Differential Induction Pattern Towards Classically Activated Macrophages in Response to an Immunomodulatory Extract from <i>Pleurotus ostreatus</i> Mycelium
<i>Pleurotus ostreatus</i> mushroom preparations have been investigated because of their ability to modulate the immune function. However, there is still no consensus regarding the activation and polarizing effect on macrophages by <i>Pleurotus</i>-derived bioproducts. This s...
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| Format: | Article |
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MDPI AG
2021-03-01
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| Series: | Journal of Fungi |
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| Online Access: | https://www.mdpi.com/2309-608X/7/3/206 |
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| author | Gabriel Llauradó Maury Humberto J. Morris-Quevedo Annick Heykers Ellen Lanckacker Davie Cappoen Peter Delputte Wim Vanden Berghe Zelene Salgueiro Paul Cos |
| author_facet | Gabriel Llauradó Maury Humberto J. Morris-Quevedo Annick Heykers Ellen Lanckacker Davie Cappoen Peter Delputte Wim Vanden Berghe Zelene Salgueiro Paul Cos |
| author_sort | Gabriel Llauradó Maury |
| collection | DOAJ |
| description | <i>Pleurotus ostreatus</i> mushroom preparations have been investigated because of their ability to modulate the immune function. However, there is still no consensus regarding the activation and polarizing effect on macrophages by <i>Pleurotus</i>-derived bioproducts. This study examined the immune-activating effect of a mycelium-derived <i>P. ostreatus</i> aqueous extract (HW-Pm) on macrophage functions, by means of the determination of nitric oxide (NO) production, the mRNA expression of inducible nitric oxide synthase (iNOS), Arginase-1 and FIZZ and the cytokine levels. The phagocytic activity and the activation of NF-κB in U937 reporter cells were also investigated. No cytotoxicity was observed in macrophages treated with HW-Pm (IC<sub>50</sub> > 1024 μg/mL) by the resazurin test. HW-Pm induced high levels of NO production and iNOS expression in macrophages. In contrast, HW-Pm did not induce Arginase-1 and FIZZ mRNA expressions. The mushroom extract increased TNF-α and IL-6 production and the phagocytic function in murine macrophages. It also stimulated the activation of the NF-κB promoter. The <i>P. ostreatus</i> mycelium extract has a potential application as a natural immune-enhancing agent, by targeting macrophage activation towards the classically activated subset and stimulating macrophage-mediated innate immune responses. |
| first_indexed | 2024-03-10T13:20:45Z |
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| id | doaj.art-e3ed9ff613ae4b36b0b8e23e2987dcd7 |
| institution | Directory Open Access Journal |
| issn | 2309-608X |
| language | English |
| last_indexed | 2024-03-10T13:20:45Z |
| publishDate | 2021-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Journal of Fungi |
| spelling | doaj.art-e3ed9ff613ae4b36b0b8e23e2987dcd72023-11-21T10:06:37ZengMDPI AGJournal of Fungi2309-608X2021-03-017320610.3390/jof7030206Differential Induction Pattern Towards Classically Activated Macrophages in Response to an Immunomodulatory Extract from <i>Pleurotus ostreatus</i> MyceliumGabriel Llauradó Maury0Humberto J. Morris-Quevedo1Annick Heykers2Ellen Lanckacker3Davie Cappoen4Peter Delputte5Wim Vanden Berghe6Zelene Salgueiro7Paul Cos8Centre of Studies for Industrial Biotechnology (CEBI), University of Oriente, Ave. Patricio Lumumba s/n, Reparto Jiménez, Santiago de Cuba 90500, CubaCentre of Studies for Industrial Biotechnology (CEBI), University of Oriente, Ave. Patricio Lumumba s/n, Reparto Jiménez, Santiago de Cuba 90500, CubaLaboratory for Microbiology, Parasitology and Hygiene (LMPH), Department of Pharmaceutical Science, Faculty of Pharmaceutical, Biomedical and Veterinary Sciences, University of Antwerp, Universiteitsplein 1, 2610 Antwerp, BelgiumLaboratory for Microbiology, Parasitology and Hygiene (LMPH), Department of Pharmaceutical Science, Faculty of Pharmaceutical, Biomedical and Veterinary Sciences, University of Antwerp, Universiteitsplein 1, 2610 