Evaluation of New Generation Sequencing (NGS)-Based Somatic Gene Variations and Real-Time Polymerase Chain Reaction (PCR)-Based Gene Fusions in Elderly and Young Acute Leukemia Patients: A Retrospective View
Malignant diseases occurring in elderly patients follow a different course from younger patients and show different genetic structures. Therefore, in this retrospective study, the somatic gene variant profile and fusion gene profiles of elderly and young acute leukemia patients were determined to dr...
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| Format: | Article |
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MDPI AG
2024-01-01
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| Series: | Journal of Personalized Medicine |
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| Online Access: | https://www.mdpi.com/2075-4426/14/2/140 |
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| author | İbrahim Halil Erdoğdu Seda Örenay-Boyacıoğlu Olcay Boyacıoğlu Nesibe Kahraman-Çetin Füruzan Kacar-Döger İrfan Yavaşoğlu Ali Zahit Bolaman |
| author_facet | İbrahim Halil Erdoğdu Seda Örenay-Boyacıoğlu Olcay Boyacıoğlu Nesibe Kahraman-Çetin Füruzan Kacar-Döger İrfan Yavaşoğlu Ali Zahit Bolaman |
| author_sort | İbrahim Halil Erdoğdu |
| collection | DOAJ |
| description | Malignant diseases occurring in elderly patients follow a different course from younger patients and show different genetic structures. Therefore, in this retrospective study, the somatic gene variant profile and fusion gene profiles of elderly and young acute leukemia patients were determined to draw attention to the existing genetic difference, and the results were compared. In this study, the records of 204 acute leukemia patients aged 18+ who were referred to the Molecular Pathology Laboratory from the Hematology Clinic between 2018 and 2022 were reviewed retrospectively. Fusion gene detection in patients was performed with the HemaVision<sup>®</sup>-28Q Panel. The NGS Myeloid Neoplasms Panel was conducted using the MiniSEQ NGS platform according to the manufacturer’s protocol. When all cases are evaluated together, the most frequently diagnosed acute leukemia is acute myeloid leukemia (85.8%). Both groups had a similar fusion gene profile; however, the fusion burden was higher in the elderly group. When the groups were evaluated in terms of somatic gene variations, there were differences between the groups, and the variation load was higher in the elderly group. Considering the different somatic gene variation profiles, it is understood that the genetic structure of tumor cells is different in elderly patients compared to young cases. |
| first_indexed | 2024-03-07T22:26:11Z |
| format | Article |
| id | doaj.art-e41659ee28f54838a46a4e6c268da1e1 |
| institution | Directory Open Access Journal |
| issn | 2075-4426 |
| language | English |
| last_indexed | 2024-03-07T22:26:11Z |
| publishDate | 2024-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Journal of Personalized Medicine |
| spelling | doaj.art-e41659ee28f54838a46a4e6c268da1e12024-02-23T15:23:39ZengMDPI AGJournal of Personalized Medicine2075-44262024-01-0114214010.3390/jpm14020140Evaluation of New Generation Sequencing (NGS)-Based Somatic Gene Variations and Real-Time Polymerase Chain Reaction (PCR)-Based Gene Fusions in Elderly and Young Acute Leukemia Patients: A Retrospective Viewİbrahim Halil Erdoğdu0Seda Örenay-Boyacıoğlu1Olcay Boyacıoğlu2Nesibe Kahraman-Çetin3Füruzan Kacar-Döger4İrfan Yavaşoğlu5Ali Zahit Bolaman6Department of Molecular Pathology, Faculty of Medicine, Aydin Adnan Menderes University, Aydin 09010, TürkiyeDepartment of Medical Genetics, Faculty of Medicine, Aydin Adnan Menderes University, Aydin 09010, TürkiyeFaculty of Engineering, Aydin Adnan Menderes University, Aydin 09010, TürkiyeDepartment of