Chemical Composition, Antioxidant Activity and Cytocompatibility of Polyphenolic Compounds Extracted from Food Industry Apple Waste: Potential in Biomedical Application
Apple pomace (AP) from the food industry is a mixture of different fractions containing bioactive polyphenolic compounds. This study provides a systematic approach toward the recovery and evaluation of the physiochemical and biological properties of polyphenolic compounds from AP. We studied subcrit...
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2023-01-01
|
| Series: | Molecules |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1420-3049/28/2/675 |
| _version_ | 1797438350512095232 |
|---|---|
| author | Parinaz Hobbi Oseweuba Valentine Okoro Maryam Hajiabbas Masoud Hamidi Lei Nie Véronique Megalizzi Paul Musonge Gianina Dodi Amin Shavandi |
| author_facet | Parinaz Hobbi Oseweuba Valentine Okoro Maryam Hajiabbas Masoud Hamidi Lei Nie Véronique Megalizzi Paul Musonge Gianina Dodi Amin Shavandi |
| author_sort | Parinaz Hobbi |
| collection | DOAJ |
| description | Apple pomace (AP) from the food industry is a mixture of different fractions containing bioactive polyphenolic compounds. This study provides a systematic approach toward the recovery and evaluation of the physiochemical and biological properties of polyphenolic compounds from AP. We studied subcritical water extraction (SCW) and solvent extraction with ethanol from four different AP fractions of pulp, peel, seed, core, and stem (A), peel (B), seed and core (C), and pulp and peel (D). The subcritical water method at the optimum condition resulted in total polyphenolic compounds (TPC) of 39.08 ± 1.10 mg GAE per g of AP on a dry basis compared to the ethanol extraction with TPC content of 10.78 ± 0.94 mg GAE/g db. Phloridzin, chlorogenic acid, and quercetin were the main identified polyphenolics in the AP fractions using HPLC. DPPH radical scavenging activity of fraction B and subcritical water (SW) extracts showed comparable activity to ascorbic acid while all ethanolic extracts were cytocompatible toward human fibroblast (3T3-L1) and salivary gland acinar cells (NS-SV-AC). Our results indicated that AP is a rich source of polyphenolics with the potential for biomedical applications. |
| first_indexed | 2024-03-09T11:36:32Z |
| format | Article |
| id | doaj.art-e41d192f95414a19ab52fac1cc0d0b58 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-09T11:36:32Z |
| publishDate | 2023-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-e41d192f95414a19ab52fac1cc0d0b582023-11-30T23:43:11ZengMDPI AGMolecules1420-30492023-01-0128267510.3390/molecules28020675Chemical Composition, Antioxidant Activity and Cytocompatibility of Polyphenolic Compounds Extracted from Food Industry Apple Waste: Potential in Biomedical ApplicationParinaz Hobbi0Oseweuba Valentine Okoro1Maryam Hajiabbas2Masoud Hamidi3Lei Nie4Véronique Megalizzi5Paul Musonge6Gianina Dodi7Amin Shavandi8École Polytechnique de Bruxelles, Université Libre de Bruxelles (ULB), 3BIO-BioMatter, Avenue F.D. Roosevelt, 50-CP 165/61, B-1050 Brussels, BelgiumÉcole Polytechnique de Bruxelles, Université Libre de Bruxelles (ULB), 3BIO-BioMatter, Avenue F.D. Roosevelt, 50-CP 165/61, B-1050 Brussels, BelgiumÉcole Polytechnique de Bruxelles, Université Libre de Bruxelles (ULB), 3BIO-BioMatter, Avenue F.D. Roosevelt, 50-CP 165/61, B-1050 Brussels, BelgiumÉcole Polytechnique de Bruxelles, Université Libre de Bruxelles (ULB), 3BIO-BioMatter, Avenue F.D. Roosevelt, 50-CP 165/61, B-1050 Brussels, BelgiumCollege of Life Sciences, Xinyang Normal University (XYNU), Xinyang 464000, ChinaPharmacognosy, Bioanalysis & Drug Discovery Unit, Faculty of Pharmacy, Université Libre de Bruxelles, B-1070 Brussels, BelgiumInstitute of Systems Science, Durban University of Technology, Durban 4000, South AfricaAdvanced Centre for Research-Development in Experimental Medicine, Grigore T. Popa University of Medicine and Pharmacy of Iasi, 700115 Iasi, RomaniaÉcole Polytechnique de Bruxelles, Université Libre de Bruxelles (ULB), 3BIO-BioMatter, Avenue F.D. Roosevelt, 50-CP 165/61, B-1050 Brussels, BelgiumApple pomace (AP) from the food industry is a mixture of different fractions containing bioactive polyphenolic compounds. This study provides a systematic approach toward the recovery and evaluation of the physiochemical and biological properties of