Mapping of Avirulence Genes in the Rice Blast Fungus, Magnaporthe grisea, with RFLP and RAPD Markers
Three genetically independent avirulence genes, AVR1-Irat7, AVR1-MedNoï, and AVR1-Ku86, were identified in a cross involving isolates Guy11 and 2/0/3 of the rice blast fungus, Magnaporthe grisea. Using 76 random progeny, we constructed a partial genetic map with restriction fragment length polymorph...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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The American Phytopathological Society
2000-02-01
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| Series: | Molecular Plant-Microbe Interactions |
| Online Access: | https://apsjournals.apsnet.org/doi/10.1094/MPMI.2000.13.2.217 |
| _version_ | 1818647048212185088 |
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| author | Waly Dioh Didier Tharreau Jean Loup Notteghem Marc Orbach Marc-Henri Lebrun |
| author_facet | Waly Dioh Didier Tharreau Jean Loup Notteghem Marc Orbach Marc-Henri Lebrun |
| author_sort | Waly Dioh |
| collection | DOAJ |
| description | Three genetically independent avirulence genes, AVR1-Irat7, AVR1-MedNoï, and AVR1-Ku86, were identified in a cross involving isolates Guy11 and 2/0/3 of the rice blast fungus, Magnaporthe grisea. Using 76 random progeny, we constructed a partial genetic map with restriction fragment length polymorphism (RFLP) markers revealed by probes such as the repeated sequences MGL/MGR583 and Pot3/MGR586, cosmids from the M. grisea genetic map, and a telomere sequence oligonucleotide. Avirulence genes AVR1-MedNoï and AVR1-Ku86 were closely linked to te-lomere RFLPs such as marker TelG (6 cM from AVR1-MedNoï) and TelF (4.5 cM from AVR1-Ku86). Avirulence gene AVR1-Irat7 was linked to a cosmid RFLP located on chromosome 1 and mapped at 20 cM from the avirulence gene AVR1-CO39. Using bulked segregant analysis, we identified 11 random amplified polymorphic DNA (RAPD) markers closely linked (0 to 10 cM) to the avirulence genes segregating in this cross. Most of these RAPD markers corresponded to junction fragments between known or new transposons and a single-copy sequence. Such junctions or the whole sequences of single-copy RAPD markers were frequently absent in one parental isolate. Single-copy sequences from RAPD markers tightly linked to avirulence genes will be used for positional cloning. |
| first_indexed | 2024-12-17T00:56:20Z |
| format | Article |
| id | doaj.art-e407c857b27b4bd4934cf2262efc92c5 |
| institution | Directory Open Access Journal |
| issn | 0894-0282 1943-7706 |
| language | English |
| last_indexed | 2024-12-17T00:56:20Z |
| publishDate | 2000-02-01 |
| publisher | The American Phytopathological Society |
| record_format | Article |
| series | Molecular Plant-Microbe Interactions |
| spelling | doaj.art-e407c857b27b4bd4934cf2262efc92c52022-12-21T22:09:36ZengThe American Phytopathological SocietyMolecular Plant-Microbe Interactions0894-02821943-77062000-02-0113221722710.1094/MPMI.2000.13.2.217Mapping of Avirulence Genes in the Rice Blast Fungus, Magnaporthe grisea, with RFLP and RAPD MarkersWaly DiohDidier TharreauJean Loup NotteghemMarc OrbachMarc-Henri LebrunThree genetically independent avirulence genes, AVR1-Irat7, AVR1-MedNoï, and AVR1-Ku86, were identified in a cross involving isolates Guy11 and 2/0/3 of the rice blast fungus, Magnaporthe grisea. Using 76 random progeny, we constructed a partial genetic map with restriction fragment length polymorphism (RFLP) markers revealed by probes such as the repeated sequences MGL/MGR583 and Pot3/MGR586, cosmids from the M. grisea genetic map, and a telomere sequence oligonucleotide. Avirulence genes AVR1-MedNoï and AVR1-Ku86 were closely linked to te-lomere RFLPs such as marker TelG (6 cM from AVR1-MedNoï) and TelF (4.5 cM from AVR1-Ku86). Avirulence gene AVR1-Irat7 was linked to a cosmid RFLP located on chromosome 1 and mapped at 20 cM from the avirulence gene AVR1-CO39. Using bulked segregant analysis, we identified 11 random amplified polymorphic DNA (RAPD) markers closely linked (0 to 10 cM) to the avirulence genes segregating in this cross. Most of these RAPD markers corresponded to junction fragments between known or new transposons and a single-copy sequence. Such junctions or the whole sequences of single-copy RAPD markers were frequently absent in one parental isolate. Single-copy sequences from RAPD markers tightly linked to avirulence genes will be used for positional cloning.https://apsjournals.apsnet.org/doi/10.1094/MPMI.2000.13.2.217 |
| spellingShingle | Waly Dioh Didier Tharreau Jean Loup Notteghem Marc Orbach Marc-Henri Lebrun Mapping of Avirulence Genes in the Rice Blast Fungus, Magnaporthe grisea, with RFLP and RAPD Markers Molecular Plant-Microbe Interactions |
| title | Mapping of Avirulence Genes in the Rice Blast Fungus, Magnaporthe grisea, with RFLP and RAPD Markers |
| title_full | Mapping of Avirulence Genes in the Rice Blast Fungus, Magnaporthe grisea, with RFLP and RAPD Markers |
| title_fullStr | Mapping of Avirulence Genes in the Rice Blast Fungus, Magnaporthe grisea, with RFLP and RAPD Markers |
| title_full_unstemmed | Mapping of Avirulence Genes in the Rice Blast Fungus, Magnaporthe grisea, with RFLP and RAPD Markers |
| title_short | Mapping of Avirulence Genes in the Rice Blast Fungus, Magnaporthe grisea, with RFLP and RAPD Markers |
| title_sort | mapping of avirulence genes in the rice blast fungus magnaporthe grisea with rflp and rapd markers |
| url | https://apsjournals.apsnet.org/doi/10.1094/MPMI.2000.13.2.217 |
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