Comparative analysis of KNOX genes and their expression patterns under various treatments in Dendrobium huoshanense
IntroductionKNOX plays a pivotal role in governing plant growth, development, and responses to diverse abiotic and biotic stresses. However, information on the relationship between the KNOX gene family and expression levels under different treatments in Dendrobium is still limited.MethodsTo address...
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Frontiers Media S.A.
2023-10-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2023.1258533/full |
| _version_ | 1797666545923522560 |
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| author | Guohui Li Muhammad Aamir Manzoor Guoyu Wang Cunwu Chen Cheng Song |
| author_facet | Guohui Li Muhammad Aamir Manzoor Guoyu Wang Cunwu Chen Cheng Song |
| author_sort | Guohui Li |
| collection | DOAJ |
| description | IntroductionKNOX plays a pivotal role in governing plant growth, development, and responses to diverse abiotic and biotic stresses. However, information on the relationship between the KNOX gene family and expression levels under different treatments in Dendrobium is still limited.MethodsTo address this problem, we first used bioinformatics methods and revealed the presence of 19 KNOX genes distributed among 13 chromosomes in the Dendrobium huoshanense genome. Through an analysis of phylogenetic relationships, these genes were classified into three distinct clades: class I, class II, and class M. Our investigation included promoter analysis, revealing various cis-acting elements associated with hormones, growth and development, and abiotic stress responses. Additionally, qRT-PCR experiments were conducted to assess the expression patterns of DhKNOX genes under different treatments, including ABA, MeJA, SA, and drought.ResultsThe results demonstrated differential expression of DhKNOX genes in response to these treatments, thereby highlighting their potential roles in stress adaptation.DiscussionOverall, our results contribute important insights for further investigations into the functional characterization of the Dendrobium KNOX gene family, shedding light on their roles in plant development and stress responses. |
| first_indexed | 2024-03-11T19:59:56Z |
| format | Article |
| id | doaj.art-e3f3954f57ae4d1587f9443f459158df |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-03-11T19:59:56Z |
| publishDate | 2023-10-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-e3f3954f57ae4d1587f9443f459158df2023-10-04T09:51:25ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2023-10-011410.3389/fpls.2023.12585331258533Comparative analysis of KNOX genes and their expression patterns under various treatments in Dendrobium huoshanenseGuohui Li0Muhammad Aamir Manzoor1Guoyu Wang2Cunwu Chen3Cheng Song4Anhui Engineering Research Center for Eco-agriculture of Traditional Chinese Medicine, Anhui Dabieshan Academy of Traditional Chinese Medicine, College of Biological and Pharmaceutical Engineering, West Anhui University, Lu’an, ChinaDepartment of Plant Science, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, ChinaCollege of pharmacy, Anhui University of Chinese Medicine, Hefei, ChinaAnhui Engineering Research Center for Eco-agriculture of Traditional Chinese Medicine, Anhui Dabieshan Academy of Traditional Chinese Medicine, College of Biological and Pharmaceutical Engineering, West Anhui University, Lu’an, ChinaAnhui Engineering Research Center for Eco-agriculture of Traditional Chinese Medicine, Anhui Dabieshan Academy of Traditional Chinese Medicine, College of Biological and Pharmaceutical Engineering, West Anhui University, Lu’an, ChinaIntroductionKNOX plays a pivotal role in governing plant growth, development, and responses to diverse abiotic and biotic stresses. However, information on the relationship between the KNOX gene family and expression levels under different treatments in Dendrobium is still limited.MethodsTo address this problem, we first used bioinformatics methods and revealed the presence of 19 KNOX genes distributed among 13 chromosomes in the Dendrobium huoshanense genome. Through an analysis of phylogenetic relationships, these genes were classified into three distinct clades: class I, class II, and class M. Our investigation included promoter analysis, revealing various cis-acting elements associated with hormones, growth and development, and abiotic stress responses. Additionally, qRT-PCR experiments were conducted to assess the expression patterns of DhKNOX genes under different treatments, including ABA, MeJA, SA, and drought.ResultsThe results demonstrated differential expression of DhKNOX genes in response to these treatments, thereby highlighting their potential roles in stress adaptation.DiscussionOverall, our results contribute important insights for further investigations into the functional characterization of the Dendrobium KNOX gene family, shedding light on their roles in plant development and stress responses.https://www.frontiersin.org/articles/10.3389/fpls.2023.1258533/fullKNOXabiotic stressgene expressionD. huoshanensegenome |
| spellingShingle | Guohui Li Muhammad Aamir Manzoor Guoyu Wang Cunwu Chen Cheng Song Comparative analysis of KNOX genes and their expression patterns under various treatments in Dendrobium huoshanense Frontiers in Plant Science KNOX abiotic stress gene expression D. huoshanense genome |
| title | Comparative analysis of KNOX genes and their expression patterns under various treatments in Dendrobium huoshanense |
| title_full | Comparative analysis of KNOX genes and their expression patterns under various treatments in Dendrobium huoshanense |
| title_fullStr | Comparative analysis of KNOX genes and their expression patterns under various treatments in Dendrobium huoshanense |
| title_full_unstemmed | Comparative analysis of KNOX genes and their expression patterns under various treatments in Dendrobium huoshanense |
| title_short | Comparative analysis of KNOX genes and their expression patterns under various treatments in Dendrobium huoshanense |
| title_sort | comparative analysis of knox genes and their expression patterns under various treatments in dendrobium huoshanense |
| topic | KNOX abiotic stress gene expression D. huoshanense genome |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2023.1258533/full |
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