Cell-homing and immunomodulatory composite hydrogels for effective wound healing with neovascularization
Wound healing in cases of excessive inflammation poses a significant challenge due to compromised neovascularization. Here, we propose a multi-functional composite hydrogel engineered to overcome such conditions through recruitment and activation of macrophages with adapted degradation of the hydrog...
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
KeAi Communications Co., Ltd.
2024-06-01
|
| Series: | Bioactive Materials |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2452199X24000744 |
| _version_ | 1797272451557621760 |
|---|---|
| author | Hayeon Byun Yujin Han Eunhyung Kim Indong Jun Jinkyu Lee Hyewoo Jeong Seung Jae Huh Jinmyoung Joo Su Ryon Shin Heungsoo Shin |
| author_facet | Hayeon Byun Yujin Han Eunhyung Kim Indong Jun Jinkyu Lee Hyewoo Jeong Seung Jae Huh Jinmyoung Joo Su Ryon Shin Heungsoo Shin |
| author_sort | Hayeon Byun |
| collection | DOAJ |
| description | Wound healing in cases of excessive inflammation poses a significant challenge due to compromised neovascularization. Here, we propose a multi-functional composite hydrogel engineered to overcome such conditions through recruitment and activation of macrophages with adapted degradation of the hydrogel. The composite hydrogel (G-TSrP) is created by combining gelatin methacryloyl (GelMA) and nanoparticles (TSrP) composed of tannic acid (TA) and Sr2+. These nanoparticles are prepared using a one-step mineralization process assisted by metal-phenolic network formation. G-TSrP exhibits the ability to eliminate reactive oxygen species and direct polarization of macrophages toward M2 phenotype. It has been observed that the liberation of TA and Sr2+ from G-TSrP actively facilitate the recruitment and up-regulation of the expression of extracellular matrix remodeling genes of macrophages, and thereby, coordinate in vivo adapted degradation of the G-TSrP. Most significantly, G-TSrP accelerates angiogenesis despite the TA's inhibitory properties, which are counteracted by the released Sr2+. Moreover, G-TSrP enhances wound closure under inflammation and promotes normal tissue formation with strong vessel growth. Genetic analysis confirms macrophage-mediated wound healing by the composite hydrogel. Collectively, these findings pave the way for the development of biomaterials that promote wound healing by creating regenerative environment. |
| first_indexed | 2024-03-07T14:28:24Z |
| format | Article |
| id | doaj.art-e3fde7a009c64d6887a67646b31fd012 |
| institution | Directory Open Access Journal |
| issn | 2452-199X |
| language | English |
| last_indexed | 2024-03-07T14:28:24Z |
| publishDate | 2024-06-01 |
| publisher | KeAi Communications Co., Ltd. |
| record_format | Article |
| series | Bioactive Materials |
| spelling | doaj.art-e3fde7a009c64d6887a67646b31fd0122024-03-06T05:28:27ZengKeAi Communications Co., Ltd.Bioactive Materials2452-199X2024-06-0136185202Cell-homing and immunomodulatory composite hydrogels for effective wound healing with neovascularizationHayeon Byun0Yujin Han1Eunhyung Kim2Indong Jun3Jinkyu Lee4Hyewoo Jeong5Seung Jae Huh6Jinmyoung Joo7Su Ryon Shin8Heungsoo Shin9Department of Bioengineering, Hanyang University, 222 Wangsimni-ro, Seongdong-gu, Seoul 04763, Republic of Korea; Division of Engineering in Medicine, Department of Medicine, Harvard Medical School, Brigham and Women's Hospital, Cambridge, MA 02139, USADepartment of Bioengineering, Hanyang University, 222 Wangsimni-ro, Seongdong-gu, Seoul 04763, Republic of KoreaDepartment of Bioengineering, Hanyang University, 222 Wangsimni-ro, Seongdong-gu, Seoul 04763, Republic of KoreaEnvironmental Safety Group, Korea Institute of Science & Technology Europe (KIST-EUROPE), Saarbrücken 66123, GermanyDepartment of Bioengineering, Hanyang University, 222 Wangsimni-ro, Seongdong-gu, Seoul 04763, Republic of KoreaDepartment of Bioengineering, Hanyang University, 222 Wangsimni-ro, Seongdong-gu, Seoul 04763, Republic of KoreaDepartment of Bioengineering, Hanyang University, 222 Wangsimni-ro, Seongdong-gu, Seoul 04763, Republic of KoreaDepartment of Biomedical Engineering, Ulsan National Institute of Science and Technology (UNIST), Ulsan 44919, Republic of KoreaDivision of Engineering in Medicine, Department of Medicine, Harvard Medical School, Brigham and Women's Hospital, Cambridge, MA 