Production and some properties of crude alkaline proteases of indigenous Central Amazonian rhizobia strains
Two rhizobia strains isolated from soils of the Central Amazonian floodplain produced appreciable quantities of crude alkaline protease extracts with inexpensive carbon and nitrogen sources. These protease crude extracts were optimally active at pH 9.0-11.0. The optimum temperatures were 35 ºC for R...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Instituto de Tecnologia do Paraná (Tecpar)
2010-10-01
|
| Series: | Brazilian Archives of Biology and Technology |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000500024 |
| _version_ | 1819053128104804352 |
|---|---|
| author | Arlem Nascimento de Oliveira Luiz Antonio de Oliveira Jerusa Souza Andrade |
| author_facet | Arlem Nascimento de Oliveira Luiz Antonio de Oliveira Jerusa Souza Andrade |
| author_sort | Arlem Nascimento de Oliveira |
| collection | DOAJ |
| description | Two rhizobia strains isolated from soils of the Central Amazonian floodplain produced appreciable quantities of crude alkaline protease extracts with inexpensive carbon and nitrogen sources. These protease crude extracts were optimally active at pH 9.0-11.0. The optimum temperatures were 35 ºC for Rhizobium sp. strain R-986 and 55 ºC for Bradyrhizobium sp. strain R-993. Protease activities in the crude extracts were enhanced in the presence of 5 mM metal ions, such as Na+, Ca2+, Mg2+ and Mn2+. Rhizobia proteases were strongly inhibited by PMSF, a serine-protease inhibitor. The enzymes were active in the presence of surfactants (SDS and Triton X-100) and stable in oxidizing (H2O2) and reducing agents (β-mercaptoethanol), and organic solvents (acetone, hexane, methanol, 1-propanol and toluene).<br>Duas estirpes de rizóbia isoladas de solos de várzea da Amazônia Central produziram grandes quantidades de proteases alcalinas extracelulares, usando fontes baratas de carbono e nitrogênio. Os extratos brutos de proteases foram ativos em pH 9,0-11,0. As temperaturas ótimas foram de 35 ºC para a enzima do Rhizobium R-986 e de 55 ºC para a do Bradyrhizobium R-993. As atividades proteolíticas aumentaram na presença de 5 mM dos íons Na+, Ca2+ , Mg2+ e Mn2+ . As proteases secretadas pelos rizóbios foram fortemente inibidas por PMSF, um inibidor de serina protease. As enzimas foram ativas na presença de surfactantes (SDS e Triton X-100), e estáveis na presença de agentes oxidantes (H2O2) e redutores (β-mercaptoetanol) e solventes orgânicos (acetona, hexano, metanol, 1-propanol e tolueno). |
| first_indexed | 2024-12-21T12:30:48Z |
| format | Article |
| id | doaj.art-e41c081bffd446b9a3d81cde6f590ec8 |
| institution | Directory Open Access Journal |
| issn | 1516-8913 1678-4324 |
| language | English |
| last_indexed | 2024-12-21T12:30:48Z |
| publishDate | 2010-10-01 |
| publisher | Instituto de Tecnologia do Paraná (Tecpar) |
| record_format | Article |
| series | Brazilian Archives of Biology and Technology |
| spelling | doaj.art-e41c081bffd446b9a3d81cde6f590ec82022-12-21T19:04:03ZengInstituto de Tecnologia do Paraná (Tecpar)Brazilian Archives of Biology and Technology1516-89131678-43242010-10-015351185119510.1590/S1516-89132010000500024Production and some properties of crude alkaline proteases of indigenous Central Amazonian rhizobia strainsArlem Nascimento de OliveiraLuiz Antonio de OliveiraJerusa Souza AndradeTwo rhizobia strains isolated from soils of the Central Amazonian floodplain produced appreciable quantities of crude alkaline protease extracts with inexpensive carbon and nitrogen sources. These protease crude extracts were optimally active at pH 9.0-11.0. The optimum temperatures were 35 ºC for Rhizobium sp. strain R-986 and 55 ºC for Bradyrhizobium sp. strain R-993. Protease activities in the crude extracts were enhanced in the presence of 5 mM metal ions, such as Na+, Ca2+, Mg2+ and Mn2+. Rhizobia proteases were strongly inhibited by PMSF, a serine-protease inhibitor. The enzymes were active in the presence of surfactants (SDS and Triton X-100) and stable in oxidizing (H2O2) and reducing agents (β-mercaptoethanol), and organic solvents (acetone, hexane, methanol, 1-propanol and toluene).<br>Duas estirpes de rizóbia isoladas de solos de várzea da Amazônia Central produziram grandes quantidades de proteases alcalinas extracelulares, usando fontes baratas de carbono e nitrogênio. Os extratos brutos de proteases foram ativos em pH 9,0-11,0. As temperaturas ótimas foram de 35 ºC para a enzima do Rhizobium R-986 e de 55 ºC para a do Bradyrhizobium R-993. As atividades proteolíticas aumentaram na presença de 5 mM dos íons Na+, Ca2+ , Mg2+ e Mn2+ . As proteases secretadas pelos rizóbios foram fortemente inibidas por PMSF, um inibidor de serina protease. As enzimas foram ativas na presença de surfactantes (SDS e Triton X-100), e estáveis na presença de agentes oxidantes (H2O2) e redutores (β-mercaptoetanol) e solventes orgânicos (acetona, hexano, metanol, 1-propanol e tolueno).http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000500024RhizobiumBradyrhizobiumenzymatic activitiesorganic surfactants |
| spellingShingle | Arlem Nascimento de Oliveira Luiz Antonio de Oliveira Jerusa Souza Andrade Production and some properties of crude alkaline proteases of indigenous Central Amazonian rhizobia strains Brazilian Archives of Biology and Technology Rhizobium Bradyrhizobium enzymatic activities organic surfactants |
| title | Production and some properties of crude alkaline proteases of indigenous Central Amazonian rhizobia strains |
| title_full | Production and some properties of crude alkaline proteases of indigenous Central Amazonian rhizobia strains |
| title_fullStr | Production and some properties of crude alkaline proteases of indigenous Central Amazonian rhizobia strains |
| title_full_unstemmed | Production and some properties of crude alkaline proteases of indigenous Central Amazonian rhizobia strains |
| title_short | Production and some properties of crude alkaline proteases of indigenous Central Amazonian rhizobia strains |
| title_sort | production and some properties of crude alkaline proteases of indigenous central amazonian rhizobia strains |
| topic | Rhizobium Bradyrhizobium enzymatic activities organic surfactants |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000500024 |
| work_keys_str_mv | AT arlemnascimentodeoliveira productionandsomepropertiesofcrudealkalineproteasesofindigenouscentralamazonianrhizobiastrains AT luizantoniodeoliveira productionandsomepropertiesofcrudealkalineproteasesofindigenouscentralamazonianrhizobiastrains AT jerusasouzaandrade productionandsomepropertiesofcrudealkalineproteasesofindigenouscentralamazonianrhizobiastrains |