Relationships between Agronomic Traits of Moringa Accessions and In Vitro Gas Production Characteristics of a Test Feed Incubated with or without Moringa Plant Leaf Extracts
The use of medicinal plants and their extracts has recently attracted the attention of many researchers as a methane (CH<sub>4</sub>) mitigation strategy. This study evaluated the relationship of agronomic traits of Moringa accessions with in vitro gas production measurements and feed di...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-10-01
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| Series: | Plants |
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| Online Access: | https://www.mdpi.com/2223-7747/11/21/2901 |
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| author | Addisu Endalew Zeru Abubeker Hassen Zeno Apostolides Julius Tjelele |
| author_facet | Addisu Endalew Zeru Abubeker Hassen Zeno Apostolides Julius Tjelele |
| author_sort | Addisu Endalew Zeru |
| collection | DOAJ |
| description | The use of medicinal plants and their extracts has recently attracted the attention of many researchers as a methane (CH<sub>4</sub>) mitigation strategy. This study evaluated the relationship of agronomic traits of Moringa accessions with in vitro gas production measurements and feed digestibility from ruminants. Twelve Moringa accessions were grown at the Roodeplaat experimental site of the Agricultural Research Council in Pretoria, South Africa. Agronomic traits, such as seedling survival rate, leaf yield, canopy and stem diameter, plant height, number of primary branches, plant vigor, greenness, chlorosis, disease and pest incidences were recorded. The leaves were harvested in the fifth month after transplanting to the field. Freeze-dried leaves were extracted with methanol, and their total phenolic and total flavonoid contents were determined. The extract was applied at a dose of 50 mg/kg of dry matter (DM) feed for in vitro gas production studies. Most of the growth and agronomic traits, i.e., seedling survival rate, leaf yield, canopy diameter, plant height, number of primary branches, the score of plant vigor, and greenness, total phenolics and flavonoids were significantly different among the accessions except for stem diameter and chlorosis score. All accession leaf extracts significantly reduced the total gas and CH<sub>4</sub> production compared with the control with equal or higher in vitro organic matter digestibility. Higher CH<sub>4</sub> inhibition was obtained in <i>Moringa oleifera</i> (<i>M. oleifera</i>) A3 (28.4%) and A11 (29.1%), whereas a lower inhibition was recorded in A1 (17.9%) and A2 (18.2%). The total phenolic (0.62) and total flavonoid (0.71) contents as well as most agronomic traits of the accessions were positively correlated with the CH<sub>4</sub> inhibition potential of the accessions. <i>Moringa oleifera</i> accessions A3, A8 and A11 resulted in higher in vitro CH<sub>4</sub> inhibition potential and improved organic matter digestibility of the feed with equal or higher adaptability performances in the field. Thus, there is a possibility of selecting Moringa accessions for higher antimethanogenic activity without compromising the feed digestibility by selecting for higher total phenolics, total flavonoids and agronomic performances traits. There is a need for further study to determine the long-term adaptability of promising accessions in the study area with concurrent antimethanogenesis efficacy when used in the diet of ruminant animals. |
| first_indexed | 2024-03-09T18:44:31Z |
| format | Article |
| id | doaj.art-e40f775dcc3f4e2f9b1d40d7b5d27178 |
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| language | English |
| last_indexed | 2024-03-09T18:44:31Z |
| publishDate | 2022-10-01 |
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| spelling | doaj.art-e40f775dcc3f4e2f9b1d40d7b5d271782023-11-24T06:24:54ZengMDPI AGPlants2223-77472022-10-011121290110.3390/plants11212901Relationships between Agronomic Traits of Moringa Accessions and In Vitro Gas Production Characteristics of a Test Feed Incubated with or without Moringa Plant Leaf ExtractsAddisu Endalew Zeru0Abubeker Hassen1Zeno Apostolides2Julius Tjelele3Department of Animal Science, University of Pretoria, Pretoria 0002, South AfricaDepartment of Animal Science, University of Pretoria, Pretoria 0002, South AfricaDepartment of Biochemistry, Genetics and Microbiology, University of Pretoria, Pretoria 0002, South