Assessment of oxidative stress response genes in Avicennia marina exposed to oil contamination – Polyphenol oxidase (PPOA) as a biomarker
Mangrove plants, which inhabit and form sensitive ecosystems in the intertidal zones of tropical and subtropical coastlines, though vulnerable to petroleum pollution, still maintain their growth under oil contamination. To elucidate the molecular response of mangrove plants to crude oil–sediment mix...
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| Format: | Article |
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Elsevier
2020-12-01
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| Series: | Biotechnology Reports |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2215017X20305725 |
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| author | Babak Moradi Ralph Kissen Hassan Zare Maivan Mehri Seyed Hashtroudi Mona Sorahinobar Torfinn Sparstad Atle M. Bones |
| author_facet | Babak Moradi Ralph Kissen Hassan Zare Maivan Mehri Seyed Hashtroudi Mona Sorahinobar Torfinn Sparstad Atle M. Bones |
| author_sort | Babak Moradi |
| collection | DOAJ |
| description | Mangrove plants, which inhabit and form sensitive ecosystems in the intertidal zones of tropical and subtropical coastlines, though vulnerable to petroleum pollution, still maintain their growth under oil contamination. To elucidate the molecular response of mangrove plants to crude oil–sediment mixture, seeds of Avicennia marina were planted and grown on 0, 2.5, 5.0, 7.5 and10 % (w/w) oil-contaminated soil. Plant biomass was highly affected from 3.05 ± 0.28 (Control) to 0.50 ± .07 (10 %) and from 3.47 ± 0.12 to 1.88 ± 0.08 in 2 and 4 months old plants respectively. The expression analysis of 11genes belonging to detoxification pathways in the roots and leaves of 2 and 4 month-old plants was evaluated by qRT-PCR. Our results showed changes in expression levels of Fe-SOD, Mn-SOD, CAT, PRX, PPOs, GSTs, and NAP2 whose products are involved in reactive oxygen species (ROS) and xenobiotic detoxification. PPOA showed the highest expression induction of 43 ± 1.15, followed by CAT (12.61 ± 3.25) and PPOB (6.38 ± 1.34) in leaves of 2 months old seedlings grown on 7.5, 10 and 7.5 % oil contaminated soil respectively. PPOA (39.23 ± 2.1), PRX (32.13 ± 1.2) as well as PPOB (26.11 ± 1.3) showed the highest expression induction in leaves of 4 months old plants grown in 2.5 % oil contaminated soil. Our data indicated that PPOA can be a good biomarker candidate gene for long term exposure to oil contamination in A. marina. |
| first_indexed | 2024-12-20T02:28:26Z |
| format | Article |
| id | doaj.art-e40c44004768453ca13ed61b6913e6d3 |
| institution | Directory Open Access Journal |
| issn | 2215-017X |
| language | English |
| last_indexed | 2024-12-20T02:28:26Z |
| publishDate | 2020-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Biotechnology Reports |
| spelling | doaj.art-e40c44004768453ca13ed61b6913e6d32022-12-21T19:56:39ZengElsevierBiotechnology Reports2215-017X2020-12-0128e00565Assessment of oxidative stress response genes in Avicennia marina exposed to oil contamination – Polyphenol oxidase (PPOA) as a biomarkerBabak Moradi0Ralph Kissen1Hassan Zare Maivan2Mehri Seyed Hashtroudi3Mona Sorahinobar4Torfinn Sparstad5Atle M. Bones6Department of Plant Biology, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, IranCell Molecular Biology and Genomics Group, Department of Biology, Norwegian University of Science and Technology (NTNU), Trondheim, NorwayDepartment of Plant Biology, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran; Corresponding authors.Iranian National Institute for Oceanography and Atmospheric Science, Tehran, IranDepartment of Plant Biology, Faculty of Biological Science, Alzahra University, Tehran, IranCell Molecular Biology and Genomics Group, Department of Biology, Norwegian University of Science and Technology (NTNU), Trondheim, NorwayCell Molecular Biology and Genomics Group, Department of Biology, Norwegian University of Science and Technology (NTNU), Trondheim, Norway; Corresponding authors.Mangrove plants, which inhabit and form sensitive ecosystems in the intertidal zones of tropical and subtropical coastlines, though vulnerable to petroleum pollution, still maintain their growth under oil contamination. To elucidate the molecular response of mangrove plants to crude oil–sediment mixture, seeds of Avicennia marina were planted and grown on 0, 2.5, 5.0, 7.5 and10 % (w/w) oil-contaminated soil. Plant biomass was highly affected from 3.05 ± 0.28 (Control) to 0.50 ± .07 (10 %) and from 3.47 ± 0.12 to 1.88 ± 0.08 in 2 and 4 months old plants respectively. The expression analysis of 11genes belonging to detoxification pathways in the roots and leaves of 2 and 4 month-old plants was evaluated by qRT-PCR. Our results showed changes in expression levels of Fe-SOD, Mn-SOD, CAT, PRX, PPOs, GSTs, and NAP2 whose products are involved in reactive oxygen species (ROS) and xenobiotic detoxification. PPOA showed the highest expression induction of 43 ± 1.15, followed by CAT (12.61 ± 3.25) and PPOB (6.38 ± 1.34) in leaves of 2 months old seedlings grown on 7.5, 10 and 7.5 % oil contaminated soil respectively. PPOA (39.23 ± 2.1), PRX (32.13 ± 1.2) as well as PPOB (26.11 ± 1.3) showed the highest expression induction in leaves of 4 months old plants grown in 2.5 % oil contaminated soil. Our data indicated that PPOA can be a good biomarker candidate gene for long term exposure to oil contamination in A. marina.http://www.sciencedirect.com/science/article/pii/S2215017X20305725MangrovesOil pollutionGene expressionDetoxificationPolycyclic aromatic hydrocarbons (PAH)Biomarker |
| spellingShingle | Babak Moradi Ralph Kissen Hassan Zare Maivan Mehri Seyed Hashtroudi Mona Sorahinobar Torfinn Sparstad Atle M. Bones Assessment of oxidative stress response genes in Avicennia marina exposed to oil contamination – Polyphenol oxidase (PPOA) as a biomarker Biotechnology Reports Mangroves Oil pollution Gene expression Detoxification Polycyclic aromatic hydrocarbons (PAH) Biomarker |
| title | Assessment of oxidative stress response genes in Avicennia marina exposed to oil contamination – Polyphenol oxidase (PPOA) as a biomarker |
| title_full | Assessment of oxidative stress response genes in Avicennia marina exposed to oil contamination – Polyphenol oxidase (PPOA) as a biomarker |
| title_fullStr | Assessment of oxidative stress response genes in Avicennia marina exposed to oil contamination – Polyphenol oxidase (PPOA) as a biomarker |
| title_full_unstemmed | Assessment of oxidative stress response genes in Avicennia marina exposed to oil contamination – Polyphenol oxidase (PPOA) as a biomarker |
| title_short | Assessment of oxidative stress response genes in Avicennia marina exposed to oil contamination – Polyphenol oxidase (PPOA) as a biomarker |
| title_sort | assessment of oxidative stress response genes in avicennia marina exposed to oil contamination polyphenol oxidase ppoa as a biomarker |
| topic | Mangroves Oil pollution Gene expression Detoxification Polycyclic aromatic hydrocarbons (PAH) Biomarker |
| url | http://www.sciencedirect.com/science/article/pii/S2215017X20305725 |
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