Comparative analysis of the Gene expression profile of Chemokine Receptors between Adipose-derived and Bone marrow-derived Mesenchymal Stem Cells
Introduction: Mesenchymal stem cells (MSCs) hold great promise in the field of regenerative medicine.Although originally isolated from bone marrow, MSCs have since been obtained from a variety of adult and neonatal tissues including the adipose tissue. Stemness and multipotential features of Mesench...
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| Format: | Article |
| Language: | English |
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Mashhad University of Medical Sciences
2014-05-01
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| Series: | International Journal of Pediatrics |
| Subjects: | |
| Online Access: | http://ijp.mums.ac.ir/pdf_2666_9a5dd2b2e43d79b6aea2222a2ea92f05.html |
| _version_ | 1798040902132826112 |
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| author | Hamid Reza Bidkhori Moien Farshchian Asieh Heirani-Tabasi Hojjat Naderi-Meshkin1 Mahtab Dastpak Naghmeh Ahmadian Kia Ahmad Reza Bahrami Maryam M. Matin |
| author_facet | Hamid Reza Bidkhori Moien Farshchian Asieh Heirani-Tabasi Hojjat Naderi-Meshkin1 Mahtab Dastpak Naghmeh Ahmadian Kia Ahmad Reza Bahrami Maryam M. Matin |
| author_sort | Hamid Reza Bidkhori |
| collection | DOAJ |
| description | Introduction:
Mesenchymal stem cells (MSCs) hold great promise in the field of regenerative medicine.Although originally isolated from bone marrow, MSCs have since been obtained from a variety of adult and neonatal tissues including the adipose tissue. Stemness and multipotential features of Mesenchymal Stem Cells (MSC) has been highlighted in many studies but there are many dark aspects in exclusive capabilities of MSC derived from different sources. Regarding adipose-derived MSCs, Ad-MSCs, and bone marrow- derived MSC, BM-MSCs has been introduced and applicable in clinics, we designed a small molecule induction based approach for evaluation of gene expression response between these cells.
Materials and Methods:
A panel of MSC specific genes encoding CXCR4, CXCR6, CX3CR1 and CCR1 has been comparatively analyzed between Ad-MSCs and BM-MSCs by Real-Time PCR, in response to hypoxia- mimicking agents CoCl2, DFX and VPA.
Results:
Our results showed CXCR4 expression dramatically increased following DFX and VPA treatment in Ad-MSC but decreased in BM-MSCs, over expression of CCR1 was detected only in BM-MSCs but CXCR6 and CX3CR1didn't show any detectable expression between these kinds of MSCs.
Conclusion:
We speculate that differential expression profile of CXCR4 and CCR1 related to different capabilities of BM-MSCs and Ad-MSCs and further investigations necessary to determine which gene networks play the key roles in MSCs derived from different sources and these findings translated from bench to bed in novel cell therapies.
Keywords: Adipose-derived MSCs, Bone marrow-derived MSCs, Chemokine Receptors, Gene expression. |
| first_indexed | 2024-04-11T22:14:04Z |
| format | Article |
| id | doaj.art-e3fb4f61a0ce4fab9ad32dbf42b2769f |
| institution | Directory Open Access Journal |
| issn | 2345-5047 2345-5055 |
| language | English |
| last_indexed | 2024-04-11T22:14:04Z |
| publishDate | 2014-05-01 |
| publisher | Mashhad University of Medical Sciences |
| record_format | Article |
| series | International Journal of Pediatrics |