Antwerp, BelgiumLaboratory for Microbiology, Parasitology and Hygiene (LMPH), Department of Pharmaceutical Science, Faculty of Pharmaceutical, Biomedical and Veterinary Sciences, University of Antwerp, Universiteitsplein 1, 2610 Antwerp, BelgiumLaboratory for Microbiology, Parasitology and Hygiene (LMPH), Department of Pharmaceutical Science, Faculty of Pharmaceutical, Biomedical and Veterinary Sciences, University of Antwerp, Universiteitsplein 1, 2610 Antwerp, BelgiumLaboratory of Protein Chemistry, Proteomics and Epigenetics Signalling (PPES), Department of Biomedical Sciences, Faculty of Pharmaceutical, Biomedical and Veterinary Sciences, University of Antwerp, Universiteitsplein 1, 2610 Antwerp, BelgiumPharmaceutics Laboratories, BioCubaFarma, Ave 5ta, Distrito 30 de Noviembre, Santiago de Cuba 90400, CubaLaboratory for Microbiology, Parasitology and Hygiene (LMPH), Department of Pharmaceutical Science, Faculty of Pharmaceutical, Biomedical and Veterinary Sciences, University of Antwerp, Universiteitsplein 1, 2610 Antwerp, Belgium<i>Pleurotus ostreatus</i> mushroom preparations have been investigated because of their ability to modulate the immune function. However, there is still no consensus regarding the activation and polarizing effect on macrophages by <i>Pleurotus</i>-derived bioproducts. This study examined the immune-activating effect of a mycelium-derived <i>P. ostreatus</i> aqueous extract (HW-Pm) on macrophage functions, by means of the determination of nitric oxide (NO) production, the mRNA expression of inducible nitric oxide synthase (iNOS), Arginase-1 and FIZZ and the cytokine levels. The phagocytic activity and the activation of NF-κB in U937 reporter cells were also investigated. No cytotoxicity was observed in macrophages treated with HW-Pm (IC<sub>50</sub> > 1024 μg/mL) by the resazurin test. HW-Pm induced high levels of NO production and iNOS expression in macrophages. In contrast, HW-Pm did not induce Arginase-1 and FIZZ mRNA expressions. The mushroom extract increased TNF-α and IL-6 production and the phagocytic function in murine macrophages. It also stimulated the activation of the NF-κB promoter. The <i>P. ostreatus</i> mycelium extract has a potential application as a natural immune-enhancing agent, by targeting macrophage activation towards the classically activated subset and stimulating macrophage-mediated innate immune responses.https://www.mdpi.com/2309-608X/7/3/206immune-activatingmacrophagemushroommycelium<i>Pleurotus ostreatus</i> |
| spellingShingle | Gabriel Llauradó Maury Humberto J. Morris-Quevedo Annick Heykers Ellen Lanckacker Davie Cappoen Peter Delputte Wim Vanden Berghe Zelene Salgueiro Paul Cos Differential Induction Pattern Towards Classically Activated Macrophages in Response to an Immunomodulatory Extract from <i>Pleurotus ostreatus</i> Mycelium Journal of Fungi immune-activating macrophage mushroom mycelium <i>Pleurotus ostreatus</i> |
| title | Differential Induction Pattern Towards Classically Activated Macrophages in Response to an Immunomodulatory Extract from <i>Pleurotus ostreatus</i> Mycelium |
| title_full | Differential Induction Pattern Towards Classically Activated Macrophages in Response to an Immunomodulatory Extract from <i>Pleurotus ostreatus</i> Mycelium |
| title_fullStr | Differential Induction Pattern Towards Classically Activated Macrophages in Response to an Immunomodulatory Extract from <i>Pleurotus ostreatus</i> Mycelium |
| title_full_unstemmed | Differential Induction Pattern Towards Classically Activated Macrophages in Response to an Immunomodulatory Extract from <i>Pleurotus ostreatus</i> Mycelium |
| title_short | Differential Induction Pattern Towards Classically Activated Macrophages in Response to an Immunomodulatory Extract from <i>Pleurotus ostreatus</i> Mycelium |
| title_sort | differential induction pattern towards classically activated macrophages in response to an immunomodulatory extract from i pleurotus ostreatus i mycelium |
| topic | immune-activating macrophage mushroom mycelium <i>Pleurotus ostreatus</i> |
| url | https://www.mdpi.com/2309-608X/7/3/206 |
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