Successful production of offspring derived from mouse zygotes vitrified with carboxylated ε-poly-L-lysine and polyvinyl alcohol without serum
The vitrification of zygotes is important for their use as donors for generating genome-edited mice. We previously reported the successful vitrification of mouse zygotes using carboxylated ε-poly-L-lysine (COOH-PLL). However, this vitrification solution contains fetal calf serum (FCS), which contain...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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The Society for Reproduction and Development
2022-12-01
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| Series: | The Journal of Reproduction and Development |
| Subjects: | |
| Online Access: | https://www.jstage.jst.go.jp/article/jrd/69/1/69_2022-121/_pdf/-char/en |
| _version_ | 1797633967166324736 |
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| author | Midori ISHII Maki KAMOSHITA Yoshihiro KURIHARA Kazuaki MATSUMURA Suong-Hyu HYON Junya ITO Naomi KASHIWAZAKI |
| author_facet | Midori ISHII Maki KAMOSHITA Yoshihiro KURIHARA Kazuaki MATSUMURA Suong-Hyu HYON Junya ITO Naomi KASHIWAZAKI |
| author_sort | Midori ISHII |
| collection | DOAJ |
| description | The vitrification of zygotes is important for their use as donors for generating genome-edited mice. We previously reported the successful vitrification of mouse zygotes using carboxylated ε-poly-L-lysine (COOH-PLL). However, this vitrification solution contains fetal calf serum (FCS), which contains unknown factors and presents risks of pathogenic viral and microbial contamination. In this study, we examined whether polyvinyl alcohol (PVA) can be used as an alternative to FCS in vitrification solutions for mouse zygotes. When COOH-PLL was added to the vitrification solutions, zygotes vitrified with solutions containing 0.01% PVA (PV0.01) and those vitrified in a control solution containing FCS (75.6%) developed into blastocysts (78.4%). In addition, there were no significant differences in the ability to develop to term between the control solution (46.6%) and PV0.01 (44.1%) groups. In conclusion, we clearly demonstrated that PVA can replace FCS in our vitrification solution supplemented with COOH-PLL for mouse zygotes. |
| first_indexed | 2024-03-11T12:02:08Z |
| format | Article |
| id | doaj.art-e425303e091340d59f3cecb20ef25df4 |
| institution | Directory Open Access Journal |
| issn | 0916-8818 1348-4400 |
| language | English |
| last_indexed | 2024-03-11T12:02:08Z |
| publishDate | 2022-12-01 |
| publisher | The Society for Reproduction and Development |
| record_format | Article |
| series | The Journal of Reproduction and Development |
| spelling | doaj.art-e425303e091340d59f3cecb20ef25df42023-11-08T04:44:07ZengThe Society for Reproduction and DevelopmentThe Journal of Reproduction and Development0916-88181348-44002022-12-01691535510.1262/jrd.2022-121jrdSuccessful production of offspring derived from mouse zygotes vitrified with carboxylated ε-poly-L-lysine and polyvinyl alcohol without serumMidori ISHII0Maki KAMOSHITA1Yoshihiro KURIHARA2Kazuaki MATSUMURA3Suong-Hyu HYON4Junya ITO5Naomi KASHIWAZAKI6School of Veterinary Medicine, Azabu University, Sagamihara 252-5201, JapanGraduate School of Veterinary Sciences, Azabu University, Sagamihara 252-5201, JapanSchool of Veterinary Medicine, Azabu University, Sagamihara 252-5201, JapanSchool of Materials Science, Japan Advanced Institute of Science and Technology, Nomi 923-1291, JapanBMG Incorporated, Higashikujo 601-8023, JapanSchool of Veterinary Medicine, Azabu University, Sagamihara 252-5201, JapanSchool of Veterinary Medicine, Azabu University, Sagamihara 252-5201, JapanThe vitrification of zygotes is important for their use as donors for generating genome-edited mice. We previously reported the successful vitrification of mouse zygotes using carboxylated ε-poly-L-lysine (COOH-PLL). However, this vitrification solution contains fetal calf serum (FCS), which contains unknown factors and presents risks of pathogenic viral and microbial contamination. In this study, we examined whether polyvinyl alcohol (PVA) can be used as an alternative to FCS in vitrification solutions for mouse zygotes. When COOH-PLL was added to the vitrification solutions, zygotes vitrified with solutions containing 0.01% PVA (PV0.01) and those vitrified in a control solution containing FCS (75.6%) developed into blastocysts (78.4%). In addition, there were no significant differences in the ability to develop to term between the control solution (46.6%) and PV0.01 (44.1%) groups. In conclusion, we clearly demonstrated that PVA can replace FCS in our vitrification solution supplemented with COOH-PLL for mouse zygotes.https://www.jstage.jst.go.jp/article/jrd/69/1/69_2022-121/_pdf/-char/encarboxylated ε-poly-l-lysinecooh-pllmousevitrificationzygote |
| spellingShingle | Midori ISHII Maki KAMOSHITA Yoshihiro KURIHARA Kazuaki MATSUMURA Suong-Hyu HYON Junya ITO Naomi KASHIWAZAKI Successful production of offspring derived from mouse zygotes vitrified with carboxylated ε-poly-L-lysine and polyvinyl alcohol without serum The Journal of Reproduction and Development carboxylated ε-poly-l-lysine cooh-pll mouse vitrification zygote |
| title | Successful production of offspring derived from mouse zygotes vitrified with carboxylated ε-poly-L-lysine and polyvinyl alcohol without serum |
| title_full | Successful production of offspring derived from mouse zygotes vitrified with carboxylated ε-poly-L-lysine and polyvinyl alcohol without serum |
| title_fullStr | Successful production of offspring derived from mouse zygotes vitrified with carboxylated ε-poly-L-lysine and polyvinyl alcohol without serum |
| title_full_unstemmed | Successful production of offspring derived from mouse zygotes vitrified with carboxylated ε-poly-L-lysine and polyvinyl alcohol without serum |
| title_short | Successful production of offspring derived from mouse zygotes vitrified with carboxylated ε-poly-L-lysine and polyvinyl alcohol without serum |
| title_sort | successful production of offspring derived from mouse zygotes vitrified with carboxylated ε poly l lysine and polyvinyl alcohol without serum |
| topic | carboxylated ε-poly-l-lysine cooh-pll mouse vitrification zygote |
| url | https://www.jstage.jst.go.jp/article/jrd/69/1/69_2022-121/_pdf/-char/en |
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