Enhanced Chlorophyll Degradation Triggers the Pod Degreening of “Golden Hook,” a Special Ecotype in Common Bean (Phaseolus vulgaris L.)
To reveal genetic factors or pathways involved in the pod degreening, we performed transcriptome and metabolome analyses using a yellow pod cultivar of the common bean “golden hook” ecotype and its green pod mutants yielded via gamma radiation. Transcriptional profiling showed that expression levels...
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Frontiers Media S.A.
2020-10-01
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| Series: | Frontiers in Genetics |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fgene.2020.570816/full |
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| author | Bo Hu Bo Hu Jinlong Zhu Hongyan Wu Kun Xu Hong Zhai Ning Guo Yi Gao Yi Gao Jiayin Yang Danhua Zhu Zhengjun Xia |
| author_facet | Bo Hu Bo Hu Jinlong Zhu Hongyan Wu Kun Xu Hong Zhai Ning Guo Yi Gao Yi Gao Jiayin Yang Danhua Zhu Zhengjun Xia |
| author_sort | Bo Hu |
| collection | DOAJ |
| description | To reveal genetic factors or pathways involved in the pod degreening, we performed transcriptome and metabolome analyses using a yellow pod cultivar of the common bean “golden hook” ecotype and its green pod mutants yielded via gamma radiation. Transcriptional profiling showed that expression levels of red chlorophyll catabolite reductase (RCCR, Phvul.008G280300) involved in chlorophyll degradation was strongly enhanced at an early stage (2 cm long) in wild type but not in green pod mutants. The expression levels of genes involved in cellulose synthesis was inhibited by the pod degreening. Metabolomic profiling showed that the content of most flavonoid, flavones, and isoflavonoid was decreased during pod development, but the content of afzelechin, taxifolin, dihydrokaempferol, and cyanidin 3-O-rutinoside was remarkably increased in both wild type and green pod mutant. This study revealed that the pod degreening of the golden hook resulting from chlorophyll degradation could trigger changes in cellulose and flavonoids biosynthesis pathway, offering this cultivar a special color appearance and good flavor. |
| first_indexed | 2024-12-13T04:12:14Z |
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| id | doaj.art-e3f76d23ad8a4cf79cb75f5da6e6ea0d |
| institution | Directory Open Access Journal |
| issn | 1664-8021 |
| language | English |
| last_indexed | 2024-12-13T04:12:14Z |
| publishDate | 2020-10-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Genetics |
| spelling | doaj.art-e3f76d23ad8a4cf79cb75f5da6e6ea0d2022-12-22T00:00:02ZengFrontiers Media S.A.Frontiers in Genetics1664-80212020-10-011110.3389/fgene.2020.570816570816Enhanced Chlorophyll Degradation Triggers the Pod Degreening of “Golden Hook,” a Special Ecotype in Common Bean (Phaseolus vulgaris L.)Bo Hu0Bo Hu1Jinlong Zhu2Hongyan Wu3Kun Xu4Hong Zhai5Ning Guo6Yi Gao7Yi Gao8Jiayin Yang9Danhua Zhu10Zhengjun Xia11Key Laboratory of Soybean Molecular Design Breeding, Northeast Institute of Geography and Agroecology, Chinese Academy of Sciences, Harbin, ChinaUniversity of Chinese Academy of Sciences, Beijing, ChinaKey Laboratory of Soybean Molecular Design Breeding, Northeast Institute of Geography and Agroecology, Chinese Academy of Sciences, Harbin, ChinaKey Laboratory of Soybean Molecular Design Breeding, Northeast Institute of Geography and Agroecology, Chinese Academy of Sciences, Harbin, ChinaKey Laboratory of Soybean Molecular Design Breeding, Northeast Institute of Geography and Agroecology, Chinese Academy of Sciences, Harbin, ChinaKey Laboratory of Soybean Molecular Design Breeding, Northeast Institute of Geography and Agroecology, Chinese Academy of Sciences, Harbin, ChinaKey Laboratory of Soybean Molecular Design Breeding, Northeast Institute of Geography and Agroecology, Chinese Academy of Sciences, Harbin, ChinaKey Laboratory of Soybean Molecular Design Breeding, Northeast Institute of Geography and Agroecology, Chinese Academy of Sciences, Harbin, ChinaUniversity of Chinese Academy of Sciences, Beijing, ChinaHuaiyin Institute of Agricultural Science of Xuhuai Region, Huai’an, ChinaZhejiang Academy of Agricultural Sciences, Hangzhou, ChinaKey Laboratory of Soybean Molecular Design Breeding, Northeast Institute of Geography and Agroecology, Chinese Academy of Sciences, Harbin, ChinaTo reveal genetic factors or pathways involved in the pod degreening, we performed transcriptome and metabolome analyses using a yellow pod cultivar of the common bean “golden hook” ecotype and its green pod mutants yielded via gamma radiation. Transcriptional profiling showed that expression levels of red chlorophyll catabolite reductase (RCCR, Phvul.008G280300) involved in chlorophyll degradation was strongly enhanced at an early stage (2 cm long) in wild type but not in green pod mutants. The expression levels of genes involved in cellulose synthesis was inhibited by the pod degreening. Metabolomic profiling showed that the content of most flavonoid, flavones, and isoflavonoid was decreased during pod development, but the content of afzelechin, taxifolin, dihydrokaempferol, and cyanidin 3-O-rutinoside was remarkably increased in both wild type and green pod mutant. This study revealed that the pod degreening of the golden hook resulting from chlorophyll degradation could trigger changes in cellulose and flavonoids biosynthesis pathway, offering this cultivar a special color appearance and good flavor.https://www.frontiersin.org/article/10.3389/fgene.2020.570816/fullcommon bean (Phaseolus vulgaris L.)degreeningpodtranscriptomecellulose |
| spellingShingle | Bo Hu Bo Hu Jinlong Zhu Hongyan Wu Kun Xu Hong Zhai Ning Guo Yi Gao Yi Gao Jiayin Yang Danhua Zhu Zhengjun Xia Enhanced Chlorophyll Degradation Triggers the Pod Degreening of “Golden Hook,” a Special Ecotype in Common Bean (Phaseolus vulgaris L.) Frontiers in Genetics common bean (Phaseolus vulgaris L.) degreening pod transcriptome cellulose |
| title | Enhanced Chlorophyll Degradation Triggers the Pod Degreening of “Golden Hook,” a Special Ecotype in Common Bean (Phaseolus vulgaris L.) |
| title_full | Enhanced Chlorophyll Degradation Triggers the Pod Degreening of “Golden Hook,” a Special Ecotype in Common Bean (Phaseolus vulgaris L.) |
| title_fullStr | Enhanced Chlorophyll Degradation Triggers the Pod Degreening of “Golden Hook,” a Special Ecotype in Common Bean (Phaseolus vulgaris L.) |
| title_full_unstemmed | Enhanced Chlorophyll Degradation Triggers the Pod Degreening of “Golden Hook,” a Special Ecotype in Common Bean (Phaseolus vulgaris L.) |
| title_short | Enhanced Chlorophyll Degradation Triggers the Pod Degreening of “Golden Hook,” a Special Ecotype in Common Bean (Phaseolus vulgaris L.) |
| title_sort | enhanced chlorophyll degradation triggers the pod degreening of golden hook a special ecotype in common bean phaseolus vulgaris l |
| topic | common bean (Phaseolus vulgaris L.) degreening pod transcriptome cellulose |
| url | https://www.frontiersin.org/article/10.3389/fgene.2020.570816/full |
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