Molecular Pathology, Faculty of Medicine, Aydin Adnan Menderes University, Aydin 09010, TürkiyeDepartment of Molecular Pathology, Faculty of Medicine, Aydin Adnan Menderes University, Aydin 09010, TürkiyeDepartment of Hematology, Faculty of Medicine, Aydin Adnan Menderes University, Aydin 09010, TürkiyeDepartment of Hematology, Faculty of Medicine, Aydin Adnan Menderes University, Aydin 09010, TürkiyeMalignant diseases occurring in elderly patients follow a different course from younger patients and show different genetic structures. Therefore, in this retrospective study, the somatic gene variant profile and fusion gene profiles of elderly and young acute leukemia patients were determined to draw attention to the existing genetic difference, and the results were compared. In this study, the records of 204 acute leukemia patients aged 18+ who were referred to the Molecular Pathology Laboratory from the Hematology Clinic between 2018 and 2022 were reviewed retrospectively. Fusion gene detection in patients was performed with the HemaVision<sup>®</sup>-28Q Panel. The NGS Myeloid Neoplasms Panel was conducted using the MiniSEQ NGS platform according to the manufacturer’s protocol. When all cases are evaluated together, the most frequently diagnosed acute leukemia is acute myeloid leukemia (85.8%). Both groups had a similar fusion gene profile; however, the fusion burden was higher in the elderly group. When the groups were evaluated in terms of somatic gene variations, there were differences between the groups, and the variation load was higher in the elderly group. Considering the different somatic gene variation profiles, it is understood that the genetic structure of tumor cells is different in elderly patients compared to young cases.https://www.mdpi.com/2075-4426/14/2/140geriatricsacute leukemiagene variationsgene fusionsNGS |
| spellingShingle | İbrahim Halil Erdoğdu Seda Örenay-Boyacıoğlu Olcay Boyacıoğlu Nesibe Kahraman-Çetin Füruzan Kacar-Döger İrfan Yavaşoğlu Ali Zahit Bolaman Evaluation of New Generation Sequencing (NGS)-Based Somatic Gene Variations and Real-Time Polymerase Chain Reaction (PCR)-Based Gene Fusions in Elderly and Young Acute Leukemia Patients: A Retrospective View Journal of Personalized Medicine geriatrics acute leukemia gene variations gene fusions NGS |
| title | Evaluation of New Generation Sequencing (NGS)-Based Somatic Gene Variations and Real-Time Polymerase Chain Reaction (PCR)-Based Gene Fusions in Elderly and Young Acute Leukemia Patients: A Retrospective View |
| title_full | Evaluation of New Generation Sequencing (NGS)-Based Somatic Gene Variations and Real-Time Polymerase Chain Reaction (PCR)-Based Gene Fusions in Elderly and Young Acute Leukemia Patients: A Retrospective View |
| title_fullStr | Evaluation of New Generation Sequencing (NGS)-Based Somatic Gene Variations and Real-Time Polymerase Chain Reaction (PCR)-Based Gene Fusions in Elderly and Young Acute Leukemia Patients: A Retrospective View |
| title_full_unstemmed | Evaluation of New Generation Sequencing (NGS)-Based Somatic Gene Variations and Real-Time Polymerase Chain Reaction (PCR)-Based Gene Fusions in Elderly and Young Acute Leukemia Patients: A Retrospective View |
| title_short | Evaluation of New Generation Sequencing (NGS)-Based Somatic Gene Variations and Real-Time Polymerase Chain Reaction (PCR)-Based Gene Fusions in Elderly and Young Acute Leukemia Patients: A Retrospective View |
| title_sort | evaluation of new generation sequencing ngs based somatic gene variations and real time polymerase chain reaction pcr based gene fusions in elderly and young acute leukemia patients a retrospective view |
| topic | geriatrics acute leukemia gene variations gene fusions NGS |
| url | https://www.mdpi.com/2075-4426/14/2/140 |
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