Hypoxia stimulates the migration and invasion of osteosarcoma via up-regulating the NUSAP1 expression
Osteosarcoma is a highly aggressive malignant tumor, which most commonly occurs in children and adolescents. This study aims to reveal that hypoxia promotes the invasion of osteosarcoma cells by up-regulating the expression of NUSAP1. The expression of HIF-1α and NUSAP1 was significantly up-regulate...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
De Gruyter
2021-07-01
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| Series: | Open Medicine |
| Subjects: | |
| Online Access: | https://doi.org/10.1515/med-2020-0180 |
| _version_ | 1811202975921602560 |
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| author | Zhang Ling Song Jingtao Xin Xu Sun Donghong Huang Huiting Chen Yang Zhang Tao Zhang Yiming |
| author_facet | Zhang Ling Song Jingtao Xin Xu Sun Donghong Huang Huiting Chen Yang Zhang Tao Zhang Yiming |
| author_sort | Zhang Ling |
| collection | DOAJ |
| description | Osteosarcoma is a highly aggressive malignant tumor, which most commonly occurs in children and adolescents. This study aims to reveal that hypoxia promotes the invasion of osteosarcoma cells by up-regulating the expression of NUSAP1. The expression of HIF-1α and NUSAP1 was significantly up-regulated in MG63 cells cultured in hypoxia for 6–36 h. Furthermore, hypoxia induced the migration and invasion of MG63 cells and regulated the level of E-cad, N-cad, Vimentin, Snail, Slug, MMP2, and MMP9 proteins. Importantly, knockdown of NUSAP1 inhibited hypoxia-induced cell migration and invasion. In the hypoxia microenvironment, the addition of HIF-1α inhibitor or the transfection of siRNA specifically targeting HIF-1α significantly reduced the expression of HIF-1α and NUSAP1 and markedly inhibited the migration and invasion of MG63 cells under the hypoxia microenvironment. In conclusion, hypoxia induced the expression of NUSAP1 in a HIF-1α-dependent manner, stimulating the migration and invasion of MG63 cells. |
| first_indexed | 2024-04-12T02:46:59Z |
| format | Article |
| id | doaj.art-e42794eaa9c94b47bb3a69870d22f8d6 |
| institution | Directory Open Access Journal |
| issn | 2391-5463 |
| language | English |
| last_indexed | 2024-04-12T02:46:59Z |
| publishDate | 2021-07-01 |
| publisher | De Gruyter |
| record_format | Article |
| series | Open Medicine |
| spelling | doaj.art-e42794eaa9c94b47bb3a69870d22f8d62022-12-22T03:51:08ZengDe GruyterOpen Medicine2391-54632021-07-011611083108910.1515/med-2020-0180Hypoxia stimulates the migration and invasion of osteosarcoma via up-regulating the NUSAP1 expressionZhang Ling0Song Jingtao1Xin Xu2Sun Donghong3Huang Huiting4Chen Yang5Zhang Tao6Zhang Yiming7Department of Orthopedics, Huabei Petroleum General Hospital, Huizhan Road, Renqiu 062552, Hebei, ChinaDepartment of Orthopedics, Huabei Petroleum General Hospital, Huizhan Road, Renqiu 062552, Hebei, ChinaDepartment of Orthopedics, Huabei Petroleum General Hospital, Huizhan Road, Renqiu 062552, Hebei, ChinaDepartment of Orthopedics, Huabei Petroleum General Hospital, Huizhan Road, Renqiu 062552, Hebei, ChinaDepartment of Orthopedics, Huabei Petroleum General Hospital, Huizhan Road, Renqiu 062552, Hebei, ChinaDepartment of Orthopedics, Huabei Petroleum General Hospital, Huizhan Road, Renqiu 062552, Hebei, ChinaDepartment of Orthopedics, Tianjin Beichen District Chinese Medicine Hospital, Tianjin 300400, ChinaDepartment of Clinical Medicine, Tianjin Medical University, Tianjin 300070, ChinaOsteosarcoma is a highly aggressive malignant tumor, which most commonly occurs in children and adolescents. This study aims to reveal that hypoxia promotes the invasion of osteosarcoma cells by up-regulating the expression of NUSAP1. The expression of HIF-1α and NUSAP1 was significantly up-regulated in MG63 cells cultured in hypoxia for 6–36 h. Furthermore, hypoxia induced the migration and invasion of MG63 cells and regulated the level of E-cad, N-cad, Vimentin, Snail, Slug, MMP2, and MMP9 proteins. Importantly, knockdown of NUSAP1 inhibited hypoxia-induced cell migration and invasion. In the hypoxia microenvironment, the addition of HIF-1α inhibitor or the transfection of siRNA specifically targeting HIF-1α significantly reduced the expression of HIF-1α and NUSAP1 and markedly inhibited the migration and invasion of MG63 cells under the hypoxia microenvironment. In conclusion, hypoxia induced the expression of NUSAP1 in a HIF-1α-dependent manner, stimulating the migration and invasion of MG63 cells.https://doi.org/10.1515/med-2020-0180osteosarcomahypoxiahif-1αnusap1migrationinvasion |
| spellingShingle | Zhang Ling Song Jingtao Xin Xu Sun Donghong Huang Huiting Chen Yang Zhang Tao Zhang Yiming Hypoxia stimulates the migration and invasion of osteosarcoma via up-regulating the NUSAP1 expression Open Medicine osteosarcoma hypoxia hif-1α nusap1 migration invasion |
| title | Hypoxia stimulates the migration and invasion of osteosarcoma via up-regulating the NUSAP1 expression |
| title_full | Hypoxia stimulates the migration and invasion of osteosarcoma via up-regulating the NUSAP1 expression |
| title_fullStr | Hypoxia stimulates the migration and invasion of osteosarcoma via up-regulating the NUSAP1 expression |
| title_full_unstemmed | Hypoxia stimulates the migration and invasion of osteosarcoma via up-regulating the NUSAP1 expression |
| title_short | Hypoxia stimulates the migration and invasion of osteosarcoma via up-regulating the NUSAP1 expression |
| title_sort | hypoxia stimulates the migration and invasion of osteosarcoma via up regulating the nusap1 expression |
| topic | osteosarcoma hypoxia hif-1α nusap1 migration invasion |
| url | https://doi.org/10.1515/med-2020-0180 |
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