Silver Nanoparticles: A Promising Antifungal Agent against the Growth and Biofilm Formation of the Emergent <i>Candida auris</i>
<i>Candida auris</i> is a globally-emerging pathogen that is correlated to nosocomial infections and high mortality rates, causing major outbreaks in hospitals and serious public health concerns worldwide. This study investigated the antifungal activity of silver nanoparticles (AgNPs) on...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-07-01
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| Series: | Journal of Fungi |
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| Online Access: | https://www.mdpi.com/2309-608X/8/7/744 |
| _version_ | 1797433424875618304 |
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| author | Reem AlJindan Doaa M. AlEraky |
| author_facet | Reem AlJindan Doaa M. AlEraky |
| author_sort | Reem AlJindan |
| collection | DOAJ |
| description | <i>Candida auris</i> is a globally-emerging pathogen that is correlated to nosocomial infections and high mortality rates, causing major outbreaks in hospitals and serious public health concerns worldwide. This study investigated the antifungal activity of silver nanoparticles (AgNPs) on clinical isolates of <i>C. auris.</i> A total of eight clinical isolates were collected from blood, urine, ear swab, and groin. <i>C. auris</i> was confirmed by MALDI-TOF MS, and gene sequencing. All isolates confirmed as <i>C. auris</i> were subjected to antimicrobial agents, including amphotericin B, fluconazole, caspofungin, voriconazole, micafungin, and flucytosine. A serial dilution of a silver nanoparticles solution was prepared to test antifungal susceptibility testing under planktonic conditions. Moreover, an antibiofilm activity assay was determined using a colony-forming assay and a cell viability assay by a live–dead yeast kit. Significant antifungal and antibiofilm activity of AgNPs was detected against all isolates; MIC was <6.25 μg/mL, the range of MFC was from 6.25 to 12.5 μg/mL for all isolates, and the highest value of IC<sub>50</sub> was 3.2 μg/mL. Silver nanomaterials could represent a possible antimicrobial agent to prevent outbreaks caused by <i>C. auris</i> infections. |
| first_indexed | 2024-03-09T10:16:50Z |
| format | Article |
| id | doaj.art-e41c8517f9eb433aa89802bb1571feb9 |
| institution | Directory Open Access Journal |
| issn | 2309-608X |
| language | English |
| last_indexed | 2024-03-09T10:16:50Z |
| publishDate | 2022-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Journal of Fungi |
| spelling | doaj.art-e41c8517f9eb433aa89802bb1571feb92023-12-01T22:20:14ZengMDPI AGJournal of Fungi2309-608X2022-07-018774410.3390/jof8070744Silver Nanoparticles: A Promising Antifungal Agent against the Growth and Biofilm Formation of the Emergent <i>Candida auris</i>Reem AlJindan0Doaa M. AlEraky1Department of Microbiology, College of Medicine, Imam Abdulrahman Bin Faisal University, Dammam P.O. Box 1982, Saudi ArabiaDepartment of Biomedical Dental Science, Microbiology and Immunology, College of Dentistry, Imam Abdulrahman Bin Faisal University, Dammam P.O. Box 1982, Saudi Arabia<i>Candida auris</i> is a globally-emerging pathogen that is correlated to nosocomial infections and high mortality rates, causing major outbreaks in hospitals and serious public health concerns worldwide. This study investigated the antifungal activity of silver nanoparticles (AgNPs) on clinical isolates of <i>C. auris.</i> A total of eight clinical isolates were collected from blood, urine, ear swab, and groin. <i>C. auris</i> was confirmed by MALDI-TOF MS, and gene sequencing. All isolates confirmed as <i>C. auris</i> were subjected to antimicrobial agents, including amphotericin B, fluconazole, caspofungin, voriconazole, micafungin, and flucytosine. A serial dilution of a silver nanoparticles solution was prepared to test antifungal susceptibility testing under planktonic conditions. Moreover, an antibiofilm activity assay was determined using a colony-forming assay and a cell viability assay by a live–dead yeast kit. Significant antifungal and antibiofilm activity of AgNPs was detected against all isolates; MIC was <6.25 μg/mL, the range of MFC was from 6.25 to 12.5 μg/mL for all isolates, and the highest value of IC<sub>50</sub> was 3.2 μg/mL. Silver nanomaterials could represent a possible antimicrobial agent to prevent outbreaks caused by <i>C. auris</i> infections.https://www.mdpi.com/2309-608X/8/7/744<i>Candida auris</i>emerging pathogenantifungal susceptibility testinganti-biofilm formation |
| spellingShingle | Reem AlJindan Doaa M. AlEraky Silver Nanoparticles: A Promising Antifungal Agent against the Growth and Biofilm Formation of the Emergent <i>Candida auris</i> Journal of Fungi <i>Candida auris</i> emerging pathogen antifungal susceptibility testing anti-biofilm formation |
| title | Silver Nanoparticles: A Promising Antifungal Agent against the Growth and Biofilm Formation of the Emergent <i>Candida auris</i> |
| title_full | Silver Nanoparticles: A Promising Antifungal Agent against the Growth and Biofilm Formation of the Emergent <i>Candida auris</i> |
| title_fullStr | Silver Nanoparticles: A Promising Antifungal Agent against the Growth and Biofilm Formation of the Emergent <i>Candida auris</i> |
| title_full_unstemmed | Silver Nanoparticles: A Promising Antifungal Agent against the Growth and Biofilm Formation of the Emergent <i>Candida auris</i> |
| title_short | Silver Nanoparticles: A Promising Antifungal Agent against the Growth and Biofilm Formation of the Emergent <i>Candida auris</i> |
| title_sort | silver nanoparticles a promising antifungal agent against the growth and biofilm formation of the emergent i candida auris i |
| topic | <i>Candida auris</i> emerging pathogen antifungal susceptibility testing anti-biofilm formation |
| url | https://www.mdpi.com/2309-608X/8/7/744 |
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