Assessment of the New Set of Reagents AmpliSens <i>Brucella spp</i>.-FL with Hybridization-Fluorescent Detection
At CRIE, Moscow and RRAPI Microbe designed is the set of reagents for detection of Brucella spp. DNA in biological materials and cultures of microorganisms by means of PCR with hybridization-fluorescent registration of the results. This reagent set, AmpliSens ® Brrucella spp.-FL, is supplied in two...
| Main Authors: | , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | Russian |
| Published: |
Federal Government Health Institution, Russian Research Anti-Plague Institute “Microbe”
2011-06-01
|
| Series: | Проблемы особо опасных инфекций |
| Subjects: | |
| Online Access: | https://journal.microbe.ru/jour/article/view/682 |
| _version_ | 1797220974032060416 |
|---|---|
| author | A. N. Malakhaeva L. V. Sayapina I. S. Barulina I. V. Kasina A. S. Abdrashitova N. A. Osina I. N. Sharova S. B. Yatsyshina N. V. Mayorov L. V. Zaytseva N. P. Mironova |
| author_facet | A. N. Malakhaeva L. V. Sayapina I. S. Barulina I. V. Kasina A. S. Abdrashitova N. A. Osina I. N. Sharova S. B. Yatsyshina N. V. Mayorov L. V. Zaytseva N. P. Mironova |
| author_sort | A. N. Malakhaeva |
| collection | DOAJ |
| description | At CRIE, Moscow and RRAPI Microbe designed is the set of reagents for detection of Brucella spp. DNA in biological materials and cultures of microorganisms by means of PCR with hybridization-fluorescent registration of the results. This reagent set, AmpliSens ® Brrucella spp.-FL, is supplied in two modifications versus the mechanism of the results registration (FRT - real time and FEP - end-point). High diagnostic value of the reagent set is determined in medical trials at L.A. Tarasevich Institute. The set is registered as medical device. |
| first_indexed | 2024-03-08T13:44:54Z |
| format | Article |
| id | doaj.art-e411e30ffa0b42ff80808320141d414c |
| institution | Directory Open Access Journal |
| issn | 0370-1069 2658-719X |
| language | Russian |
| last_indexed | 2024-04-24T12:58:03Z |
| publishDate | 2011-06-01 |
| publisher | Federal Government Health Institution, Russian Research Anti-Plague Institute “Microbe” |
| record_format | Article |
| series | Проблемы особо опасных инфекций |
| spelling | doaj.art-e411e30ffa0b42ff80808320141d414c2024-04-05T16:47:07ZrusFederal Government Health Institution, Russian Research Anti-Plague Institute “Microbe”Проблемы особо опасных инфекций0370-10692658-719X2011-06-0103(109)586010.21055/0370-1069-2011-3(109)-58-60666Assessment of the New Set of Reagents AmpliSens <i>Brucella spp</i>.-FL with Hybridization-Fluorescent DetectionA. N. Malakhaeva0L. V. Sayapina1I. S. Barulina2I. V. Kasina3A. S. Abdrashitova4N. A. Osina5I. N. Sharova6S. B. Yatsyshina7N. V. Mayorov8L. V. Zaytseva9N. P. Mironova10L.A.Tarasevich State Research Institute for Standardization and ControlL.A.Tarasevich State Research Institute for Standardization and ControlL.A.Tarasevich State Research Institute for Standardization and ControlL.A.Tarasevich State Research Institute for Standardization and ControlL.A.Tarasevich State Research Institute for Standardization and ControlRussian Research Anti-Plague Institute MicrobeRussian Research Anti-Plague Institute MicrobeCentral Research Institute of EpidemiologyRussian Research Anti-Plague Institute MicrobeRussian Research Anti-Plague Institute MicrobeRussian Research Anti-Plague Institute MicrobeAt CRIE, Moscow and RRAPI Microbe designed is the set of reagents for detection of Brucella spp. DNA in biological materials and cultures of microorganisms by means of PCR with hybridization-fluorescent registration of the results. This reagent set, AmpliSens ® Brrucella spp.-FL, is supplied in two modifications versus the mechanism of the results registration (FRT - real time and FEP - end-point). High diagnostic value of the reagent set is determined in medical trials at L.A. Tarasevich Institute. The set is registered as medical device.https://journal.microbe.ru/jour/article/view/682brucellosis<i>brucella spp.</i>pcrhybridization-fluorescent registration of the resultsspecific activityspecificityreproducibility |
| spellingShingle | A. N. Malakhaeva L. V. Sayapina I. S. Barulina I. V. Kasina A. S. Abdrashitova N. A. Osina I. N. Sharova S. B. Yatsyshina N. V. Mayorov L. V. Zaytseva N. P. Mironova Assessment of the New Set of Reagents AmpliSens <i>Brucella spp</i>.-FL with Hybridization-Fluorescent Detection Проблемы особо опасных инфекций brucellosis <i>brucella spp.</i> pcr hybridization-fluorescent registration of the results specific activity specificity reproducibility |
| title | Assessment of the New Set of Reagents AmpliSens <i>Brucella spp</i>.-FL with Hybridization-Fluorescent Detection |
| title_full | Assessment of the New Set of Reagents AmpliSens <i>Brucella spp</i>.-FL with Hybridization-Fluorescent Detection |
| title_fullStr | Assessment of the New Set of Reagents AmpliSens <i>Brucella spp</i>.-FL with Hybridization-Fluorescent Detection |
| title_full_unstemmed | Assessment of the New Set of Reagents AmpliSens <i>Brucella spp</i>.-FL with Hybridization-Fluorescent Detection |
| title_short | Assessment of the New Set of Reagents AmpliSens <i>Brucella spp</i>.-FL with Hybridization-Fluorescent Detection |
| title_sort | assessment of the new set of reagents amplisens i brucella spp i fl with hybridization fluorescent detection |
| topic | brucellosis <i>brucella spp.</i> pcr hybridization-fluorescent registration of the results specific activity specificity reproducibility |
| url | https://journal.microbe.ru/jour/article/view/682 |
| work_keys_str_mv | AT anmalakhaeva assessmentofthenewsetofreagentsamplisensibrucellasppiflwithhybridizationfluorescentdetection AT lvsayapina assessmentofthenewsetofreagentsamplisensibrucellasppiflwithhybridizationfluorescentdetection AT isbarulina assessmentofthenewsetofreagentsamplisensibrucellasppiflwithhybridizationfluorescentdetection AT ivkasina assessmentofthenewsetofreagentsamplisensibrucellasppiflwithhybridizationfluorescentdetection AT asabdrashitova assessmentofthenewsetofreagentsamplisensibrucellasppiflwithhybridizationfluorescentdetection AT naosina assessmentofthenewsetofreagentsamplisensibrucellasppiflwithhybridizationfluorescentdetection AT insharova assessmentofthenewsetofreagentsamplisensibrucellasppiflwithhybridizationfluorescentdetection AT sbyatsyshina assessmentofthenewsetofreagentsamplisensibrucellasppiflwithhybridizationfluorescentdetection AT nvmayorov assessmentofthenewsetofreagentsamplisensibrucellasppiflwithhybridizationfluorescentdetection AT lvzaytseva assessmentofthenewsetofreagentsamplisensibrucellasppiflwithhybridizationfluorescentdetection AT npmironova assessmentofthenewsetofreagentsamplisensibrucellasppiflwithhybridizationfluorescentdetection |