N20D/N116E Combined Mutant Downward Shifted the pH Optimum of <i>Bacillus subtilis</i> NADH Oxidase

N20D/N116E Combined Mutant Downward Shifted the pH Optimum of <i>Bacillus subtilis</i> NADH Oxidase

Cofactor regeneration is indispensable to avoid the addition of large quantities of cofactor NADH or NAD<sup>+</sup> in oxidation-reduction reactions. Water-forming NADH oxidase (Nox) has attracted substantive attention as it can oxidize cytosolic NADH to NAD<sup>+</sup> with...

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Bibliographic Details
Main Authors:	Taowei Yang, Longze Pan, Wenhui Wu, Xuewei Pan, Meijuan Xu, Xian Zhang, Zhiming Rao
Format:	Article
Language:	English
Published:	MDPI AG 2023-03-01
Series:	Biology
Subjects:	water-forming NADH oxidase pH shifting NAD<sup>+</sup> regeneration
Online Access:	https://www.mdpi.com/2079-7737/12/4/522

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