| spelling | doaj.art-e3fb4f61a0ce4fab9ad32dbf42b2769f2022-12-22T04:00:29ZengMashhad University of Medical SciencesInternational Journal of Pediatrics2345-50472345-50552014-05-0122.317172666Comparative analysis of the Gene expression profile of Chemokine Receptors between Adipose-derived and Bone marrow-derived Mesenchymal Stem CellsHamid Reza Bidkhori0Moien Farshchian1Asieh Heirani-Tabasi2Hojjat Naderi-Meshkin13Mahtab Dastpak4Naghmeh Ahmadian Kia5Ahmad Reza Bahrami6Maryam M. Matin7Stem Cells and Regenerative Medicine Research Department, ACECR-Khorasan Razavi branch, Mashhad, Iran.Department of Biology, Ferdowsi University of Mashhad, Mashhad, Iran.Stem Cells and Regenerative Medicine Research Department, ACECR-Khorasan Razavi branch, Mashhad, Iran.Stem Cells and Regenerative Medicine Research Department, ACECR-Khorasan Razavi branch, Mashhad, Iran.Department of Biology, Ferdowsi University of Mashhad, Mashhad, Iran.Shahrood University of Medical Sciences, Shahrood, Iran.Department of Biology, Ferdowsi University of Mashhad, Mashhad, Iran.Department of Biology, Ferdowsi University of Mashhad, Mashhad, Iran.Introduction: Mesenchymal stem cells (MSCs) hold great promise in the field of regenerative medicine.Although originally isolated from bone marrow, MSCs have since been obtained from a variety of adult and neonatal tissues including the adipose tissue. Stemness and multipotential features of Mesenchymal Stem Cells (MSC) has been highlighted in many studies but there are many dark aspects in exclusive capabilities of MSC derived from different sources. Regarding adipose-derived MSCs, Ad-MSCs, and bone marrow- derived MSC, BM-MSCs has been introduced and applicable in clinics, we designed a small molecule induction based approach for evaluation of gene expression response between these cells. Materials and Methods: A panel of MSC specific genes encoding CXCR4, CXCR6, CX3CR1 and CCR1 has been comparatively analyzed between Ad-MSCs and BM-MSCs by Real-Time PCR, in response to hypoxia- mimicking agents CoCl2, DFX and VPA. Results: Our results showed CXCR4 expression dramatically increased following DFX and VPA treatment in Ad-MSC but decreased in BM-MSCs, over expression of CCR1 was detected only in BM-MSCs but CXCR6 and CX3CR1didn't show any detectable expression between these kinds of MSCs. Conclusion: We speculate that differential expression profile of CXCR4 and CCR1 related to different capabilities of BM-MSCs and Ad-MSCs and further investigations necessary to determine which gene networks play the key roles in MSCs derived from different sources and these findings translated from bench to bed in novel cell therapies. Keywords: Adipose-derived MSCs, Bone marrow-derived MSCs, Chemokine Receptors, Gene expression.http://ijp.mums.ac.ir/pdf_2666_9a5dd2b2e43d79b6aea2222a2ea92f05.htmlOral Presentation |
| spellingShingle | Hamid Reza Bidkhori Moien Farshchian Asieh Heirani-Tabasi Hojjat Naderi-Meshkin1 Mahtab Dastpak Naghmeh Ahmadian Kia Ahmad Reza Bahrami Maryam M. Matin Comparative analysis of the Gene expression profile of Chemokine Receptors between Adipose-derived and Bone marrow-derived Mesenchymal Stem Cells International Journal of Pediatrics Oral Presentation |
| title | Comparative analysis of the Gene expression profile of Chemokine Receptors between Adipose-derived and Bone marrow-derived Mesenchymal Stem Cells |
| title_full | Comparative analysis of the Gene expression profile of Chemokine Receptors between Adipose-derived and Bone marrow-derived Mesenchymal Stem Cells |
| title_fullStr | Comparative analysis of the Gene expression profile of Chemokine Receptors between Adipose-derived and Bone marrow-derived Mesenchymal Stem Cells |
| title_full_unstemmed | Comparative analysis of the Gene expression profile of Chemokine Receptors between Adipose-derived and Bone marrow-derived Mesenchymal Stem Cells |
| title_short | Comparative analysis of the Gene expression profile of Chemokine Receptors between Adipose-derived and Bone marrow-derived Mesenchymal Stem Cells |
| title_sort | comparative analysis of the gene expression profile of chemokine receptors between adipose derived and bone marrow derived mesenchymal stem cells |
| topic | Oral Presentation |
| url | http://ijp.mums.ac.ir/pdf_2666_9a5dd2b2e43d79b6aea2222a2ea92f05.html |
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