02139, USADepartment of Bioengineering, Hanyang University, 222 Wangsimni-ro, Seongdong-gu, Seoul 04763, Republic of Korea; Institute of Nano Science and Technology, Hanyang University, 222 Wangsimni-ro, Seongdong-gu, Seoul 04763, Republic of Korea; Corresponding author. Department of Bioengineering, Hanyang University, 222 Wangsimni-ro, Seongdong-gu, Seoul 04763, Republic of Korea.Wound healing in cases of excessive inflammation poses a significant challenge due to compromised neovascularization. Here, we propose a multi-functional composite hydrogel engineered to overcome such conditions through recruitment and activation of macrophages with adapted degradation of the hydrogel. The composite hydrogel (G-TSrP) is created by combining gelatin methacryloyl (GelMA) and nanoparticles (TSrP) composed of tannic acid (TA) and Sr2+. These nanoparticles are prepared using a one-step mineralization process assisted by metal-phenolic network formation. G-TSrP exhibits the ability to eliminate reactive oxygen species and direct polarization of macrophages toward M2 phenotype. It has been observed that the liberation of TA and Sr2+ from G-TSrP actively facilitate the recruitment and up-regulation of the expression of extracellular matrix remodeling genes of macrophages, and thereby, coordinate in vivo adapted degradation of the G-TSrP. Most significantly, G-TSrP accelerates angiogenesis despite the TA's inhibitory properties, which are counteracted by the released Sr2+. Moreover, G-TSrP enhances wound closure under inflammation and promotes normal tissue formation with strong vessel growth. Genetic analysis confirms macrophage-mediated wound healing by the composite hydrogel. Collectively, these findings pave the way for the development of biomaterials that promote wound healing by creating regenerative environment.http://www.sciencedirect.com/science/article/pii/S2452199X24000744ImmunomodulationWound healingNeovascularizationMulti-functional nanoparticlesComposite hydrogels |
| spellingShingle | Hayeon Byun Yujin Han Eunhyung Kim Indong Jun Jinkyu Lee Hyewoo Jeong Seung Jae Huh Jinmyoung Joo Su Ryon Shin Heungsoo Shin Cell-homing and immunomodulatory composite hydrogels for effective wound healing with neovascularization Bioactive Materials Immunomodulation Wound healing Neovascularization Multi-functional nanoparticles Composite hydrogels |
| title | Cell-homing and immunomodulatory composite hydrogels for effective wound healing with neovascularization |
| title_full | Cell-homing and immunomodulatory composite hydrogels for effective wound healing with neovascularization |
| title_fullStr | Cell-homing and immunomodulatory composite hydrogels for effective wound healing with neovascularization |
| title_full_unstemmed | Cell-homing and immunomodulatory composite hydrogels for effective wound healing with neovascularization |
| title_short | Cell-homing and immunomodulatory composite hydrogels for effective wound healing with neovascularization |
| title_sort | cell homing and immunomodulatory composite hydrogels for effective wound healing with neovascularization |
| topic | Immunomodulation Wound healing Neovascularization Multi-functional nanoparticles Composite hydrogels |
| url | http://www.sciencedirect.com/science/article/pii/S2452199X24000744 |
| work_keys_str_mv | AT hayeonbyun cellhomingandimmunomodulatorycompositehydrogelsforeffectivewoundhealingwithneovascularization AT yujinhan cellhomingandimmunomodulatorycompositehydrogelsforeffectivewoundhealingwithneovascularization AT eunhyungkim cellhomingandimmunomodulatorycompositehydrogelsforeffectivewoundhealingwithneovascularization AT indongjun cellhomingandimmunomodulatorycompositehydrogelsforeffectivewoundhealingwithneovascularization AT jinkyulee cellhomingandimmunomodulatorycompositehydrogelsforeffectivewoundhealingwithneovascularization AT hyewoojeong cellhomingandimmunomodulatorycompositehydrogelsforeffectivewoundhealingwithneovascularization AT seungjaehuh cellhomingandimmunomodulatorycompositehydrogelsforeffectivewoundhealingwithneovascularization AT jinmyoungjoo cellhomingandimmunomodulatorycompositehydrogelsforeffectivewoundhealingwithneovascularization AT suryonshin cellhomingandimmunomodulatorycompositehydrogelsforeffectivewoundhealingwithneovascularization AT heungsooshin cellhomingandimmunomodulatorycompositehydrogelsforeffectivewoundhealingwithneovascularization |