Understanding of Ovarian Cancer Cell-Derived Exosome Tropism for Future Therapeutic Applications
Exosomes, a subtype of extracellular vesicles, ranging from 50 to 200 nm in diameter, and mediate cell-to-cell communication in normal biological and pathological processes. Exosomes derived from tumors have multiple functions in cancer progression, resistance, and metastasis through cancer exosome-...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-05-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/24/9/8166 |
| _version_ | 1797602479646441472 |
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| author | Xiaoyu Ren Changsun Kang Lucila Garcia-Contreras Dongin Kim |
| author_facet | Xiaoyu Ren Changsun Kang Lucila Garcia-Contreras Dongin Kim |
| author_sort | Xiaoyu Ren |
| collection | DOAJ |
| description | Exosomes, a subtype of extracellular vesicles, ranging from 50 to 200 nm in diameter, and mediate cell-to-cell communication in normal biological and pathological processes. Exosomes derived from tumors have multiple functions in cancer progression, resistance, and metastasis through cancer exosome-derived tropism. However, there is no quantitative information on cancer exosome-derived tropism. Such data would be highly beneficial to guide cancer therapy by inhibiting exosome release and/or uptake. Using two fluorescent protein (mKate2) transfected ovarian cancer cell lines (OVCA4 and OVCA8), cancer exosome tropism was quantified by measuring the released exosome from ovarian cancer cells and determining the uptake of exosomes into parental ovarian cancer cells, 3D spheroids, and tumors in tumor-bearing mice. The OVCA4 cells release 50 to 200 exosomes per cell, and the OVCA8 cells do 300 to 560 per cell. The uptake of exosomes by parental ovarian cancer cells is many-fold higher than by non-parental cells. In tumor-bearing mice, most exosomes are homing to the parent cancer rather than other tissues. We successfully quantified exosome release and uptake by the parent cancer cells, further proving the tropism of cancer cell-derived exosomes. The results implied that cancer exosome tropism could provide useful information for future cancer therapeutic applications. |
| first_indexed | 2024-03-11T04:16:37Z |
| format | Article |
| id | doaj.art-e3f40d3a877e446f8234da8ced7be3c7 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-11T04:16:37Z |
| publishDate | 2023-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-e3f40d3a877e446f8234da8ced7be3c72023-11-17T23:05:50ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672023-05-01249816610.3390/ijms24098166Understanding of Ovarian Cancer Cell-Derived Exosome Tropism for Future Therapeutic ApplicationsXiaoyu Ren0Changsun Kang1Lucila Garcia-Contreras2Dongin Kim3Department of Pharmaceutical Sciences, College of Pharmacy, University of Oklahoma Health Sciences Center, Oklahoma City, OK 73117, USADepartment of Pharmaceutical Sciences, College of Pharmacy, University of Oklahoma Health Sciences Center, Oklahoma City, OK 73117, USADepartment of Pharmaceutical Sciences, College of Pharmacy, University of Oklahoma Health Sciences Center, Oklahoma City, OK 73117, USADepartment of Pharmaceutical Sciences, College of Pharmacy, University of Oklahoma Health Sciences Center, Oklahoma City, OK 73117, USAExosomes, a subtype of extracellular vesicles, ranging from 50 to 200 nm in diameter, and mediate cell-to-cell communication in normal biological and pathological processes. Exosomes derived from tumors have multiple functions in cancer progression, resistance, and metastasis through cancer exosome-derived tropism. However, there is no quantitative information on cancer exosome-derived tropism. Such data would be highly beneficial to guide cancer therapy by inhibiting exosome release and/or uptake. Using two fluorescent protein (mKate2) transfected ovarian cancer cell lines (OVCA4 and OVCA8), cancer exosome tropism was quantified by measuring the released exosome from ovarian cancer cells and determining the uptake of exosomes into parental ovarian cancer cells, 3D spheroids, and tumors in tumor-bearing mice. The OVCA4 cells release 50 to 200 exosomes per cell, and the OVCA8 cells do 300 to 560 per cell. The uptake of exosomes by parental ovarian cancer cells is many-fold higher than by non-parental cells. In tumor-bearing mice, most exosomes are homing to the parent cancer rather than other tissues. We successfully quantified exosome release and uptake by the parent cancer cells, further proving the tropism of cancer cell-derived exosomes. The results implied that cancer exosome tropism could provide useful information for future cancer therapeutic applications.https://www.mdpi.com/1422-0067/24/9/8166exosomequantificationtropismovarian cancer |
| spellingShingle | Xiaoyu Ren Changsun Kang Lucila Garcia-Contreras Dongin Kim Understanding of Ovarian Cancer Cell-Derived Exosome Tropism for Future Therapeutic Applications International Journal of Molecular Sciences exosome quantification tropism ovarian cancer |
| title | Understanding of Ovarian Cancer Cell-Derived Exosome Tropism for Future Therapeutic Applications |
| title_full | Understanding of Ovarian Cancer Cell-Derived Exosome Tropism for Future Therapeutic Applications |
| title_fullStr | Understanding of Ovarian Cancer Cell-Derived Exosome Tropism for Future Therapeutic Applications |
| title_full_unstemmed | Understanding of Ovarian Cancer Cell-Derived Exosome Tropism for Future Therapeutic Applications |
| title_short | Understanding of Ovarian Cancer Cell-Derived Exosome Tropism for Future Therapeutic Applications |
| title_sort | understanding of ovarian cancer cell derived exosome tropism for future therapeutic applications |
| topic | exosome quantification tropism ovarian cancer |
| url | https://www.mdpi.com/1422-0067/24/9/8166 |
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