Synthesis and structural characteristics analysis of melanin pigments induced by blue light in Morchella sextelata
Morchella sextelata, a highly sought-after edible mushroom worldwide, is evaluated based on its cap color as an essential commercial property indicator. In the present study, the effects of blue light on cap pigmentation in M. sextelata, as well as the synthesis and structural characteristics of mel...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-09-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2023.1276457/full |
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| author | Zhiheng Qiu Zhiheng Qiu Shuang Wang Shuang Wang Jiazhi Zhao Jiazhi Zhao Lingxiu Cui Lingxiu Cui Xinyi Wang Xinyi Wang Nuo Cai Nuo Cai Hongpeng Li Hongpeng Li Shuhua Ren Shuhua Ren Tianlai Li Tianlai Li Lili Shu Lili Shu |
| author_facet | Zhiheng Qiu Zhiheng Qiu Shuang Wang Shuang Wang Jiazhi Zhao Jiazhi Zhao Lingxiu Cui Lingxiu Cui Xinyi Wang Xinyi Wang Nuo Cai Nuo Cai Hongpeng Li Hongpeng Li Shuhua Ren Shuhua Ren Tianlai Li Tianlai Li Lili Shu Lili Shu |
| author_sort | Zhiheng Qiu |
| collection | DOAJ |
| description | Morchella sextelata, a highly sought-after edible mushroom worldwide, is evaluated based on its cap color as an essential commercial property indicator. In the present study, the effects of blue light on cap pigmentation in M. sextelata, as well as the synthesis and structural characteristics of melanin pigments within the cap were examined. The results showed that an increase in the proportion of blue light within the lighting environment promoted melanin synthesis and melanization of the cap. Transmission and scanning electron microscopy revealed the localization of melanin within the mycelium and its ultrastructural characteristics. The UV–visible analysis demonstrated that melanin exhibited a maximum absorption peak at 220 nm and possessed high alkaline solubility as well as acid precipitability. The structural characteristics of melanin were analyzed using FTIR, NMR, HPLC, and elemental analysis, which confirmed the presence of eumelanin, pheomelanin, and allomelanin in both brown and black caps. Furthermore, blue light can stimulate the synthesis of both eumelanin and pheomelanin. The obtained results can serve as the foundation for comprehending the mechanism by which light regulates color formation in mushrooms. |
| first_indexed | 2024-03-11T20:41:50Z |
| format | Article |
| id | doaj.art-e3f872c0fcd4465fa2233d201ccd3074 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-03-11T20:41:50Z |
| publishDate | 2023-09-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-e3f872c0fcd4465fa2233d201ccd30742023-10-02T02:16:23ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2023-09-011410.3389/fmicb.2023.12764571276457Synthesis and structural characteristics analysis of melanin pigments induced by blue light in Morchella sextelataZhiheng Qiu0Zhiheng Qiu1Shuang Wang2Shuang Wang3Jiazhi Zhao4Jiazhi Zhao5Lingxiu Cui6Lingxiu Cui7Xinyi Wang8Xinyi Wang9Nuo Cai10Nuo Cai11Hongpeng Li12Hongpeng Li13Shuhua Ren14Shuhua Ren15Tianlai Li16Tianlai Li17Lili Shu18Lili Shu19Modern Protected Horticulture Engineering & Technology Center, College of Horticulture, Shenyang Agricultural University, Shenyang, ChinaKey Laboratory of Protected Horticulture of Education Ministry and Liaoning Province, Shenyang, ChinaModern Protected Horticulture Engineering & Technology Center, College of Horticulture, Shenyang Agricultural University, Shenyang, ChinaKey Laboratory of Protected Horticulture of Education Ministry and Liaoning Province, Shenyang, ChinaModern Protected Horticulture Engineering & Technology Center, College of Horticulture, Shenyang Agricultural University, Shenyang, ChinaKey Laboratory of Protected Horticulture of Education Ministry and Liaoning Province, Shenyang, ChinaModern Protected Horticulture Engineering & Technology Center, College of Horticulture, Shenyang Agricultural University, Shenyang, ChinaKey Laboratory of Protected Horticulture of Education Ministry and Liaoning Province, Shenyang, ChinaModern Protected Horticulture Engineering & Technology Center, College of Horticulture, Shenyang Agricultural University, Shenyang, ChinaKey