Role of Wet Mount and Cytospin Smears in Diagnosing Urothelial Carcinoma: A Descriptive Observational Study
Introduction: Most common carcinomas of lower urinary tract are of urothelial type in which, majority of them occur in the bladder. The demonstration of exfoliated neoplastic cells in urine sediment cytology is one of the choices of investigation. Aim: To study the morphology of atypical cells o...
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| Format: | Article |
| Language: | English |
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JCDR Research and Publications Pvt. Ltd.
2022-04-01
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| Series: | National Journal of Laboratory Medicine |
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| Online Access: | https://www.njlm.net/articles/PDF/2600/50727_CE[Ra1]_F(SHU)_PF1(TW_SHU)_PFA_NC(SHU)_PN(SHU).pdf |
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| author | Asha Patil P Shashikala Pratima M Pattar K Arpitha |
| author_facet | Asha Patil P Shashikala Pratima M Pattar K Arpitha |
| author_sort | Asha Patil |
| collection | DOAJ |
| description | Introduction: Most common carcinomas of lower urinary tract
are of urothelial type in which, majority of them occur in the
bladder. The demonstration of exfoliated neoplastic cells in
urine sediment cytology is one of the choices of investigation.
Aim: To study the morphology of atypical cells of urine sediment
in unstained wet mount preparation and urine sediment obtained
by cytospin smear stained with Papanicolaou stain.
Materials and Methods: It was a descriptive observational
study done over a period of 29 months i.e., from July 2016
to December 2018 at SS Institute of Medical Sciences and
Research Centre, Davangere, Karnataka, India. Using non
probability sampling method, 50 urine samples were analysed
by DIRUI-H 500 a semi automatic urine analyser, which were
further examined by wet mount preparation and cytospin smear
stained with papanicolaou stain. Results were analysed by
whether the smears were positive or negative for atypical cells
based on the cytological and nuclear details.
Results: The yield of Red Blood Corpuscles (RBCs) was
significantly increased in cytospin preparation with morphology
of RBCs was better appreciated in wet mount compared to
cytospin preparation. The yield of atypical cells was significantly
increased in cytospin preparation with the morphology of
atypical cells were better appreciated in pap stained cytospin
preparation compared to wet mount urine examination.
Conclusion: Overall, cell yield and preservation of White Blood
Corpuscles (WBCs), epithelial cell and atypical cells morphology
was better in cytospin preparation, while RBCs were better
appreciated in wet mount preparation. Cytospin technology is
a quick, efficient and cost effective method for increasing cell
yield in less cellular samples and also helps in providing better
cellular morphological details. |
| first_indexed | 2024-12-11T23:03:31Z |
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| id | doaj.art-e3fb24b6f5224b3fbd2dd749db21f37a |
| institution | Directory Open Access Journal |
| issn | 2277-8551 2455-6882 |
| language | English |
| last_indexed | 2024-12-11T23:03:31Z |
| publishDate | 2022-04-01 |
| publisher | JCDR Research and Publications Pvt. Ltd. |
| record_format | Article |
| series | National Journal of Laboratory Medicine |
| spelling | doaj.art-e3fb24b6f5224b3fbd2dd749db21f37a2022-12-22T00:47:01ZengJCDR Research and Publications Pvt. Ltd.National Journal of Laboratory Medicine2277-85512455-68822022-04-011102242710.7860/NJLM/2022/50727.2600Role of Wet Mount and Cytospin Smears in Diagnosing Urothelial Carcinoma: A Descriptive Observational StudyAsha Patil0P Shashikala1Pratima M Pattar2K Arpitha3Assistant Professor, Department of Pathology, ESIC Medical College and Hospital, Kalaburagi, Karnataka, India.Professor and Head, Department of Pathology, SS Institute of Medical Sciences and Research Centre, Davangere, Karnataka, India.Assistant Professor, Department of Pathology, ESIC Medical College and Hospital, Kalaburagi, Karnataka, India.Senior Resident, Department of Pathology, ESIC Medical College and Hospital, Kalaburagi, Karnataka, India.Introduction: Most common carcinomas of lower urinary tract are of urothelial type in which, majority of them occur in the bladder. The demonstration of exfoliated neoplastic cells in urine sediment cytology is one of the choices of investigation. Aim: To study the morphology of atypical cells of urine sediment in unstained wet mount preparation and urine sediment obtained by cytospin smear stained with Papanicolaou stain. Materials and Methods: It was a descriptive observational study done over a period of 29 months i.e., from July 2016 to December 2018 at SS Institute of Medical Sciences and Research Centre, Davangere, Karnataka, India. Using non probability sampling method, 50 urine samples were analysed by DIRUI-H 500 a semi automatic urine analyser, which were further examined by wet mount preparation and cytospin smear stained with papanicolaou stain. Results were analysed by whether the smears were positive or negative for atypical cells based on the cytological and nuclear details. Results: The yield of Red Blood Corpuscles (RBCs) was significantly increased in cytospin preparation with morphology of RBCs was better appreciated in wet mount compared to cytospin preparation. The yield of atypical cells was significantly increased in cytospin preparation with the morphology of atypical cells were better appreciated in pap stained cytospin preparation compared to wet mount urine examination. Conclusion: Overall, cell yield and preservation of White Blood Corpuscles (WBCs), epithelial cell and atypical cells morphology was better in cytospin preparation, while RBCs were better appreciated in wet mount preparation. Cytospin technology is a quick, efficient and cost effective method for increasing cell yield in less cellular samples and also helps in providing better cellular morphological details.https://www.njlm.net/articles/PDF/2600/50727_CE[Ra1]_F(SHU)_PF1(TW_SHU)_PFA_NC(SHU)_PN(SHU).pdfpapanicolaou stainurinary tracturine analysisurine sediment cytology |
| spellingShingle | Asha Patil P Shashikala Pratima M Pattar K Arpitha Role of Wet Mount and Cytospin Smears in Diagnosing Urothelial Carcinoma: A Descriptive Observational Study National Journal of Laboratory Medicine papanicolaou stain urinary tract urine analysis urine sediment cytology |
| title | Role of Wet Mount and Cytospin Smears in Diagnosing Urothelial Carcinoma: A Descriptive Observational Study |
| title_full | Role of Wet Mount and Cytospin Smears in Diagnosing Urothelial Carcinoma: A Descriptive Observational Study |
| title_fullStr | Role of Wet Mount and Cytospin Smears in Diagnosing Urothelial Carcinoma: A Descriptive Observational Study |
| title_full_unstemmed | Role of Wet Mount and Cytospin Smears in Diagnosing Urothelial Carcinoma: A Descriptive Observational Study |
| title_short | Role of Wet Mount and Cytospin Smears in Diagnosing Urothelial Carcinoma: A Descriptive Observational Study |
| title_sort | role of wet mount and cytospin smears in diagnosing urothelial carcinoma a descriptive observational study |
| topic | papanicolaou stain urinary tract urine analysis urine sediment cytology |
| url | https://www.njlm.net/articles/PDF/2600/50727_CE[Ra1]_F(SHU)_PF1(TW_SHU)_PFA_NC(SHU)_PN(SHU).pdf |
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