Thermo-Sensitive Poly (N-isopropylacrylamide-co-polyacrylamide) Hydrogel for pH-Responsive Therapeutic Delivery
Stimuli-response polymeric nanoparticles have emerged as a carrier system for various types of therapeutic delivery. In this study, we prepared a dual pH- and thermo-sensitive copolymer hydrogel (HG) system (PNIPAm-co-PAAm HG), using N-isopropyl acrylamide (NIPAm) and acrylamide (AAm) as comonomers....
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| Format: | Article |
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MDPI AG
2022-10-01
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| Series: | Polymers |
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| Online Access: | https://www.mdpi.com/2073-4360/14/19/4128 |
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| author | Madhappan Santhamoorthy Thi Tuong Vy Phan Vanaraj Ramkumar Chaitany Jayprakash Raorane Kokila Thirupathi Seong-Cheol Kim |
| author_facet | Madhappan Santhamoorthy Thi Tuong Vy Phan Vanaraj Ramkumar Chaitany Jayprakash Raorane Kokila Thirupathi Seong-Cheol Kim |
| author_sort | Madhappan Santhamoorthy |
| collection | DOAJ |
| description | Stimuli-response polymeric nanoparticles have emerged as a carrier system for various types of therapeutic delivery. In this study, we prepared a dual pH- and thermo-sensitive copolymer hydrogel (HG) system (PNIPAm-co-PAAm HG), using N-isopropyl acrylamide (NIPAm) and acrylamide (AAm) as comonomers. The synthesized PNIPAm-co-PAAm HG was characterized using various instrumental characterizations. Moreover, the PNIPAm-co-PAAm HG’s thermoresponsive phase transition behavior was investigated, and the results showed that the prepared HG responds to temperature changes. In vitro drug loading and release behavior of PNIPAm-co-PAAm HG was investigated using Curcumin (Cur) as the model cargo under different pH and temperature conditions. The PNIPAm-co-PAAm HG showed pH and temperature-responsive drug release behavior and demonstrated about 65% Cur loading efficiency. A nearly complete release of the loaded Cur occurred from the PNIPAm-co-PAAm HG over 4 h at pH 5.5 and 40 °C. The cytotoxicity study was performed on a liver cancer cell line (HepG2 cells), which revealed that the prepared PNIPAm-co-PAAm HG showed good biocompatibility, suggesting that it could be applied as a drug delivery carrier. Moreover, the in vitro cytocompatibility test (MTT assay) results revealed that the PNIPAm-co-PAAm HG is biocompatible. Therefore, the PNIPAm-co-PAAm HG has the potential to be useful in the delivery of drugs in solid tumor-targeted therapy. |
| first_indexed | 2024-03-09T21:16:24Z |
| format | Article |
| id | doaj.art-e4231eb0ea6b4808b5a3312c2905693b |
| institution | Directory Open Access Journal |
| issn | 2073-4360 |
| language | English |
| last_indexed | 2024-03-09T21:16:24Z |
| publishDate | 2022-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Polymers |
| spelling | doaj.art-e4231eb0ea6b4808b5a3312c2905693b2023-11-23T21:34:46ZengMDPI AGPolymers2073-43602022-10-011419412810.3390/polym14194128Thermo-Sensitive Poly (N-isopropylacrylamide-co-polyacrylamide) Hydrogel for pH-Responsive Therapeutic DeliveryMadhappan Santhamoorthy0Thi Tuong Vy Phan1Vanaraj Ramkumar2Chaitany Jayprakash Raorane3Kokila Thirupathi4Seong-Cheol Kim5School of Chemical Engineering, Yeungnam University, Gyeongsan 38541, KoreaCenter for Advanced Chemistry, Institute of Research and Development, Duy Tan University, 03 Quang Trung, Hai Chau, Danang 550000, VietnamSchool of Chemical Engineering, Yeungnam University, Gyeongsan 38541, KoreaSchool of Chemical Engineering, Yeungnam University, Gyeongsan 38541, KoreaDepartment of Physics, Sri Moogambigai College of Arts and Science for Women, Palacode 636808, IndiaSchool of Chemical Engineering, Yeungnam University, Gyeongsan 38541, KoreaStimuli-response polymeric nanoparticles have emerged as a carrier system for various types of therapeutic delivery. In this study, we prepared a dual pH- and thermo-sensitive copolymer hydrogel (HG) system (PNIPAm-co-PAAm HG), using N-isopropyl acrylamide (NIPAm) and acrylamide (AAm) as comonomers. The synthesized PNIPAm-co-PAAm HG was characterized using various instrumental characterizations. Moreover, the PNIPAm-co-PAAm HG’s thermoresponsive phase transition behavior was investigated, and the results showed that the prepared HG responds to temperature changes. In vitro drug loading and release behavior of PNIPAm-co-PAAm HG was investigated using Curcumin (Cur) as the model cargo under different pH and temperature conditions. The PNIPAm-co-PAAm HG showed pH and temperature-responsive drug release behavior and demonstrated about 65% Cur loading efficiency. A nearly complete release of the loaded Cur occurred from the PNIPAm-co-PAAm HG over 4 h at pH 5.5 and 40 °C. The cytotoxicity study was performed on a liver cancer cell line (HepG2 cells), which revealed that the prepared PNIPAm-co-PAAm HG showed good biocompatibility, suggesting that it could be applied as a drug delivery carrier. Moreover, the in vitro cytocompatibility test (MTT assay) results revealed that the PNIPAm-co-PAAm HG is biocompatible. Therefore, the PNIPAm-co-PAAm HG has the potential to be useful in the delivery of drugs in solid tumor-targeted therapy.https://www.mdpi.com/2073-4360/14/19/4128copolymerNIPAmacrylamidecurcumin deliverypH and temperature stimulicancer therapy |
| spellingShingle | Madhappan Santhamoorthy Thi Tuong Vy Phan Vanaraj Ramkumar Chaitany Jayprakash Raorane Kokila Thirupathi Seong-Cheol Kim Thermo-Sensitive Poly (N-isopropylacrylamide-co-polyacrylamide) Hydrogel for pH-Responsive Therapeutic Delivery Polymers copolymer NIPAm acrylamide curcumin delivery pH and temperature stimuli cancer therapy |
| title | Thermo-Sensitive Poly (N-isopropylacrylamide-co-polyacrylamide) Hydrogel for pH-Responsive Therapeutic Delivery |
| title_full | Thermo-Sensitive Poly (N-isopropylacrylamide-co-polyacrylamide) Hydrogel for pH-Responsive Therapeutic Delivery |
| title_fullStr | Thermo-Sensitive Poly (N-isopropylacrylamide-co-polyacrylamide) Hydrogel for pH-Responsive Therapeutic Delivery |
| title_full_unstemmed | Thermo-Sensitive Poly (N-isopropylacrylamide-co-polyacrylamide) Hydrogel for pH-Responsive Therapeutic Delivery |
| title_short | Thermo-Sensitive Poly (N-isopropylacrylamide-co-polyacrylamide) Hydrogel for pH-Responsive Therapeutic Delivery |
| title_sort | thermo sensitive poly n isopropylacrylamide co polyacrylamide hydrogel for ph responsive therapeutic delivery |
| topic | copolymer NIPAm acrylamide curcumin delivery pH and temperature stimuli cancer therapy |
| url | https://www.mdpi.com/2073-4360/14/19/4128 |
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