Laboratory of Protected Horticulture of Education Ministry and Liaoning Province, Shenyang, ChinaModern Protected Horticulture Engineering & Technology Center, College of Horticulture, Shenyang Agricultural University, Shenyang, ChinaKey Laboratory of Protected Horticulture of Education Ministry and Liaoning Province, Shenyang, ChinaModern Protected Horticulture Engineering & Technology Center, College of Horticulture, Shenyang Agricultural University, Shenyang, ChinaKey Laboratory of Protected Horticulture of Education Ministry and Liaoning Province, Shenyang, ChinaModern Protected Horticulture Engineering & Technology Center, College of Horticulture, Shenyang Agricultural University, Shenyang, ChinaKey Laboratory of Protected Horticulture of Education Ministry and Liaoning Province, Shenyang, ChinaModern Protected Horticulture Engineering & Technology Center, College of Horticulture, Shenyang Agricultural University, Shenyang, ChinaKey Laboratory of Protected Horticulture of Education Ministry and Liaoning Province, Shenyang, ChinaModern Protected Horticulture Engineering & Technology Center, College of Horticulture, Shenyang Agricultural University, Shenyang, ChinaKey Laboratory of Protected Horticulture of Education Ministry and Liaoning Province, Shenyang, ChinaMorchella sextelata, a highly sought-after edible mushroom worldwide, is evaluated based on its cap color as an essential commercial property indicator. In the present study, the effects of blue light on cap pigmentation in M. sextelata, as well as the synthesis and structural characteristics of melanin pigments within the cap were examined. The results showed that an increase in the proportion of blue light within the lighting environment promoted melanin synthesis and melanization of the cap. Transmission and scanning electron microscopy revealed the localization of melanin within the mycelium and its ultrastructural characteristics. The UV–visible analysis demonstrated that melanin exhibited a maximum absorption peak at 220 nm and possessed high alkaline solubility as well as acid precipitability. The structural characteristics of melanin were analyzed using FTIR, NMR, HPLC, and elemental analysis, which confirmed the presence of eumelanin, pheomelanin, and allomelanin in both brown and black caps. Furthermore, blue light can stimulate the synthesis of both eumelanin and pheomelanin. The obtained results can serve as the foundation for comprehending the mechanism by which light regulates color formation in mushrooms.https://www.frontiersin.org/articles/10.3389/fmicb.2023.1276457/fullMorchella sextelatablue lightmelaninsynthesisultrastructural characteristicscolor formation |
| spellingShingle | Zhiheng Qiu Zhiheng Qiu Shuang Wang Shuang Wang Jiazhi Zhao Jiazhi Zhao Lingxiu Cui Lingxiu Cui Xinyi Wang Xinyi Wang Nuo Cai Nuo Cai Hongpeng Li Hongpeng Li Shuhua Ren Shuhua Ren Tianlai Li Tianlai Li Lili Shu Lili Shu Synthesis and structural characteristics analysis of melanin pigments induced by blue light in Morchella sextelata Frontiers in Microbiology Morchella sextelata blue light melanin synthesis ultrastructural characteristics color formation |
| title | Synthesis and structural characteristics analysis of melanin pigments induced by blue light in Morchella sextelata |
| title_full | Synthesis and structural characteristics analysis of melanin pigments induced by blue light in Morchella sextelata |
| title_fullStr | Synthesis and structural characteristics analysis of melanin pigments induced by blue light in Morchella sextelata |
| title_full_unstemmed | Synthesis and structural characteristics analysis of melanin pigments induced by blue light in Morchella sextelata |
| title_short | Synthesis and structural characteristics analysis of melanin pigments induced by blue light in Morchella sextelata |
| title_sort | synthesis and structural characteristics analysis of melanin pigments induced by blue light in morchella sextelata |
| topic | Morchella sextelata blue light melanin synthesis ultrastructural characteristics color formation |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2023.1276457/full |
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