Christensenella minuta interacts with multiple gut bacteria
IntroductionGut microbes form complex networks that significantly influence host health and disease treatment. Interventions with the probiotic bacteria on the gut microbiota have been demonstrated to improve host well-being. As a representative of next-generation probiotics, Christensenella minuta...
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| Format: | Article |
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Frontiers Media S.A.
2024-02-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2024.1301073/full |
| _version_ | 1797303420238954496 |
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| author | Chang Xu He Jiang Li-Juan Feng Min-Zhi Jiang Yu-Lin Wang Shuang-Jiang Liu Shuang-Jiang Liu |
| author_facet | Chang Xu He Jiang Li-Juan Feng Min-Zhi Jiang Yu-Lin Wang Shuang-Jiang Liu Shuang-Jiang Liu |
| author_sort | Chang Xu |
| collection | DOAJ |
| description | IntroductionGut microbes form complex networks that significantly influence host health and disease treatment. Interventions with the probiotic bacteria on the gut microbiota have been demonstrated to improve host well-being. As a representative of next-generation probiotics, Christensenella minuta (C. minuta) plays a critical role in regulating energy balance and metabolic homeostasis in human bodies, showing potential in treating metabolic disorders and reducing inflammation. However, interactions of C. minuta with the members of the networked gut microbiota have rarely been explored.MethodsIn this study, we investigated the impact of C. minuta on fecal microbiota via metagenomic sequencing, focusing on retrieving bacterial strains and coculture assays of C. minuta with associated microbial partners.ResultsOur results showed that C. minuta intervention significantly reduced the diversity of fecal microorganisms, but specifically enhanced some groups of bacteria, such as Lactobacillaceae. C. minuta selectively enriched bacterial pathways that compensated for its metabolic defects on vitamin B1, B12, serine, and glutamate synthesis. Meanwhile, C. minuta cross-feeds Faecalibacterium prausnitzii and other bacteria via the production of arginine, branched-chain amino acids, fumaric acids and short-chain fatty acids (SCFAs), such as acetic. Both metagenomic data analysis and culture experiments revealed that C. minuta negatively correlated with Klebsiella pneumoniae and 14 other bacterial taxa, while positively correlated with F. prausnitzii. Our results advance our comprehension of C. minuta’s in modulating the gut microbial network.ConclusionsC. minuta disrupts the composition of the fecal microbiota. This disturbance is manifested through cross-feeding, nutritional competition, and supplementation of its own metabolic deficiencies, resulting in the specific enrichment or inhibition of the growth of certain bacteria. This study will shed light on the application of C. minuta as a probiotic for effective interventions on gut microbiomes and improvement of host health. |
| first_indexed | 2024-03-07T23:52:30Z |
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| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-03-07T23:52:30Z |
| publishDate | 2024-02-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-e426bcb327f842f6ba630ec6564f1ac12024-02-19T04:36:59ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2024-02-011510.3389/fmicb.2024.13010731301073Christensenella minuta interacts with multiple gut bacteriaChang Xu0He Jiang1Li-Juan Feng2Min-Zhi Jiang3Yu-Lin Wang4Shuang-Jiang Liu5Shuang-Jiang Liu6State Key Laboratory of Microbial Technology, Shandong University, Qingdao, ChinaState Key Laboratory of Microbial Technology, Shandong University, Qingdao, ChinaState Key Laboratory of Microbial Technology, Shandong University, Qingdao, ChinaState Key Laboratory of Microbial Technology, Shandong University, Qingdao, ChinaState Key Laboratory of Microbial Technology, Shandong University, Qingdao, ChinaState Key Laboratory of Microbial Technology, Shandong University, Qingdao, ChinaState Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing, ChinaIntroductionGut microbes form complex networks that significantly influence host health and disease treatment. Interventions with the probiotic bacteria on the gut microbiota have been demonstrated to improve host well-being. As a representative of next-generation probiotics, Christensenella minuta (C. minuta) plays a critical role in regulating energy balance and metabolic homeostasis in human bodies, showing potential in treating metabolic disorders and reducing inflammation. However, interactions of C. minuta with the members of the networked gut microbiota have rarely been explored.MethodsIn this study, we investigated the impact of C. minuta on fecal microbiota via metagenomic sequencing, focusing on retrieving bacterial strains and coculture assays of C. minuta with associated microbial partners.ResultsOur results showed that C. minuta intervention significantly reduced the diversity of fecal microorganisms, but specifically enhanced some groups of bacteria, such as Lactobacillaceae. C. minuta selectively enriched bacterial pathways that compensated for its metabolic defects on vitamin B1, B12, serine, and glutamate synthesis. Meanwhile, C. minuta cross-feeds Faecalibacterium prausnitzii and other bacteria via the production of arginine, branched-chain amino acids, fumaric acids and short-chain fatty acids (SCFAs), such as acetic. Both metagenomic data analysis and culture experiments revealed that C. minuta negatively correlated with Klebsiella pneumoniae and 14 other bacterial taxa, while positively correlated with F. prausnitzii. Our results advance our comprehension of C. minuta’s in modulating the gut microbial network.ConclusionsC. minuta disrupts the composition of the fecal microbiota. This disturbance is manifested through cross-feeding, nutritional competition, and supplementation of its own metabolic deficiencies, resulting in the specific enrichment or inhibition of the growth of certain bacteria. This study will shed light on the application of C. minuta as a probiotic for effective interventions on gut microbiomes and improvement of host health.https://www.frontiersin.org/articles/10.3389/fmicb.2024.1301073/fullChristensenellaceaeintestinal microorganismco-occurrence networkFaecalibacterium prausnitziiKlebsiella pneumoniaenutrient cross-feeding |
| spellingShingle | Chang Xu He Jiang Li-Juan Feng Min-Zhi Jiang Yu-Lin Wang Shuang-Jiang Liu Shuang-Jiang Liu Christensenella minuta interacts with multiple gut bacteria Frontiers in Microbiology Christensenellaceae intestinal microorganism co-occurrence network Faecalibacterium prausnitzii Klebsiella pneumoniae nutrient cross-feeding |
| title | Christensenella minuta interacts with multiple gut bacteria |
| title_full | Christensenella minuta interacts with multiple gut bacteria |
| title_fullStr | Christensenella minuta interacts with multiple gut bacteria |
| title_full_unstemmed | Christensenella minuta interacts with multiple gut bacteria |
| title_short | Christensenella minuta interacts with multiple gut bacteria |
| title_sort | christensenella minuta interacts with multiple gut bacteria |
| topic | Christensenellaceae intestinal microorganism co-occurrence network Faecalibacterium prausnitzii Klebsiella pneumoniae nutrient cross-feeding |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2024.1301073/full |
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