In-Vitro evaluation of Isolated Staphylococcal-bacteriophage in Killing Methicillin-Resistant Staphylococcus aureus
The present study was carried out to isolate and identified the Staphylococcal-specific bacteriophage from swage water by using agar overlay method and to investigate the anti-Staphylococcal activity of هsolated Bacteriophage in-vitro. Two experiments were performed in this present study; the first...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Faculty of Veterinary Medicine, University of Kufa
2016-12-01
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| Series: | Kufa Journal for Veterinary Medical Sciences |
| Subjects: | |
| Online Access: | https://journal.uokufa.edu.iq/index.php/kjvs/article/view/4327 |
| _version_ | 1797364045706166272 |
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| author | Sarhan Rashid Sarhan Orooba Mohammed Saeed Ibrahim Serwa Ibrahim Salih |
| author_facet | Sarhan Rashid Sarhan Orooba Mohammed Saeed Ibrahim Serwa Ibrahim Salih |
| author_sort | Sarhan Rashid Sarhan |
| collection | DOAJ |
| description | The present study was carried out to isolate and identified the Staphylococcal-specific bacteriophage from swage water by using agar overlay method and to investigate the anti-Staphylococcal activity of هsolated Bacteriophage in-vitro. Two experiments were performed in this present study; the first one was isolation and identification of Methicillin-Resistant Staphylococcus aureus (MRSA) from post-traumatic bone infection. While the second experiment included study the infectivity of bacteriophage in-vitro to MRSA as well as effect of temperature and pH on the infectivity of phage was also studied. Results of antimicrobial susceptibility test of S. aureus isolates revealed that most of isolates were resistant to Methicillin, Cefoxitin and Pencillin G. Staphylococcus aureus phage was isolated from sewage samples by agar overlay method, the phage was characterized by clear, circular plaques ranged between 2-3 mm in diameter. TTA method for determined the Phage MIC appears to be both reliable, and reproducible. |
| first_indexed | 2024-03-08T16:29:43Z |
| format | Article |
| id | doaj.art-e3ea8d463413473bbc49c23860435d8e |
| institution | Directory Open Access Journal |
| issn | 2077-9798 2959-8478 |
| language | English |
| last_indexed | 2024-03-08T16:29:43Z |
| publishDate | 2016-12-01 |
| publisher | Faculty of Veterinary Medicine, University of Kufa |
| record_format | Article |
| series | Kufa Journal for Veterinary Medical Sciences |
| spelling | doaj.art-e3ea8d463413473bbc49c23860435d8e2024-01-06T10:17:27ZengFaculty of Veterinary Medicine, University of KufaKufa Journal for Veterinary Medical Sciences2077-97982959-84782016-12-017210.36326/kjvs/2016/v7i24327In-Vitro evaluation of Isolated Staphylococcal-bacteriophage in Killing Methicillin-Resistant Staphylococcus aureusSarhan Rashid SarhanOrooba Mohammed Saeed IbrahimSerwa Ibrahim SalihThe present study was carried out to isolate and identified the Staphylococcal-specific bacteriophage from swage water by using agar overlay method and to investigate the anti-Staphylococcal activity of هsolated Bacteriophage in-vitro. Two experiments were performed in this present study; the first one was isolation and identification of Methicillin-Resistant Staphylococcus aureus (MRSA) from post-traumatic bone infection. While the second experiment included study the infectivity of bacteriophage in-vitro to MRSA as well as effect of temperature and pH on the infectivity of phage was also studied. Results of antimicrobial susceptibility test of S. aureus isolates revealed that most of isolates were resistant to Methicillin, Cefoxitin and Pencillin G. Staphylococcus aureus phage was isolated from sewage samples by agar overlay method, the phage was characterized by clear, circular plaques ranged between 2-3 mm in diameter. TTA method for determined the Phage MIC appears to be both reliable, and reproducible.https://journal.uokufa.edu.iq/index.php/kjvs/article/view/4327BacteriophageBone infectionMethicillin-resistant Staphylococcus aureusTetrazolium assay |
| spellingShingle | Sarhan Rashid Sarhan Orooba Mohammed Saeed Ibrahim Serwa Ibrahim Salih In-Vitro evaluation of Isolated Staphylococcal-bacteriophage in Killing Methicillin-Resistant Staphylococcus aureus Kufa Journal for Veterinary Medical Sciences Bacteriophage Bone infection Methicillin-resistant Staphylococcus aureus Tetrazolium assay |
| title | In-Vitro evaluation of Isolated Staphylococcal-bacteriophage in Killing Methicillin-Resistant Staphylococcus aureus |
| title_full | In-Vitro evaluation of Isolated Staphylococcal-bacteriophage in Killing Methicillin-Resistant Staphylococcus aureus |
| title_fullStr | In-Vitro evaluation of Isolated Staphylococcal-bacteriophage in Killing Methicillin-Resistant Staphylococcus aureus |
| title_full_unstemmed | In-Vitro evaluation of Isolated Staphylococcal-bacteriophage in Killing Methicillin-Resistant Staphylococcus aureus |
| title_short | In-Vitro evaluation of Isolated Staphylococcal-bacteriophage in Killing Methicillin-Resistant Staphylococcus aureus |
| title_sort | in vitro evaluation of isolated staphylococcal bacteriophage in killing methicillin resistant staphylococcus aureus |
| topic | Bacteriophage Bone infection Methicillin-resistant Staphylococcus aureus Tetrazolium assay |
| url | https://journal.uokufa.edu.iq/index.php/kjvs/article/view/4327 |
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