AfricaRange and Forage Sciences, Agricultural Research Council, Pretoria 0002, South AfricaThe use of medicinal plants and their extracts has recently attracted the attention of many researchers as a methane (CH<sub>4</sub>) mitigation strategy. This study evaluated the relationship of agronomic traits of Moringa accessions with in vitro gas production measurements and feed digestibility from ruminants. Twelve Moringa accessions were grown at the Roodeplaat experimental site of the Agricultural Research Council in Pretoria, South Africa. Agronomic traits, such as seedling survival rate, leaf yield, canopy and stem diameter, plant height, number of primary branches, plant vigor, greenness, chlorosis, disease and pest incidences were recorded. The leaves were harvested in the fifth month after transplanting to the field. Freeze-dried leaves were extracted with methanol, and their total phenolic and total flavonoid contents were determined. The extract was applied at a dose of 50 mg/kg of dry matter (DM) feed for in vitro gas production studies. Most of the growth and agronomic traits, i.e., seedling survival rate, leaf yield, canopy diameter, plant height, number of primary branches, the score of plant vigor, and greenness, total phenolics and flavonoids were significantly different among the accessions except for stem diameter and chlorosis score. All accession leaf extracts significantly reduced the total gas and CH<sub>4</sub> production compared with the control with equal or higher in vitro organic matter digestibility. Higher CH<sub>4</sub> inhibition was obtained in <i>Moringa oleifera</i> (<i>M. oleifera</i>) A3 (28.4%) and A11 (29.1%), whereas a lower inhibition was recorded in A1 (17.9%) and A2 (18.2%). The total phenolic (0.62) and total flavonoid (0.71) contents as well as most agronomic traits of the accessions were positively correlated with the CH<sub>4</sub> inhibition potential of the accessions. <i>Moringa oleifera</i> accessions A3, A8 and A11 resulted in higher in vitro CH<sub>4</sub> inhibition potential and improved organic matter digestibility of the feed with equal or higher adaptability performances in the field. Thus, there is a possibility of selecting Moringa accessions for higher antimethanogenic activity without compromising the feed digestibility by selecting for higher total phenolics, total flavonoids and agronomic performances traits. There is a need for further study to determine the long-term adaptability of promising accessions in the study area with concurrent antimethanogenesis efficacy when used in the diet of ruminant animals.https://www.mdpi.com/2223-7747/11/21/2901methane mitigationorganic matter digestibilityruminantstotal flavonoidstotal gastotal phenolics |
| spellingShingle | Addisu Endalew Zeru Abubeker Hassen Zeno Apostolides Julius Tjelele Relationships between Agronomic Traits of Moringa Accessions and In Vitro Gas Production Characteristics of a Test Feed Incubated with or without Moringa Plant Leaf Extracts Plants methane mitigation organic matter digestibility ruminants total flavonoids total gas total phenolics |
| title | Relationships between Agronomic Traits of Moringa Accessions and In Vitro Gas Production Characteristics of a Test Feed Incubated with or without Moringa Plant Leaf Extracts |
| title_full | Relationships between Agronomic Traits of Moringa Accessions and In Vitro Gas Production Characteristics of a Test Feed Incubated with or without Moringa Plant Leaf Extracts |
| title_fullStr | Relationships between Agronomic Traits of Moringa Accessions and In Vitro Gas Production Characteristics of a Test Feed Incubated with or without Moringa Plant Leaf Extracts |
| title_full_unstemmed | Relationships between Agronomic Traits of Moringa Accessions and In Vitro Gas Production Characteristics of a Test Feed Incubated with or without Moringa Plant Leaf Extracts |
| title_short | Relationships between Agronomic Traits of Moringa Accessions and In Vitro Gas Production Characteristics of a Test Feed Incubated with or without Moringa Plant Leaf Extracts |
| title_sort | relationships between agronomic traits of moringa accessions and in vitro gas production characteristics of a test feed incubated with or without moringa plant leaf extracts |
| topic | methane mitigation organic matter digestibility ruminants total flavonoids total gas total phenolics |
| url | https://www.mdpi.com/2223-7747/11/21/2901 |
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