Strategically examining the full-genome of dengue virus type 3 in clinical isolates reveals its mutation spectra
<p>Abstract</p> <p>Background</p> <p>Previous studies presented the quasispecies spectrum of the envelope region of dengue virus type 3 (DENV-3) from either clinical specimens or field-caught mosquitoes. However, the extent of sequence variation among full genomic seque...
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| Format: | Article |
| Language: | English |
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BMC
2005-08-01
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| Series: | Virology Journal |
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| Online Access: | http://www.virologyj.com/content/2/1/72 |
| _version_ | 1818155081144467456 |
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| author | Wu Hui-Lin Chen Wei-June Wang Wei-Kung King Chwan-Chuen Chao Day-Yu Chang Gwong-Jen J |
| author_facet | Wu Hui-Lin Chen Wei-June Wang Wei-Kung King Chwan-Chuen Chao Day-Yu Chang Gwong-Jen J |
| author_sort | Wu Hui-Lin |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>Previous studies presented the quasispecies spectrum of the envelope region of dengue virus type 3 (DENV-3) from either clinical specimens or field-caught mosquitoes. However, the extent of sequence variation among full genomic sequences of DENV within infected individuals remains largely unknown.</p> <p>Results</p> <p>Instead of arbitrarily choosing one genomic region in this study, the full genomic consensus sequences of six DENV-3 isolates were used to locate four genomic regions that had a higher potential of sequence heterogeneity at capsid-premembrane (C-prM), envelope (E), nonstructural protein 3 (NS3), and NS5. The extentof sequence heterogeneity revealed by clonal sequencing was genomic region-dependent, whereas the NS3 and NS5 had lower sequence heterogeneity than C-prM and E. Interestingly, the Phylogenetic Analysis by Maximum Likelihood program (PAML) analysis supported that the domain III of E region, the most heterogeneous region analyzed, was under the influence of positive selection.</p> <p>Conclusion</p> <p>This study confirmed previous reports that the most heterogeneous region of the dengue viral genome resided at the envelope region, of which the domain III was under positive selection pressure. Further studies will need to address the influence of these mutations on the overall fitness in different hosts (i.e., mosquito and human) during dengue viral transmission.</p> |
| first_indexed | 2024-12-11T14:36:44Z |
| format | Article |
| id | doaj.art-e3f93ba81fc84126af6b2ecac3c796fe |
| institution | Directory Open Access Journal |
| issn | 1743-422X |
| language | English |
| last_indexed | 2024-12-11T14:36:44Z |
| publishDate | 2005-08-01 |
| publisher | BMC |
| record_format | Article |
| series | Virology Journal |
| spelling | doaj.art-e3f93ba81fc84126af6b2ecac3c796fe2022-12-22T01:02:09ZengBMCVirology Journal1743-422X2005-08-01217210.1186/1743-422X-2-72Strategically examining the full-genome of dengue virus type 3 in clinical isolates reveals its mutation spectraWu Hui-LinChen Wei-JuneWang Wei-KungKing Chwan-ChuenChao Day-YuChang Gwong-Jen J<p>Abstract</p> <p>Background</p> <p>Previous studies presented the quasispecies spectrum of the envelope region of dengue virus type 3 (DENV-3) from either clinical specimens or field-caught mosquitoes. However, the extent of sequence variation among full genomic sequences of DENV within infected individuals remains largely unknown.</p> <p>Results</p> <p>Instead of arbitrarily choosing one genomic region in this study, the full genomic consensus sequences of six DENV-3 isolates were used to locate four genomic regions that had a higher potential of sequence heterogeneity at capsid-premembrane (C-prM), envelope (E), nonstructural protein 3 (NS3), and NS5. The extentof sequence heterogeneity revealed by clonal sequencing was genomic region-dependent, whereas the NS3 and NS5 had lower sequence heterogeneity than C-prM and E. Interestingly, the Phylogenetic Analysis by Maximum Likelihood program (PAML) analysis supported that the domain III of E region, the most heterogeneous region analyzed, was under the influence of positive selection.</p> <p>Conclusion</p> <p>This study confirmed previous reports that the most heterogeneous region of the dengue viral genome resided at the envelope region, of which the domain III was under positive selection pressure. Further studies will need to address the influence of these mutations on the overall fitness in different hosts (i.e., mosquito and human) during dengue viral transmission.</p>http://www.virologyj.com/content/2/1/72Quasispeciesmutation spectramicro-evolution of dengue virus serotype 3dengue hemorrhagic fever (DHF)sequence diversityTaiwan |
| spellingShingle | Wu Hui-Lin Chen Wei-June Wang Wei-Kung King Chwan-Chuen Chao Day-Yu Chang Gwong-Jen J Strategically examining the full-genome of dengue virus type 3 in clinical isolates reveals its mutation spectra Virology Journal Quasispecies mutation spectra micro-evolution of dengue virus serotype 3 dengue hemorrhagic fever (DHF) sequence diversity Taiwan |
| title | Strategically examining the full-genome of dengue virus type 3 in clinical isolates reveals its mutation spectra |
| title_full | Strategically examining the full-genome of dengue virus type 3 in clinical isolates reveals its mutation spectra |
| title_fullStr | Strategically examining the full-genome of dengue virus type 3 in clinical isolates reveals its mutation spectra |
| title_full_unstemmed | Strategically examining the full-genome of dengue virus type 3 in clinical isolates reveals its mutation spectra |
| title_short | Strategically examining the full-genome of dengue virus type 3 in clinical isolates reveals its mutation spectra |
| title_sort | strategically examining the full genome of dengue virus type 3 in clinical isolates reveals its mutation spectra |
| topic | Quasispecies mutation spectra micro-evolution of dengue virus serotype 3 dengue hemorrhagic fever (DHF) sequence diversity Taiwan |
| url | http://www.virologyj.com/content/2/1/72 |
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