Vkorc1 gene polymorphisms confer resistance to anticoagulant rodenticide in Turkish rats
Mutations in Exon 1, 2 and 3 of the vitamin K epoxide reductase complex subunit 1 (Vkorc1) gene are known to lead to anticoagulant rodenticide resistance. In order to investigate their putative resistance in rodenticides, we studied the genetic profile of the Vkorc1 gene in Turkish black rats (Rattu...
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PeerJ Inc.
2023-05-01
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| author | Nuri Yiğit Mustafa T. Duman Derya Çetintürk Fulya Saygılı-Yiğit Ercüment Çolak Reyhan Çolak |
| author_facet | Nuri Yiğit Mustafa T. Duman Derya Çetintürk Fulya Saygılı-Yiğit Ercüment Çolak Reyhan Çolak |
| author_sort | Nuri Yiğit |
| collection | DOAJ |
| description | Mutations in Exon 1, 2 and 3 of the vitamin K epoxide reductase complex subunit 1 (Vkorc1) gene are known to lead to anticoagulant rodenticide resistance. In order to investigate their putative resistance in rodenticides, we studied the genetic profile of the Vkorc1 gene in Turkish black rats (Rattus rattus) and brown rats (Rattus norvegicus). In this context, previously recorded Ala21Thr mutation (R. rattus) in Exon 1 region, Ile90Leu mutation (R. rattus, R. norvegicus) in Exon 2 region and Leu120Gln mutation (R. norvegicus) in Exon 3 region were identified as “missense mutations” causing amino acid changes. Ala21Thr mutation was first detected in one specimen of Turkish black rat despite the uncertainty of its relevance to resistance. Ile90Leu mutation accepted as neutral variant was detected in most of black rat specimens. Leu120Gln mutation related to anticoagulant rodenticide resistance was found in only one brown rat specimen. Furthermore, Ser74Asn, Gln77Pro (black rat) and Ser79Pro (brown rat) mutations that cause amino acid changes in the Exon 2 region but unclear whether they cause resistance were identified. In addition, “silent mutations” which do not cause amino acid changes were also defined; these mutations were Arg12Arg mutation in Exon 1 region, His68His, Ser81Ser, Ile82Ile and Leu94Leu mutations in Exon 2 region and Ile107Ile, Thr137Thr, Ala143Ala and Gln152Gln mutations in Exon 3 region. These silent mutations were found in both species except for Ser81Ser which was determined in only brown rats. |
| first_indexed | 2024-03-09T06:28:19Z |
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| id | doaj.art-e41823959f0c42fbb04c2a906688dc4b |
| institution | Directory Open Access Journal |
| issn | 2167-8359 |
| language | English |
| last_indexed | 2024-03-09T06:28:19Z |
| publishDate | 2023-05-01 |
| publisher | PeerJ Inc. |
| record_format | Article |
| series | PeerJ |
| spelling | doaj.art-e41823959f0c42fbb04c2a906688dc4b2023-12-03T11:13:48ZengPeerJ Inc.PeerJ2167-83592023-05-0111e1505510.7717/peerj.15055Vkorc1 gene polymorphisms confer resistance to anticoagulant rodenticide in Turkish ratsNuri Yiğit0Mustafa T. Duman1Derya Çetintürk2Fulya Saygılı-Yiğit3Ercüment Çolak4Reyhan Çolak5Biology Department/Faculty of Science, Ankara University, Ankara, TurkeyBiology Department/Faculty of Science, Ankara University, Ankara, TurkeyBiology Department/Faculty of Science, Ankara University, Ankara, TurkeyBiotechnology Department/Faculty of Arts and Sciences, Niğde Ömer Halisdemir University, Niğde, TurkeyBiology Department/Faculty of Science, Ankara University, Ankara, TurkeyBiology Department/Faculty of Science, Ankara University, Ankara, TurkeyMutations in Exon 1, 2 and 3 of the vitamin K epoxide reductase complex subunit 1 (Vkorc1) gene are known to lead to anticoagulant rodenticide resistance. In order to investigate their putative resistance in rodenticides, we studied the genetic profile of the Vkorc1 gene in Turkish black rats (Rattus rattus) and brown rats (Rattus norvegicus). In this context, previously recorded Ala21Thr mutation (R. rattus) in Exon 1 region, Ile90Leu mutation (R. rattus, R. norvegicus) in Exon 2 region and Leu120Gln mutation (R. norvegicus) in Exon 3 region were identified as “missense mutations” causing amino acid changes. Ala21Thr mutation was first detected in one specimen of Turkish black rat despite the uncertainty of its relevance to resistance. Ile90Leu mutation accepted as neutral variant was detected in most of black rat specimens. Leu120Gln mutation related to anticoagulant rodenticide resistance was found in only one brown rat specimen. Furthermore, Ser74Asn, Gln77Pro (black rat) and Ser79Pro (brown rat) mutations that cause amino acid changes in the Exon 2 region but unclear whether they cause resistance were identified. In addition, “silent mutations” which do not cause amino acid changes were also defined; these mutations were Arg12Arg mutation in Exon 1 region, His68His, Ser81Ser, Ile82Ile and Leu94Leu mutations in Exon 2 region and Ile107Ile, Thr137Thr, Ala143Ala and Gln152Gln mutations in Exon 3 region. These silent mutations were found in both species except for Ser81Ser which was determined in only brown rats.https://peerj.com/articles/15055.pdfVkorc1Anticoagulant resistanceMutationBlack ratBrown rat |
| spellingShingle | Nuri Yiğit Mustafa T. Duman Derya Çetintürk Fulya Saygılı-Yiğit Ercüment Çolak Reyhan Çolak Vkorc1 gene polymorphisms confer resistance to anticoagulant rodenticide in Turkish rats PeerJ Vkorc1 Anticoagulant resistance Mutation Black rat Brown rat |
| title | Vkorc1 gene polymorphisms confer resistance to anticoagulant rodenticide in Turkish rats |
| title_full | Vkorc1 gene polymorphisms confer resistance to anticoagulant rodenticide in Turkish rats |
| title_fullStr | Vkorc1 gene polymorphisms confer resistance to anticoagulant rodenticide in Turkish rats |
| title_full_unstemmed | Vkorc1 gene polymorphisms confer resistance to anticoagulant rodenticide in Turkish rats |
| title_short | Vkorc1 gene polymorphisms confer resistance to anticoagulant rodenticide in Turkish rats |
| title_sort | vkorc1 gene polymorphisms confer resistance to anticoagulant rodenticide in turkish rats |
| topic | Vkorc1 Anticoagulant resistance Mutation Black rat Brown rat |
| url | https://peerj.com/articles/15055.pdf |
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