Evaluation of a matrix-assisted laser desorption ionization-time of flight mass spectrometry assisted, selective broth method to screen for vancomycin-resistant enterococci in patients at high risk.
<h4>Background</h4>Bile esculin azide with vancomycin (BEAV) medium is a sensitive, but slightly less specific method for vancomycin-resistant enterococci (VRE) screening. Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) is a rapid method for id...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2017-01-01
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| Series: | PLoS ONE |
| Online Access: | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0179455&type=printable |
| _version_ | 1826579463584874496 |
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| author | Tsi-Shu Huang Susan Shin-Jung Lee Chia-Chien Lee Chiu-Yen Chen Fang-Chen Chen Bao-Chen Chen Cheng Len Sy Kuan-Sheng Wu |
| author_facet | Tsi-Shu Huang Susan Shin-Jung Lee Chia-Chien Lee Chiu-Yen Chen Fang-Chen Chen Bao-Chen Chen Cheng Len Sy Kuan-Sheng Wu |
| author_sort | Tsi-Shu Huang |
| collection | DOAJ |
| description | <h4>Background</h4>Bile esculin azide with vancomycin (BEAV) medium is a sensitive, but slightly less specific method for vancomycin-resistant enterococci (VRE) screening. Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) is a rapid method for identification of clinical pathogens. This study aimed to assess the performance of a novel combination screening test for VRE, using BEAV broth combined with MALDI-TOF MS.<h4>Materials and methods</h4>Clinical specimens were collected from patients at risk of VRE carriage, and tested by the novel combination method, using selective BEAV broth culture method followed by MALDI-TOF MS identification (SBEAVM). The reference method used for comparison was the ChromID VRE agar method.<h4>Results</h4>A total of 135 specimens were collected from 78 patients, and 63 specimens tested positive for VRE positive using the ChromID VRE method (positive rate 46.7%). The sensitivity, specificity, positive predictive value, and negative predictive value of SBEAVM method after an incubation period of 28 hours were 93.7%, 90.3%, 89.4%, and 94.2%, respectively. The SBEAVM method when compared to the ChromID VRE method had a shorter turnaround time (29 vs. 48-72 hours) and lower laboratory cost ($2.11 vs. $3.23 per test).<h4>Conclusions</h4>This study demonstrates that SBEAVM is a rapid, inexpensive, and accurate method for use in VRE screening. |
| first_indexed | 2024-04-13T13:13:30Z |
| format | Article |
| id | doaj.art-e423d1d4b61a46188b52f714d335bc60 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2025-03-14T14:18:41Z |
| publishDate | 2017-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-e423d1d4b61a46188b52f714d335bc602025-02-27T05:32:51ZengPublic Library of Science (PLoS)PLoS ONE1932-62032017-01-01126e017945510.1371/journal.pone.0179455Evaluation of a matrix-assisted laser desorption ionization-time of flight mass spectrometry assisted, selective broth method to screen for vancomycin-resistant enterococci in patients at high risk.Tsi-Shu HuangSusan Shin-Jung LeeChia-Chien LeeChiu-Yen ChenFang-Chen ChenBao-Chen ChenCheng Len SyKuan-Sheng Wu<h4>Background</h4>Bile esculin azide with vancomycin (BEAV) medium is a sensitive, but slightly less specific method for vancomycin-resistant enterococci (VRE) screening. Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) is a rapid method for identification of clinical pathogens. This study aimed to assess the performance of a novel combination screening test for VRE, using BEAV broth combined with MALDI-TOF MS.<h4>Materials and methods</h4>Clinical specimens were collected from patients at risk of VRE carriage, and tested by the novel combination method, using selective BEAV broth culture method followed by MALDI-TOF MS identification (SBEAVM). The reference method used for comparison was the ChromID VRE agar method.<h4>Results</h4>A total of 135 specimens were collected from 78 patients, and 63 specimens tested positive for VRE positive using the ChromID VRE method (positive rate 46.7%). The sensitivity, specificity, positive predictive value, and negative predictive value of SBEAVM method after an incubation period of 28 hours were 93.7%, 90.3%, 89.4%, and 94.2%, respectively. The SBEAVM method when compared to the ChromID VRE method had a shorter turnaround time (29 vs. 48-72 hours) and lower laboratory cost ($2.11 vs. $3.23 per test).<h4>Conclusions</h4>This study demonstrates that SBEAVM is a rapid, inexpensive, and accurate method for use in VRE screening.https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0179455&type=printable |
| spellingShingle | Tsi-Shu Huang Susan Shin-Jung Lee Chia-Chien Lee Chiu-Yen Chen Fang-Chen Chen Bao-Chen Chen Cheng Len Sy Kuan-Sheng Wu Evaluation of a matrix-assisted laser desorption ionization-time of flight mass spectrometry assisted, selective broth method to screen for vancomycin-resistant enterococci in patients at high risk. PLoS ONE |
| title | Evaluation of a matrix-assisted laser desorption ionization-time of flight mass spectrometry assisted, selective broth method to screen for vancomycin-resistant enterococci in patients at high risk. |
| title_full | Evaluation of a matrix-assisted laser desorption ionization-time of flight mass spectrometry assisted, selective broth method to screen for vancomycin-resistant enterococci in patients at high risk. |
| title_fullStr | Evaluation of a matrix-assisted laser desorption ionization-time of flight mass spectrometry assisted, selective broth method to screen for vancomycin-resistant enterococci in patients at high risk. |
| title_full_unstemmed | Evaluation of a matrix-assisted laser desorption ionization-time of flight mass spectrometry assisted, selective broth method to screen for vancomycin-resistant enterococci in patients at high risk. |
| title_short | Evaluation of a matrix-assisted laser desorption ionization-time of flight mass spectrometry assisted, selective broth method to screen for vancomycin-resistant enterococci in patients at high risk. |
| title_sort | evaluation of a matrix assisted laser desorption ionization time of flight mass spectrometry assisted selective broth method to screen for vancomycin resistant enterococci in patients at high risk |
| url | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0179455&type=printable |
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