Biotechnological Production of Poly(3-Hydroxybutyrate-<i>co</i>-4-Hydroxybutyrate-<i>co</i>-3-Hydroxyvalerate) Terpolymer by <i>Cupriavidus</i> sp. DSM 19379
The terpolymer of 3-hydroxybutyrate (3HB), 3-hydroxyvalerate (3HV), and 4-hydroxybutyrate (4HB) was produced employing <i>Cupriavidus</i> sp. DSM 19379. Growth in the presence of γ-butyrolactone, ε-caprolactone, 1,4-butanediol, and 1,6-hexanediol resulted in the synthes...
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MDPI AG
2019-08-01
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| Series: | Bioengineering |
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| Online Access: | https://www.mdpi.com/2306-5354/6/3/74 |
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| author | Dan Kucera Ivana Novackova Iva Pernicova Petr Sedlacek Stanislav Obruca |
| author_facet | Dan Kucera Ivana Novackova Iva Pernicova Petr Sedlacek Stanislav Obruca |
| author_sort | Dan Kucera |
| collection | DOAJ |
| description | The terpolymer of 3-hydroxybutyrate (3HB), 3-hydroxyvalerate (3HV), and 4-hydroxybutyrate (4HB) was produced employing <i>Cupriavidus</i> sp. DSM 19379. Growth in the presence of γ-butyrolactone, ε-caprolactone, 1,4-butanediol, and 1,6-hexanediol resulted in the synthesis of a polymer consisting of 3HB and 4HB monomers. Single and two-stage terpolymer production strategies were utilized to incorporate the 3HV subunit into the polymer structure. At the single-stage cultivation mode, γ-butyrolactone or 1,4-butanediol served as the primary substrate and propionic and valeric acid as the precursor of 3HV. In the two-stage production, glycerol was used in the growth phase, and precursors for the formation of the terpolymer in combination with the nitrogen limitation in the medium were used in the second phase. The aim of this work was to maximize the Polyhydroxyalkanoates (PHA) yields with a high proportion of 3HV and 4HB using different culture strategies. The obtained polymers contained 0−29 mol% of 3HV and 16−32 mol% of 4HB. Selected polymers were subjected to a material properties analysis such as differential scanning calorimetry (DSC), thermogravimetry, and size exclusion chromatography coupled with multi angle light scattering (SEC-MALS) for determination of the molecular weight. The number of polymers in the biomass, as well as the monomer composition of the polymer were determined by gas chromatography. |
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| last_indexed | 2024-03-12T05:37:00Z |
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| spelling | doaj.art-e406dce1086c49a4b408720dc59edf222023-09-03T06:23:53ZengMDPI AGBioengineering2306-53542019-08-01637410.3390/bioengineering6030074bioengineering6030074Biotechnological Production of Poly(3-Hydroxybutyrate-<i>co</i>-4-Hydroxybutyrate-<i>co</i>-3-Hydroxyvalerate) Terpolymer by <i>Cupriavidus</i> sp. DSM 19379Dan Kucera0Ivana Novackova1Iva Pernicova2Petr Sedlacek3Stanislav Obruca4Faculty of Chemistry, Brno University of Technology, Purkynova 118, 612 00 Brno, Czech RepublicFaculty of Chemistry, Brno University of Technology, Purkynova 118, 612 00 Brno, Czech RepublicFaculty of Chemistry, Brno University of Technology, Purkynova 118, 612 00 Brno, Czech RepublicMaterial Research Centre, Faculty of Chemistry, Brno University of Technology, Purkynova 118, 612 00 Brno, Czech RepublicFaculty of Chemistry, Brno University of Technology, Purkynova 118, 612 00 Brno, Czech RepublicThe terpolymer of 3-hydroxybutyrate (3HB), 3-hydroxyvalerate (3HV), and 4-hydroxybutyrate (4HB) was produced employing <i>Cupriavidus</i> sp. DSM 19379. Growth in the presence of γ-butyrolactone, ε-caprolactone, 1,4-butanediol, and 1,6-hexanediol resulted in the synthesis of a polymer consisting of 3HB and 4HB monomers. Single and two-stage terpolymer production strategies were utilized to incorporate the 3HV subunit into the polymer structure. At the single-stage cultivation mode, γ-butyrolactone or 1,4-butanediol served as the primary substrate and propionic and valeric acid as the precursor of 3HV. In the two-stage production, glycerol was used in the growth phase, and precursors for the formation of the terpolymer in combination with the nitrogen limitation in the medium were used in the second phase. The aim of this work was to maximize the Polyhydroxyalkanoates (PHA) yields with a high proportion of 3HV and 4HB using different culture strategies. The obtained polymers contained 0−29 mol% of 3HV and 16−32 mol% of 4HB. Selected polymers were subjected to a material properties analysis such as differential scanning calorimetry (DSC), thermogravimetry, and size exclusion chromatography coupled with multi angle light scattering (SEC-MALS) for determination of the molecular weight. The number of polymers in the biomass, as well as the monomer composition of the polymer were determined by gas chromatography.https://www.mdpi.com/2306-5354/6/3/74polyhydroxyalkanoatesterpolymerP(3HB-<i>co</i>-3HV-<i>co</i>-4HB)<i>Cupriavidus malaysiensis</i> |
| spellingShingle | Dan Kucera Ivana Novackova Iva Pernicova Petr Sedlacek Stanislav Obruca Biotechnological Production of Poly(3-Hydroxybutyrate-<i>co</i>-4-Hydroxybutyrate-<i>co</i>-3-Hydroxyvalerate) Terpolymer by <i>Cupriavidus</i> sp. DSM 19379 Bioengineering polyhydroxyalkanoates terpolymer P(3HB-<i>co</i>-3HV-<i>co</i>-4HB) <i>Cupriavidus malaysiensis</i> |
| title | Biotechnological Production of Poly(3-Hydroxybutyrate-<i>co</i>-4-Hydroxybutyrate-<i>co</i>-3-Hydroxyvalerate) Terpolymer by <i>Cupriavidus</i> sp. DSM 19379 |
| title_full | Biotechnological Production of Poly(3-Hydroxybutyrate-<i>co</i>-4-Hydroxybutyrate-<i>co</i>-3-Hydroxyvalerate) Terpolymer by <i>Cupriavidus</i> sp. DSM 19379 |
| title_fullStr | Biotechnological Production of Poly(3-Hydroxybutyrate-<i>co</i>-4-Hydroxybutyrate-<i>co</i>-3-Hydroxyvalerate) Terpolymer by <i>Cupriavidus</i> sp. DSM 19379 |
| title_full_unstemmed | Biotechnological Production of Poly(3-Hydroxybutyrate-<i>co</i>-4-Hydroxybutyrate-<i>co</i>-3-Hydroxyvalerate) Terpolymer by <i>Cupriavidus</i> sp. DSM 19379 |
| title_short | Biotechnological Production of Poly(3-Hydroxybutyrate-<i>co</i>-4-Hydroxybutyrate-<i>co</i>-3-Hydroxyvalerate) Terpolymer by <i>Cupriavidus</i> sp. DSM 19379 |
| title_sort | biotechnological production of poly 3 hydroxybutyrate i co i 4 hydroxybutyrate i co i 3 hydroxyvalerate terpolymer by i cupriavidus i sp dsm 19379 |
| topic | polyhydroxyalkanoates terpolymer P(3HB-<i>co</i>-3HV-<i>co</i>-4HB) <i>Cupriavidus malaysiensis</i> |
| url | https://www.mdpi.com/2306-5354/6/3/74 |
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