Biological nitrification inhibition by root exudates of native species, Hibiscus splendens and Solanum echinatum
Australian native species grow competitively in nutrient limited environments, particularly in nitrogen (N) limited soils; however, the mechanism that enables this is poorly understood. Biological nitrification inhibition (BNI), which is the release of root exudates into the plant rhizosphere to inh...
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| Format: | Article |
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PeerJ Inc.
2018-06-01
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| Online Access: | https://peerj.com/articles/4960.pdf |
| _version_ | 1797424216637702144 |
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| author | Chelsea K. Janke Laura A. Wendling Ryosuke Fujinuma |
| author_facet | Chelsea K. Janke Laura A. Wendling Ryosuke Fujinuma |
| author_sort | Chelsea K. Janke |
| collection | DOAJ |
| description | Australian native species grow competitively in nutrient limited environments, particularly in nitrogen (N) limited soils; however, the mechanism that enables this is poorly understood. Biological nitrification inhibition (BNI), which is the release of root exudates into the plant rhizosphere to inhibit the nitrification process, is a hypothesized adaptive mechanism for maximizing N uptake. To date, few studies have investigated the temporal pattern and components of root exudates by Australian native plant species for BNI. This study examined root exudates from two Australian native species, Hibiscus splendens and Solanum echinatum, and contrasted with exudates of Sorghum bicolor, a plant widely demonstrated to exhibit BNI capacity. Root exudates were collected from plants at two, four, and six weeks after transplanting to solution culture. Root exudates contained three types of organic acids (OAs), oxalic, citric and succinic acids, regardless of the species. However, the two Australian natives species released larger amount of OAs in earlier development stages than S. bicolor. The total quantity of these OAs released per unit root dry mass was also seven-ten times greater for Australian native plant species compared to S. bicolor. The root exudates significantly inhibited nitrification activity over six weeks’ growth in a potential nitrification assay, with S. echinatum (ca. 81% inhibition) > S. bicolor (ca. 80% inhibition) > H. splendens (ca. 78% inhibition). The narrow range of BNI capacity in the study plants limited the determination of a relationship between OAs and BNI; however, a lack of correlation between individual OAs and inhibition of nitrification suggests OAs may not directly contribute to BNI. These results indicate that Australian native species generate a strongly N conserving environment within the rhizosphere up to six weeks after germination, establishing a competitive advantage in severely N limited environments. |
| first_indexed | 2024-03-09T07:59:01Z |
| format | Article |
| id | doaj.art-e4152d81d0d64763bbf584dd60ab61c6 |
| institution | Directory Open Access Journal |
| issn | 2167-8359 |
| language | English |
| last_indexed | 2024-03-09T07:59:01Z |
| publishDate | 2018-06-01 |
| publisher | PeerJ Inc. |
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| spelling | doaj.art-e4152d81d0d64763bbf584dd60ab61c62023-12-03T00:50:40ZengPeerJ Inc.PeerJ2167-83592018-06-016e496010.7717/peerj.4960Biological nitrification inhibition by root exudates of native species, Hibiscus splendens and Solanum echinatumChelsea K. Janke0Laura A. Wendling1Ryosuke Fujinuma2School of Agriculture and Food Sciences, University of Queensland, AustraliaSchool of Agriculture and Food Sciences, University of Queensland, AustraliaSchool of Agriculture and Food Sciences, University of Queensland, AustraliaAustralian native species grow competitively in nutrient limited environments, particularly in nitrogen (N) limited soils; however, the mechanism that enables this is poorly understood. Biological nitrification inhibition (BNI), which is the release of root exudates into the plant rhizosphere to inhibit the nitrification process, is a hypothesized adaptive mechanism for maximizing N uptake. To date, few studies have investigated the temporal pattern and components of root exudates by Australian native plant species for BNI. This study examined root exudates from two Australian native species, Hibiscus splendens and Solanum echinatum, and contrasted with exudates of Sorghum bicolor, a plant widely demonstrated to exhibit BNI capacity. Root exudates were collected from plants at two, four, and six weeks after transplanting to solution culture. Root exudates contained three types of organic acids (OAs), oxalic, citric and succinic acids, regardless of the species. However, the two Australian natives species released larger amount of OAs in earlier development stages than S. bicolor. The total quantity of these OAs released per unit root dry mass was also seven-ten times greater for Australian native plant species compared to S. bicolor. The root exudates significantly inhibited nitrification activity over six weeks’ growth in a potential nitrification assay, with S. echinatum (ca. 81% inhibition) > S. bicolor (ca. 80% inhibition) > H. splendens (ca. 78% inhibition). The narrow range of BNI capacity in the study plants limited the determination of a relationship between OAs and BNI; however, a lack of correlation between individual OAs and inhibition of nitrification suggests OAs may not directly contribute to BNI. These results indicate that Australian native species generate a strongly N conserving environment within the rhizosphere up to six weeks after germination, establishing a competitive advantage in severely N limited environments.https://peerj.com/articles/4960.pdfNative speciesNutrient cyclingNitrogenBiological nitrification inhibitionEcologyRoot exudates |
| spellingShingle | Chelsea K. Janke Laura A. Wendling Ryosuke Fujinuma Biological nitrification inhibition by root exudates of native species, Hibiscus splendens and Solanum echinatum PeerJ Native species Nutrient cycling Nitrogen Biological nitrification inhibition Ecology Root exudates |
| title | Biological nitrification inhibition by root exudates of native species, Hibiscus splendens and Solanum echinatum |
| title_full | Biological nitrification inhibition by root exudates of native species, Hibiscus splendens and Solanum echinatum |
| title_fullStr | Biological nitrification inhibition by root exudates of native species, Hibiscus splendens and Solanum echinatum |
| title_full_unstemmed | Biological nitrification inhibition by root exudates of native species, Hibiscus splendens and Solanum echinatum |
| title_short | Biological nitrification inhibition by root exudates of native species, Hibiscus splendens and Solanum echinatum |
| title_sort | biological nitrification inhibition by root exudates of native species hibiscus splendens and solanum echinatum |
| topic | Native species Nutrient cycling Nitrogen Biological nitrification inhibition Ecology Root exudates |
| url | https://peerj.com/articles/4960.pdf |
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