Assessing the impact of dental restorative materials on fibroblast cells: an immunohistochemical and ELISA analysis
Abstract In this study, our aim was to investigate the effects of restorative materials such as composite, compomer and high viscosity glass ionomer, which are frequently used in dentistry, on L929 fibroblast cells by evaluating the oxidative stress parameters, pro- and anti-inflammatory cytokines,...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2024-02-01
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| Series: | Scientific Reports |
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| Online Access: | https://doi.org/10.1038/s41598-024-54331-2 |
| _version_ | 1797274542200061952 |
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| author | Mustafa Duzyol Pinar Bayram Esra Duzyol Selina Aksak Karamese |
| author_facet | Mustafa Duzyol Pinar Bayram Esra Duzyol Selina Aksak Karamese |
| author_sort | Mustafa Duzyol |
| collection | DOAJ |
| description | Abstract In this study, our aim was to investigate the effects of restorative materials such as composite, compomer and high viscosity glass ionomer, which are frequently used in dentistry, on L929 fibroblast cells by evaluating the oxidative stress parameters, pro- and anti-inflammatory cytokines, and apoptosis markers. L929 fibroblast cells were cultured, and dental filling materials were applied in two doses (50 and 100 µl). Immunohistochemical staining was performed for experimental groups with Anti-Bax and Anti-Caspase 9 antibodies. Then, ELISA technique was used to detect the level of TNF-alpha, TGF-beta, IL-1-beta, IL-6, IL-10, LPO and CAT. In the light of the data, the examined dental filling materials were effective on increasing the TGF-beta, IL-10, LPO and CAT levels, and decreasing the TNF-alpha, IL-1-beta, and IL-6 levels. The histological micrographs were also support the issues. When the levels of H-score in Caspase 9 labeled micrographs were evaluated, the mean of the control group was lower than the mean of the experimental groups. Biocompatibility varies according to the content of the material, the amount of residual monomer, and its solubility. Although all the experimental groups have cytotoxic effects, the least effect is seen in the Omnichroma group. |
| first_indexed | 2024-03-07T15:00:53Z |
| format | Article |
| id | doaj.art-e41efe2bae5c4035bad5575b4f261582 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-03-07T15:00:53Z |
| publishDate | 2024-02-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-e41efe2bae5c4035bad5575b4f2615822024-03-05T19:11:40ZengNature PortfolioScientific Reports2045-23222024-02-011411810.1038/s41598-024-54331-2Assessing the impact of dental restorative materials on fibroblast cells: an immunohistochemical and ELISA analysisMustafa Duzyol0Pinar Bayram1Esra Duzyol2Selina Aksak Karamese3Department of Restorative Dentistry, Faculty of Dentistry, Istanbul Medeniyet UniversityDepartment of Histology and Embryology, Faculty of Medicine, Kafkas UniversityDepartment of Pediatric Dentistry, Faculty of Dentistry, Istanbul Medeniyet UniversityDepartment of Histology and Embryology, Faculty of Medicine, Kafkas UniversityAbstract In this study, our aim was to investigate the effects of restorative materials such as composite, compomer and high viscosity glass ionomer, which are frequently used in dentistry, on L929 fibroblast cells by evaluating the oxidative stress parameters, pro- and anti-inflammatory cytokines, and apoptosis markers. L929 fibroblast cells were cultured, and dental filling materials were applied in two doses (50 and 100 µl). Immunohistochemical staining was performed for experimental groups with Anti-Bax and Anti-Caspase 9 antibodies. Then, ELISA technique was used to detect the level of TNF-alpha, TGF-beta, IL-1-beta, IL-6, IL-10, LPO and CAT. In the light of the data, the examined dental filling materials were effective on increasing the TGF-beta, IL-10, LPO and CAT levels, and decreasing the TNF-alpha, IL-1-beta, and IL-6 levels. The histological micrographs were also support the issues. When the levels of H-score in Caspase 9 labeled micrographs were evaluated, the mean of the control group was lower than the mean of the experimental groups. Biocompatibility varies according to the content of the material, the amount of residual monomer, and its solubility. Although all the experimental groups have cytotoxic effects, the least effect is seen in the Omnichroma group.https://doi.org/10.1038/s41598-024-54331-2FibroblastDental filling materialsInflammationOxidative stressApoptosis |
| spellingShingle | Mustafa Duzyol Pinar Bayram Esra Duzyol Selina Aksak Karamese Assessing the impact of dental restorative materials on fibroblast cells: an immunohistochemical and ELISA analysis Scientific Reports Fibroblast Dental filling materials Inflammation Oxidative stress Apoptosis |
| title | Assessing the impact of dental restorative materials on fibroblast cells: an immunohistochemical and ELISA analysis |
| title_full | Assessing the impact of dental restorative materials on fibroblast cells: an immunohistochemical and ELISA analysis |
| title_fullStr | Assessing the impact of dental restorative materials on fibroblast cells: an immunohistochemical and ELISA analysis |
| title_full_unstemmed | Assessing the impact of dental restorative materials on fibroblast cells: an immunohistochemical and ELISA analysis |
| title_short | Assessing the impact of dental restorative materials on fibroblast cells: an immunohistochemical and ELISA analysis |
| title_sort | assessing the impact of dental restorative materials on fibroblast cells an immunohistochemical and elisa analysis |
| topic | Fibroblast Dental filling materials Inflammation Oxidative stress Apoptosis |
| url | https://doi.org/10.1038/s41598-024-54331-2 |
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