Considerations for colorblind individuals on selecting colorimetric or fluorescent dye assay outcomes
A disadvantage of colorimetric detection in nucleic acid amplification assays is the possibility that a colorblind individual may interpret colors differently than observers with full-color vision. Using an isothermal amplification assay, the ability of colorblind individuals to distinguish between...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Future Science Ltd
2023-12-01
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| Series: | BioTechniques |
| Subjects: | |
| Online Access: | https://www.future-science.com/doi/10.2144/btn-2023-0041 |
| _version_ | 1797386273194770432 |
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| author | Kirstyn Loyva Erik Hofmeister Fiona Georgousi Constance Roderick Rebecca Cole |
| author_facet | Kirstyn Loyva Erik Hofmeister Fiona Georgousi Constance Roderick Rebecca Cole |
| author_sort | Kirstyn Loyva |
| collection | DOAJ |
| description | A disadvantage of colorimetric detection in nucleic acid amplification assays is the possibility that a colorblind individual may interpret colors differently than observers with full-color vision. Using an isothermal amplification assay, the ability of colorblind individuals to distinguish between positive and negative results for four dyes was tested. Five individuals with self-reported colorblindness and four with full-color vision reported their observations of the color of the solution. Although colorblind individuals may accurately interpret assay results, they were often not accurate in reporting the color. Hydroxynaphthol blue was the most problematic dye, and both phenol red and SYBR™ green were less troublesome. Consideration for colorblind individuals is warranted when developing an assay and training staff in its performance. |
| first_indexed | 2024-03-08T22:07:56Z |
| format | Article |
| id | doaj.art-e421a9bc364f4fee9d06c1ccc5371f16 |
| institution | Directory Open Access Journal |
| issn | 0736-6205 1940-9818 |
| language | English |
| last_indexed | 2024-03-08T22:07:56Z |
| publishDate | 2023-12-01 |
| publisher | Future Science Ltd |
| record_format | Article |
| series | BioTechniques |
| spelling | doaj.art-e421a9bc364f4fee9d06c1ccc5371f162023-12-19T08:16:44ZengFuture Science LtdBioTechniques0736-62051940-98182023-12-0175624024410.2144/btn-2023-0041Considerations for colorblind individuals on selecting colorimetric or fluorescent dye assay outcomesKirstyn Loyva0Erik Hofmeister1Fiona Georgousi2Constance Roderick3Rebecca Cole41U.S. Geological Survey, National Wildlife Health Center, Madison, WI 53711, USA1U.S. Geological Survey, National Wildlife Health Center, Madison, WI 53711, USA1U.S. Geological Survey, National Wildlife Health Center, Madison, WI 53711, USA1U.S. Geological Survey, National Wildlife Health Center, Madison, WI 53711, USA1U.S. Geological Survey, National Wildlife Health Center, Madison, WI 53711, USAA disadvantage of colorimetric detection in nucleic acid amplification assays is the possibility that a colorblind individual may interpret colors differently than observers with full-color vision. Using an isothermal amplification assay, the ability of colorblind individuals to distinguish between positive and negative results for four dyes was tested. Five individuals with self-reported colorblindness and four with full-color vision reported their observations of the color of the solution. Although colorblind individuals may accurately interpret assay results, they were often not accurate in reporting the color. Hydroxynaphthol blue was the most problematic dye, and both phenol red and SYBR™ green were less troublesome. Consideration for colorblind individuals is warranted when developing an assay and training staff in its performance.https://www.future-science.com/doi/10.2144/btn-2023-0041color blindnesscolorimetric DNA detectionfluorescent DNA detectionisothermal amplificationLAMP amplification |
| spellingShingle | Kirstyn Loyva Erik Hofmeister Fiona Georgousi Constance Roderick Rebecca Cole Considerations for colorblind individuals on selecting colorimetric or fluorescent dye assay outcomes BioTechniques color blindness colorimetric DNA detection fluorescent DNA detection isothermal amplification LAMP amplification |
| title | Considerations for colorblind individuals on selecting colorimetric or fluorescent dye assay outcomes |
| title_full | Considerations for colorblind individuals on selecting colorimetric or fluorescent dye assay outcomes |
| title_fullStr | Considerations for colorblind individuals on selecting colorimetric or fluorescent dye assay outcomes |
| title_full_unstemmed | Considerations for colorblind individuals on selecting colorimetric or fluorescent dye assay outcomes |
| title_short | Considerations for colorblind individuals on selecting colorimetric or fluorescent dye assay outcomes |
| title_sort | considerations for colorblind individuals on selecting colorimetric or fluorescent dye assay outcomes |
| topic | color blindness colorimetric DNA detection fluorescent DNA detection isothermal amplification LAMP amplification |
| url | https://www.future-science.com/doi/10.2144/btn-2023-0041 |
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