Identification of Collimonas gene loci involved in the biosynthesis of a diffusible secondary metabolite with broad‐spectrum antifungal activity and plant‐protective properties
Summary In greenhouse and field trials, a bacterial mixture of Collimonas arenae Cal35 and Bacillus velezensis FZB42, but not Cal35 alone or FZB42 alone, was able to protect tomato plants from challenge with the soilborne fungal pathogen Fusarium oxysporum f.sp. lycopersici (Fol). To identify genes...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2021-07-01
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| Series: | Microbial Biotechnology |
| Online Access: | https://doi.org/10.1111/1751-7915.13716 |
| _version_ | 1818456248874434560 |
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| author | Fidele N. Akum Ravi Kumar Gary Lai Catherine H. Williams Hung K. Doan Johan H.J. Leveau |
| author_facet | Fidele N. Akum Ravi Kumar Gary Lai Catherine H. Williams Hung K. Doan Johan H.J. Leveau |
| author_sort | Fidele N. Akum |
| collection | DOAJ |
| description | Summary In greenhouse and field trials, a bacterial mixture of Collimonas arenae Cal35 and Bacillus velezensis FZB42, but not Cal35 alone or FZB42 alone, was able to protect tomato plants from challenge with the soilborne fungal pathogen Fusarium oxysporum f.sp. lycopersici (Fol). To identify genes and mechanisms underlying this property in Cal35, we screened a random transposon insertion library for loss of function and identified two mutants that were impaired completely or partially in their ability to halt the growth of a wide range of fungal species. In mutant 46A06, the transposon insertion was located in a biosynthetic gene cluster that was predicted to code for a hybrid polyketide synthase–non‐ribosomal peptide synthetase, while mutant 60C09 was impacted in a gene cluster for the synthesis and secretion of sugar repeat units. Our data are consistent with a model in which both gene clusters are necessary for the production of an antifungal compound we refer to as carenaemins. We also show that the ability to produce carenaemin contributed significantly to the observed synergy between Cal35 and FZB42 in protecting tomato plants from Fol. We discuss the potential for supplementing Bacillus‐based biocontrol products with Collimonas bacteria to boost efficacy of such products. |
| first_indexed | 2024-12-14T22:23:40Z |
| format | Article |
| id | doaj.art-e3ff10f29f964fa393b479f15001a4be |
| institution | Directory Open Access Journal |
| issn | 1751-7915 |
| language | English |
| last_indexed | 2024-12-14T22:23:40Z |
| publishDate | 2021-07-01 |
| publisher | Wiley |
| record_format | Article |
| series | Microbial Biotechnology |
| spelling | doaj.art-e3ff10f29f964fa393b479f15001a4be2022-12-21T22:45:26ZengWileyMicrobial Biotechnology1751-79152021-07-011441367138410.1111/1751-7915.13716Identification of Collimonas gene loci involved in the biosynthesis of a diffusible secondary metabolite with broad‐spectrum antifungal activity and plant‐protective propertiesFidele N. Akum0Ravi Kumar1Gary Lai2Catherine H. Williams3Hung K. Doan4Johan H.J. Leveau5Department of Plant Pathology University of California Davis Davis CA USANovozymes Inc 1445 Drew Avenue Davis CA USANovozymes Inc 1445 Drew Avenue Davis CA USADepartment of Plant Pathology University of California Davis Davis CA USADepartment of Plant Pathology University of California Davis Davis CA USADepartment of Plant Pathology University of California Davis Davis CA USASummary In greenhouse and field trials, a bacterial mixture of Collimonas arenae Cal35 and Bacillus velezensis FZB42, but not Cal35 alone or FZB42 alone, was able to protect tomato plants from challenge with the soilborne fungal pathogen Fusarium oxysporum f.sp. lycopersici (Fol). To identify genes and mechanisms underlying this property in Cal35, we screened a random transposon insertion library for loss of function and identified two mutants that were impaired completely or partially in their ability to halt the growth of a wide range of fungal species. In mutant 46A06, the transposon insertion was located in a biosynthetic gene cluster that was predicted to code for a hybrid polyketide synthase–non‐ribosomal peptide synthetase, while mutant 60C09 was impacted in a gene cluster for the synthesis and secretion of sugar repeat units. Our data are consistent with a model in which both gene clusters are necessary for the production of an antifungal compound we refer to as carenaemins. We also show that the ability to produce carenaemin contributed significantly to the observed synergy between Cal35 and FZB42 in protecting tomato plants from Fol. We discuss the potential for supplementing Bacillus‐based biocontrol products with Collimonas bacteria to boost efficacy of such products.https://doi.org/10.1111/1751-7915.13716 |
| spellingShingle | Fidele N. Akum Ravi Kumar Gary Lai Catherine H. Williams Hung K. Doan Johan H.J. Leveau Identification of Collimonas gene loci involved in the biosynthesis of a diffusible secondary metabolite with broad‐spectrum antifungal activity and plant‐protective properties Microbial Biotechnology |
| title | Identification of Collimonas gene loci involved in the biosynthesis of a diffusible secondary metabolite with broad‐spectrum antifungal activity and plant‐protective properties |
| title_full | Identification of Collimonas gene loci involved in the biosynthesis of a diffusible secondary metabolite with broad‐spectrum antifungal activity and plant‐protective properties |
| title_fullStr | Identification of Collimonas gene loci involved in the biosynthesis of a diffusible secondary metabolite with broad‐spectrum antifungal activity and plant‐protective properties |
| title_full_unstemmed | Identification of Collimonas gene loci involved in the biosynthesis of a diffusible secondary metabolite with broad‐spectrum antifungal activity and plant‐protective properties |
| title_short | Identification of Collimonas gene loci involved in the biosynthesis of a diffusible secondary metabolite with broad‐spectrum antifungal activity and plant‐protective properties |
| title_sort | identification of collimonas gene loci involved in the biosynthesis of a diffusible secondary metabolite with broad spectrum antifungal activity and plant protective properties |
| url | https://doi.org/10.1111/1751-7915.13716 |
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