Effect of the addition of cellulolytic bacteria to ruminal bacteria on in vitro fermentation characteristics
Background: Digestibility of fiber in the rumen is not due to enzymatic activity of individual bacteria, but rather to their interaction, which complements their enzymatic functioning. Thus, efficiency of fiber digestion depends on the diversity and density of cellulolytic bacteria. Objective: To es...
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| Format: | Article |
| Language: | English |
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Universidad de Antioquia
2022-04-01
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| Series: | Revista Colombiana de Ciencias Pecuarias |
| Subjects: | |
| Online Access: | https://revistas.udea.edu.co/index.php/rccp/article/view/349418 |
| _version_ | 1797384775880671232 |
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| author | Nicolás Torres-Salado Marco-Antonio Ayala-Monter Paulino Sánchez-Santillán Isaac Almaraz-Buendía |
| author_facet | Nicolás Torres-Salado Marco-Antonio Ayala-Monter Paulino Sánchez-Santillán Isaac Almaraz-Buendía |
| author_sort | Nicolás Torres-Salado |
| collection | DOAJ |
| description | Background: Digestibility of fiber in the rumen is not due to enzymatic activity of individual bacteria, but rather to their interaction, which complements their enzymatic functioning. Thus, efficiency of fiber digestion depends on the diversity and density of cellulolytic bacteria. Objective: To estimate in vitro production of biogas, methane, and fermentative characteristics of cobra grass (Brachiaria hibrido) inoculated with ruminal bacteria (RB) in coculture with isolated cellulolytic bacteria (ICB) from bovine (ICBbov) or water buffalo (ICBbuf). Methods: ICBbov and ICBbuf were isolated from ruminal cellulolytic bacteria consortia using specific culture media for cellulolytic bacteria. Both were morphologically characterized and a Gram stain was performed. In the in vitro gas production test, the substrate was cobra grass and the inocula were ruminal bacteria (RB), ICBbov, ICBbuf, Coculturebov (RB + ICBbov) and Coculturebuf (RB + ICBbuf). Biogas and methane (CH4) production, as well as dry matter degradation (DMD) and neutral detergent fiber degradation (NDFD) were measured. A completely randomized design was used. Results: The ICB obtained were Gram positive cocci. Accumulated biogas production at 72 h from ICBbov and ICBbuf was on average 42.11% of that produced by RB. The Coculturebov produced 14.24% more biogas than RB. The CH4 production was lower in ICBbov and ICBbuf than in RB, Coculturebov and Coculturebuf. The DMD and NDFD were not different among RB, Coculturebov and Coculturebuf. The ICBbov degraded 37.10 and 96.34% more DMD and NDFD than ICBbuf (p<0.05). Conclusion: The use of ICB from bovine or water buffalo in coculture with RB does not improve in vitro production of biogas, DMD or NDFD with respect to RB alone. |
| first_indexed | 2024-03-08T21:40:33Z |
| format | Article |
| id | doaj.art-e405579ee5264f73b534d96761a95c42 |
| institution | Directory Open Access Journal |
| issn | 2256-2958 |
| language | English |
| last_indexed | 2024-03-08T21:40:33Z |
| publishDate | 2022-04-01 |
| publisher | Universidad de Antioquia |
| record_format | Article |
| series | Revista Colombiana de Ciencias Pecuarias |
| spelling | doaj.art-e405579ee5264f73b534d96761a95c422023-12-20T13:51:52ZengUniversidad de AntioquiaRevista Colombiana de Ciencias Pecuarias2256-29582022-04-01361223210.17533/udea.rccp.v35n4a567167Effect of the addition of cellulolytic bacteria to ruminal bacteria on in vitro fermentation characteristicsNicolás Torres-Salado0Marco-Antonio Ayala-Monter1Paulino Sánchez-SantillánIsaac Almaraz-Buendía2Universidad Autónoma de GuerreroUniversidad Autónoma de GuerreroUniversidad Autónoma del Estado de HidalgoBackground: Digestibility of fiber in the rumen is not due to enzymatic activity of individual bacteria, but rather to their interaction, which complements their enzymatic functioning. Thus, efficiency of fiber digestion depends on the diversity and density of cellulolytic bacteria. Objective: To estimate in vitro production of biogas, methane, and fermentative characteristics of cobra grass (Brachiaria hibrido) inoculated with ruminal bacteria (RB) in coculture with isolated cellulolytic bacteria (ICB) from bovine (ICBbov) or water buffalo (ICBbuf). Methods: ICBbov and ICBbuf were isolated from ruminal cellulolytic bacteria consortia using specific culture media for cellulolytic bacteria. Both were morphologically characterized and a Gram stain was performed. In the in vitro gas production test, the substrate was cobra grass and the inocula were ruminal bacteria (RB), ICBbov, ICBbuf, Coculturebov (RB + ICBbov) and Coculturebuf (RB + ICBbuf). Biogas and methane (CH4) production, as well as dry matter degradation (DMD) and neutral detergent fiber degradation (NDFD) were measured. A completely randomized design was used. Results: The ICB obtained were Gram positive cocci. Accumulated biogas production at 72 h from ICBbov and ICBbuf was on average 42.11% of that produced by RB. The Coculturebov produced 14.24% more biogas than RB. The CH4 production was lower in ICBbov and ICBbuf than in RB, Coculturebov and Coculturebuf. The DMD and NDFD were not different among RB, Coculturebov and Coculturebuf. The ICBbov degraded 37.10 and 96.34% more DMD and NDFD than ICBbuf (p<0.05). Conclusion: The use of ICB from bovine or water buffalo in coculture with RB does not improve in vitro production of biogas, DMD or NDFD with respect to RB alone.https://revistas.udea.edu.co/index.php/rccp/article/view/349418bacteriabiogasbovinebuffalocellulolitic bacteriacoculturefiber degradationfiberfermentation characteristicsgas productionin vitro fermentationmethanerumenruminal bacteria |
| spellingShingle | Nicolás Torres-Salado Marco-Antonio Ayala-Monter Paulino Sánchez-Santillán Isaac Almaraz-Buendía Effect of the addition of cellulolytic bacteria to ruminal bacteria on in vitro fermentation characteristics Revista Colombiana de Ciencias Pecuarias bacteria biogas bovine buffalo cellulolitic bacteria coculture fiber degradation fiber fermentation characteristics gas production in vitro fermentation methane rumen ruminal bacteria |
| title | Effect of the addition of cellulolytic bacteria to ruminal bacteria on in vitro fermentation characteristics |
| title_full | Effect of the addition of cellulolytic bacteria to ruminal bacteria on in vitro fermentation characteristics |
| title_fullStr | Effect of the addition of cellulolytic bacteria to ruminal bacteria on in vitro fermentation characteristics |
| title_full_unstemmed | Effect of the addition of cellulolytic bacteria to ruminal bacteria on in vitro fermentation characteristics |
| title_short | Effect of the addition of cellulolytic bacteria to ruminal bacteria on in vitro fermentation characteristics |
| title_sort | effect of the addition of cellulolytic bacteria to ruminal bacteria on in vitro fermentation characteristics |
| topic | bacteria biogas bovine buffalo cellulolitic bacteria coculture fiber degradation fiber fermentation characteristics gas production in vitro fermentation methane rumen ruminal bacteria |
| url | https://revistas.udea.edu.co/index.php/rccp/article/view/349418 |
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