Anal infections with concomitant <it>Chlamydia trachomatis </it>genotypes among men who have sex with men in Amsterdam, the Netherlands

<p>Abstract</p> <p>Background</p> <p>Lymphogranuloma venereum (LGV) proctitis is caused by <it>Chlamydia trachomatis </it>(Ct) genotype L and is endemic among men who have sex with men (MSM) in western society. Genotype L infections need to be distinguished...

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Main Authors: van der Loeff Maarten, Bruisten Sylvia M, Quint Wim GV, Bom Reinier JM, Quint Koen D, Morré Servaas A, de Vries Henry JC
Format: Article
Language:English
Published: BMC 2011-03-01
Series:BMC Infectious Diseases
Online Access:http://www.biomedcentral.com/1471-2334/11/63
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author van der Loeff Maarten
Bruisten Sylvia M
Quint Wim GV
Bom Reinier JM
Quint Koen D
Morré Servaas A
de Vries Henry JC
author_facet van der Loeff Maarten
Bruisten Sylvia M
Quint Wim GV
Bom Reinier JM
Quint Koen D
Morré Servaas A
de Vries Henry JC
author_sort van der Loeff Maarten
collection DOAJ
description <p>Abstract</p> <p>Background</p> <p>Lymphogranuloma venereum (LGV) proctitis is caused by <it>Chlamydia trachomatis </it>(Ct) genotype L and is endemic among men who have sex with men (MSM) in western society. Genotype L infections need to be distinguished from non-LGV (genotypes A-K) Ct infections since they require prolonged antibiotic treatment. For this purpose, an in-house developed <it>pmpH </it>based LGV polymerase chain reaction (PCR) test is used at the Amsterdam STI outpatient clinic. We investigated retrospectively the anal Ct genotype distribution, and the frequency of concomitant genotype infections in MSM infected with LGV and non-LGV Ct infections. To detect concomitant Ct genotype infections, the <it>pmpH </it>LGV PCR and genoTyping Reverse Hybridization Assay (Ct-DT RHA) were used.</p> <p>Methods</p> <p>A total of 201 Ct positive rectal swabs from MSM were selected, which were previously diagnosed as either LGV (n = 99) or non-LGV Ct infection (n = 102) according to the algorithm of Ct detection by the commercially available Aptima Combo 2 assay followed by an in-house <it>pmpH </it>LGV PCR. The samples were retested with the commercially available Ct-DT RHA, which differentiates between 14 major genotypes and is able to detect concomitant Ct genotypes.</p> <p>Results</p> <p>Excellent genotyping agreement was observed between the Ct-DT RHA and the <it>pmpH </it>LGV PCR (Kappa = 0.900, 95%CI = 0.845-0.955, McNemar's p = 1.000). A concomitant non-LGV genotype was detected in 6/99 (6.1%) LGV samples. No additional LGV infections were observed with the Ct-DT RHA among the non-LGV Ct group. In the non-LGV group genotype G/Ga (34.3%) was seen most frequent, followed by genotype D/Da (22.5%) and genotype J (13.7%). All LGV infections were caused by genotype L2.</p> <p>Conclusions</p> <p>Concomitant non-LGV genotypes do not lead to missed LGV proctitis diagnosis. The <it>pmpH </it>LGV PCR displayed excellent agreement with the commercially available Ct-DT genotyping RHA test. The genotypes G/Ga, D/Da and J were the most frequent non-LGV Ct strains in MSM.</p>
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spelling doaj.art-e404d534d5c74aa3afecd9855db042d02022-12-22T01:01:28ZengBMCBMC Infectious Diseases1471-23342011-03-011116310.1186/1471-2334-11-63Anal infections with concomitant <it>Chlamydia trachomatis </it>genotypes among men who have sex with men in Amsterdam, the Netherlandsvan der Loeff MaartenBruisten Sylvia MQuint Wim GVBom Reinier JMQuint Koen DMorré Servaas Ade Vries Henry JC<p>Abstract</p> <p>Background</p> <p>Lymphogranuloma venereum (LGV) proctitis is caused by <it>Chlamydia trachomatis </it>(Ct) genotype L and is endemic among men who have sex with men (MSM) in western society. Genotype L infections need to be distinguished from non-LGV (genotypes A-K) Ct infections since they require prolonged antibiotic treatment. For this purpose, an in-house developed <it>pmpH </it>based LGV polymerase chain reaction (PCR) test is used at the Amsterdam STI outpatient clinic. We investigated retrospectively the anal Ct genotype distribution, and the frequency of concomitant genotype infections in MSM infected with LGV and non-LGV Ct infections. To detect concomitant Ct genotype infections, the <it>pmpH </it>LGV PCR and genoTyping Reverse Hybridization Assay (Ct-DT RHA) were used.</p> <p>Methods</p> <p>A total of 201 Ct positive rectal swabs from MSM were selected, which were previously diagnosed as either LGV (n = 99) or non-LGV Ct infection (n = 102) according to the algorithm of Ct detection by the commercially available Aptima Combo 2 assay followed by an in-house <it>pmpH </it>LGV PCR. The samples were retested with the commercially available Ct-DT RHA, which differentiates between 14 major genotypes and is able to detect concomitant Ct genotypes.</p> <p>Results</p> <p>Excellent genotyping agreement was observed between the Ct-DT RHA and the <it>pmpH </it>LGV PCR (Kappa = 0.900, 95%CI = 0.845-0.955, McNemar's p = 1.000). A concomitant non-LGV genotype was detected in 6/99 (6.1%) LGV samples. No additional LGV infections were observed with the Ct-DT RHA among the non-LGV Ct group. In the non-LGV group genotype G/Ga (34.3%) was seen most frequent, followed by genotype D/Da (22.5%) and genotype J (13.7%). All LGV infections were caused by genotype L2.</p> <p>Conclusions</p> <p>Concomitant non-LGV genotypes do not lead to missed LGV proctitis diagnosis. The <it>pmpH </it>LGV PCR displayed excellent agreement with the commercially available Ct-DT genotyping RHA test. The genotypes G/Ga, D/Da and J were the most frequent non-LGV Ct strains in MSM.</p>http://www.biomedcentral.com/1471-2334/11/63
spellingShingle van der Loeff Maarten
Bruisten Sylvia M
Quint Wim GV
Bom Reinier JM
Quint Koen D
Morré Servaas A
de Vries Henry JC
Anal infections with concomitant <it>Chlamydia trachomatis </it>genotypes among men who have sex with men in Amsterdam, the Netherlands
BMC Infectious Diseases
title Anal infections with concomitant <it>Chlamydia trachomatis </it>genotypes among men who have sex with men in Amsterdam, the Netherlands
title_full Anal infections with concomitant <it>Chlamydia trachomatis </it>genotypes among men who have sex with men in Amsterdam, the Netherlands
title_fullStr Anal infections with concomitant <it>Chlamydia trachomatis </it>genotypes among men who have sex with men in Amsterdam, the Netherlands
title_full_unstemmed Anal infections with concomitant <it>Chlamydia trachomatis </it>genotypes among men who have sex with men in Amsterdam, the Netherlands
title_short Anal infections with concomitant <it>Chlamydia trachomatis </it>genotypes among men who have sex with men in Amsterdam, the Netherlands
title_sort anal infections with concomitant it chlamydia trachomatis it genotypes among men who have sex with men in amsterdam the netherlands
url http://www.biomedcentral.com/1471-2334/11/63
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