polyphenolic compounds from AP. We studied subcritical water extraction (SCW) and solvent extraction with ethanol from four different AP fractions of pulp, peel, seed, core, and stem (A), peel (B), seed and core (C), and pulp and peel (D). The subcritical water method at the optimum condition resulted in total polyphenolic compounds (TPC) of 39.08 ± 1.10 mg GAE per g of AP on a dry basis compared to the ethanol extraction with TPC content of 10.78 ± 0.94 mg GAE/g db. Phloridzin, chlorogenic acid, and quercetin were the main identified polyphenolics in the AP fractions using HPLC. DPPH radical scavenging activity of fraction B and subcritical water (SW) extracts showed comparable activity to ascorbic acid while all ethanolic extracts were cytocompatible toward human fibroblast (3T3-L1) and salivary gland acinar cells (NS-SV-AC). Our results indicated that AP is a rich source of polyphenolics with the potential for biomedical applications.https://www.mdpi.com/1420-3049/28/2/675apple pomacepolyphenolic compoundswaste valorizationantioxidant activitybiological propertiescytocompatibility |
| spellingShingle | Parinaz Hobbi Oseweuba Valentine Okoro Maryam Hajiabbas Masoud Hamidi Lei Nie Véronique Megalizzi Paul Musonge Gianina Dodi Amin Shavandi Chemical Composition, Antioxidant Activity and Cytocompatibility of Polyphenolic Compounds Extracted from Food Industry Apple Waste: Potential in Biomedical Application Molecules apple pomace polyphenolic compounds waste valorization antioxidant activity biological properties cytocompatibility |
| title | Chemical Composition, Antioxidant Activity and Cytocompatibility of Polyphenolic Compounds Extracted from Food Industry Apple Waste: Potential in Biomedical Application |
| title_full | Chemical Composition, Antioxidant Activity and Cytocompatibility of Polyphenolic Compounds Extracted from Food Industry Apple Waste: Potential in Biomedical Application |
| title_fullStr | Chemical Composition, Antioxidant Activity and Cytocompatibility of Polyphenolic Compounds Extracted from Food Industry Apple Waste: Potential in Biomedical Application |
| title_full_unstemmed | Chemical Composition, Antioxidant Activity and Cytocompatibility of Polyphenolic Compounds Extracted from Food Industry Apple Waste: Potential in Biomedical Application |
| title_short | Chemical Composition, Antioxidant Activity and Cytocompatibility of Polyphenolic Compounds Extracted from Food Industry Apple Waste: Potential in Biomedical Application |
| title_sort | chemical composition antioxidant activity and cytocompatibility of polyphenolic compounds extracted from food industry apple waste potential in biomedical application |
| topic | apple pomace polyphenolic compounds waste valorization antioxidant activity biological properties cytocompatibility |
| url | https://www.mdpi.com/1420-3049/28/2/675 |
| work_keys_str_mv | AT parinazhobbi chemicalcompositionantioxidantactivityandcytocompatibilityofpolyphenoliccompoundsextractedfromfoodindustryapplewastepotentialinbiomedicalapplication AT oseweubavalentineokoro chemicalcompositionantioxidantactivityandcytocompatibilityofpolyphenoliccompoundsextractedfromfoodindustryapplewastepotentialinbiomedicalapplication AT maryamhajiabbas chemicalcompositionantioxidantactivityandcytocompatibilityofpolyphenoliccompoundsextractedfromfoodindustryapplewastepotentialinbiomedicalapplication AT masoudhamidi chemicalcompositionantioxidantactivityandcytocompatibilityofpolyphenoliccompoundsextractedfromfoodindustryapplewastepotentialinbiomedicalapplication AT leinie chemicalcompositionantioxidantactivityandcytocompatibilityofpolyphenoliccompoundsextractedfromfoodindustryapplewastepotentialinbiomedicalapplication AT veroniquemegalizzi chemicalcompositionantioxidantactivityandcytocompatibilityofpolyphenoliccompoundsextractedfromfoodindustryapplewastepotentialinbiomedicalapplication AT paulmusonge chemicalcompositionantioxidantactivityandcytocompatibilityofpolyphenoliccompoundsextractedfromfoodindustryapplewastepotentialinbiomedicalapplication AT gianinadodi chemicalcompositionantioxidantactivityandcytocompatibilityofpolyphenoliccompoundsextractedfromfoodindustryapplewastepotentialinbiomedicalapplication AT aminshavandi chemicalcompositionantioxidantactivityandcytocompatibilityofpolyphenoliccompoundsextractedfromfoodindustryapplewastepotentialinbiomedicalapplication |