Biological control for basal rot in yellow pitahaya fruits (Selenicereus megalanthus): Ex vivo trials
Endophytic microbiome for biocontrol of plant pathogens has been the subject of recent research. Fusarium oxysporum (F54) and Fusarium fujikuroi (F17) are fungi associated with basal rot in yellow pitahaya fruits (Selenicereus megalanthus), which limits the possibilities of fruit commercialization....
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| Format: | Article |
| Language: | English |
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Elsevier
2022-07-01
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| Series: | Journal of King Saud University: Science |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1018364722002233 |
| _version_ | 1811342027688771584 |
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| author | Luisa F. Lozada Cristobal N. Aguilar Claudia L. Vargas Carolina M. Bedoya Liliana Serna-Cock |
| author_facet | Luisa F. Lozada Cristobal N. Aguilar Claudia L. Vargas Carolina M. Bedoya Liliana Serna-Cock |
| author_sort | Luisa F. Lozada |
| collection | DOAJ |
| description | Endophytic microbiome for biocontrol of plant pathogens has been the subject of recent research. Fusarium oxysporum (F54) and Fusarium fujikuroi (F17) are fungi associated with basal rot in yellow pitahaya fruits (Selenicereus megalanthus), which limits the possibilities of fruit commercialization. Objective: In this study, the ability of Lactobacillus plantarum (endophytic bacterium), Weissella cibaria (non-endophytic), and their metabolites to control the basal rot of yellow pitahaya (caused by fungi F54 and F17) was evaluated. Methods: Healthy yellow pitahaya fruit was sprayed with lactic acid bacteria or their metabolites, and one hour later, spore solutions of F54 and F17 were individually injected into the fruit peduncle. The affected area of the peduncle was measured at 15 days. The percentage of the area of infection was measured using image evaluation software. The percentage reduction of infection was also calculated. Results: When healthy fruits were inoculated with pathogens (positive control), these developed a severe disease (more than 14 cm2 of infection). Healthy fruits sprayed with W. cibaria and L. plantarum or their metabolites (negative control without pathogens) remained healthy for more than 15 days. Treatments without lactic acid bacteria began with symptoms of senescence on day 9. L. plantarum (and its metabolites) reduced the manifestations of basal rot in yellow pitahaya fruits by up to 99.99%. Conclusions: Specific microbiome of yellow pitahaya as L. plantarum is an effective biological control agent against Fusarium associated with basal rot. L. plantarum and its metabolites could reduce the environmental impact caused by synthetic fungicides used to control basal rot of yellow pitahaya. Finally, the results of this research suggest that a fungal consortium may not be necessary to develop the basal rot disease. |
| first_indexed | 2024-04-13T19:04:09Z |
| format | Article |
| id | doaj.art-e425688f47ae4758969f02cdc6db8add |
| institution | Directory Open Access Journal |
| issn | 1018-3647 |
| language | English |
| last_indexed | 2024-04-13T19:04:09Z |
| publishDate | 2022-07-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of King Saud University: Science |
| spelling | doaj.art-e425688f47ae4758969f02cdc6db8add2022-12-22T02:34:01ZengElsevierJournal of King Saud University: Science1018-36472022-07-01345102042Biological control for basal rot in yellow pitahaya fruits (Selenicereus megalanthus): Ex vivo trialsLuisa F. Lozada0Cristobal N. Aguilar1Claudia L. Vargas2Carolina M. Bedoya3Liliana Serna-Cock4Lactic Acid Bacteria Research Group, Faculty of Engineering and Administration, Universidad Nacional de Colombia Campus Palmira, Palmira, Valle del Cauca, ColombiaBioprocesses and Bioproducts Research Group, Food Research Department, School of Chemistry, Universidad Autonoma de Coahuila, Coahuila, Mexico; Corresponding author.Universidad del Valle, Faculty of Engineering Colombia, Cali, Valle del Cauca, ColombiaUniversidad Nacional Abierta y a Distancia, Dosquebradas, ColombiaLactic Acid Bacteria Research Group, Faculty of Engineering and Administration, Universidad Nacional de Colombia Campus Palmira, Palmira, Valle del Cauca, ColombiaEndophytic microbiome for biocontrol of plant pathogens has been the subject of recent research. Fusarium oxysporum (F54) and Fusarium fujikuroi (F17) are fungi associated with basal rot in yellow pitahaya fruits (Selenicereus megalanthus), which limits the possibilities of fruit commercialization. Objective: In this study, the ability of Lactobacillus plantarum (endophytic bacterium), Weissella cibaria (non-endophytic), and their metabolites to control the basal rot of yellow pitahaya (caused by fungi F54 and F17) was evaluated. Methods: Healthy yellow pitahaya fruit was sprayed with lactic acid bacteria or their metabolites, and one hour later, spore solutions of F54 and F17 were individually injected into the fruit peduncle. The affected area of the peduncle was measured at 15 days. The percentage of the area of infection was measured using image evaluation software. The percentage reduction of infection was also calculated. Results: When healthy fruits were inoculated with pathogens (positive control), these developed a severe disease (more than 14 cm2 of infection). Healthy fruits sprayed with W. cibaria and L. plantarum or their metabolites (negative control without pathogens) remained healthy for more than 15 days. Treatments without lactic acid bacteria began with symptoms of senescence on day 9. L. plantarum (and its metabolites) reduced the manifestations of basal rot in yellow pitahaya fruits by up to 99.99%. Conclusions: Specific microbiome of yellow pitahaya as L. plantarum is an effective biological control agent against Fusarium associated with basal rot. L. plantarum and its metabolites could reduce the environmental impact caused by synthetic fungicides used to control basal rot of yellow pitahaya. Finally, the results of this research suggest that a fungal consortium may not be necessary to develop the basal rot disease.http://www.sciencedirect.com/science/article/pii/S1018364722002233Lactic acid bacteriaMicrobiomeFusarium oxysporumWeissella cibaria |
| spellingShingle | Luisa F. Lozada Cristobal N. Aguilar Claudia L. Vargas Carolina M. Bedoya Liliana Serna-Cock Biological control for basal rot in yellow pitahaya fruits (Selenicereus megalanthus): Ex vivo trials Journal of King Saud University: Science Lactic acid bacteria Microbiome Fusarium oxysporum Weissella cibaria |
| title | Biological control for basal rot in yellow pitahaya fruits (Selenicereus megalanthus): Ex vivo trials |
| title_full | Biological control for basal rot in yellow pitahaya fruits (Selenicereus megalanthus): Ex vivo trials |
| title_fullStr | Biological control for basal rot in yellow pitahaya fruits (Selenicereus megalanthus): Ex vivo trials |
| title_full_unstemmed | Biological control for basal rot in yellow pitahaya fruits (Selenicereus megalanthus): Ex vivo trials |
| title_short | Biological control for basal rot in yellow pitahaya fruits (Selenicereus megalanthus): Ex vivo trials |
| title_sort | biological control for basal rot in yellow pitahaya fruits selenicereus megalanthus ex vivo trials |
| topic | Lactic acid bacteria Microbiome Fusarium oxysporum Weissella cibaria |
| url | http://www.sciencedirect.com/science/article/pii/S1018364722002233 |
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