for 10 min/5 day. On day 6, the animals were treated with vehicle or 0.4 mg/kg diazepam (control groups) or with EGb (250, 500 or 100 mg/kg) 30 min before the training session (TR1), in which the animals were exposed to two sample objects. On day 7, all rats underwent a second training session (TR2) as described in the TR1 but without drug treatment. Object recognition memory (ORM) was evaluated on day 8 (retention test, T1) and day 9 (persistence test, T2). At the end of T1or T2, animals were decapitated, and DHF samples were frozen at −80 °C for analyses of the differential expression of BDNF by Western blotting. EGb-treated groups spent more time exploring the novel object in T2 and showed the highest recognition index (RI) values during the T1 and T2, which was associated with upregulation of BDNF expression in the DHF in a dose-and session-dependent manner. Our data reveal, for the first time, that EGb treatment before acquisition of ORM promotes persistence of LTM by BDNF differential expression. |
| first_indexed | 2024-03-10T10:48:14Z |
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| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-10T10:48:14Z |
| publishDate | 2021-06-01 |
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| record_format | Article |
| series | Molecules |
| spelling | doaj.art-e3e92ec45e164a10b35beae8baa785562023-11-21T22:25:48ZengMDPI AGMolecules1420-30492021-06-012611332610.3390/molecules26113326BDNF as a Putative Target for Standardized Extract of <i>Ginkgo biloba</i>-Induced Persistence of Object Recognition MemoryBeatriz G. Muratori0Cláudia R. Zamberlam1Thaís B. Mendes2Bruno H. N. Nozima3Janete M. Cerutti4Suzete M. Cerutti5Cellular and Behavioral Pharmacology Laboratory, The Graduate Program in Biological Chemistry, Universidade Federal de São Paulo, São Paulo 09972-270, BrazilCellular and Behavioral Pharmacology Laboratory, The Graduate Program in Biological Chemistry, Universidade Federal de São Paulo, São Paulo 09972-270, BrazilGenetic Bases of Thyroid Tumor Laboratory, Division of Genetics, Department of Morphology and Genetics, Federal University of Sao Paulo, São Paulo 04039-032, BrazilGenetic Bases of Thyroid Tumor Laboratory, Division of Genetics, Department of Morphology and Genetics, Federal University of Sao Paulo, São Paulo 04039-032, BrazilGenetic Bases of Thyroid Tumor Laboratory, Division of Genetics, Department of Morphology and Genetics, Federal University of Sao Paulo, São Paulo 04039-032, BrazilCellular and Behavioral Pharmacology Laboratory, The Graduate Program in Biological Chemistry, Universidade Federal de São Paulo, São Paulo 09972-270, BrazilDespite considerable progress on the study of the effect of standardized extract of <i>Gingko biloba</i> (EGb) on memory processes, our understanding of its role in the persistence of long-term memory (LTM) and the molecular mechanism underlying its effect, particularly episodic-like memory, is limited. We here investigated the effects of EGb on the long-term retention of recognition memory and its persistence and BDNF expression levels in the dorsal hippocampal formation (DHF). Adult male Wistar rats (n = 10/group) were handled for 10 min/5 day. On day 6, the animals were treated with vehicle or 0.4 mg/kg diazepam (control groups) or with EGb (250, 500 or 100 mg/kg) 30 min before the training session (TR1), in which the animals were exposed to two sample objects. On day 7, all rats underwent a second training session (TR2) as described in the TR1 but without drug treatment. Object recognition memory (ORM) was evaluated on day 8 (retention test, T1) and day 9 (persistence test, T2). At the end of T1or T2, animals were decapitated, and DHF samples were frozen at −80 °C for analyses of the differential expression of BDNF by Western blotting. EGb-treated groups spent more time exploring the novel object in T2 and showed the highest recognition index (RI) values during the T1 and T2, which was associated with upregulation of BDNF expression in the DHF in a dose-and session-dependent manner. Our data reveal, for the first time, that EGb treatment before acquisition of ORM promotes persistence of LTM by BDNF differential expression.https://www.mdpi.com/1420-3049/26/11/3326memoryobject recognition<i>Ginkgo biloba</i>dorsal hippocampus formationbrain-derived neurotrophic factor |
| spellingShingle | Beatriz G. Muratori Cláudia R. Zamberlam Thaís B. Mendes Bruno H. N. Nozima Janete M. Cerutti Suzete M. Cerutti BDNF as a Putative Target for Standardized Extract of <i>Ginkgo biloba</i>-Induced Persistence of Object Recognition Memory Molecules memory object recognition <i>Ginkgo biloba</i> dorsal hippocampus formation brain-derived neurotrophic factor |
| title | BDNF as a Putative Target for Standardized Extract of <i>Ginkgo biloba</i>-Induced Persistence of Object Recognition Memory |
| title_full | BDNF as a Putative Target for Standardized Extract of <i>Ginkgo biloba</i>-Induced Persistence of Object Recognition Memory |
| title_fullStr | BDNF as a Putative Target for Standardized Extract of <i>Ginkgo biloba</i>-Induced Persistence of Object Recognition Memory |
| title_full_unstemmed | BDNF as a Putative Target for Standardized Extract of <i>Ginkgo biloba</i>-Induced Persistence of Object Recognition Memory |
| title_short | BDNF as a Putative Target for Standardized Extract of <i>Ginkgo biloba</i>-Induced Persistence of Object Recognition Memory |
| title_sort | bdnf as a putative target for standardized extract of i ginkgo biloba i induced persistence of object recognition memory |
| topic | memory object recognition <i>Ginkgo biloba</i> dorsal hippocampus formation brain-derived neurotrophic factor |
| url | https://www.mdpi.com/1420-3049/26/11/3326 |
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