Overexpression of TRIM44 is related to invasive potential and malignant outcomes in esophageal squamous cell carcinoma
Recent studies have shown that some members of the tripartite motif-containing protein family function as important regulators for carcinogenesis. In this study, we investigated whether tripartite motif-containing protein 44 acts as a cancer-promoting gene through its overexpression in esophageal sq...
| Main Authors: | , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
IOS Press
2017-05-01
|
| Series: | Tumor Biology |
| Online Access: | https://doi.org/10.1177/1010428317700409 |
| _version_ | 1818948275690012672 |
|---|---|
| author | Tsutomu Kawaguchi Shuhei Komatsu Daisuke Ichikawa Shoji Hirajima Yukihisa Nishimura Hirotaka Konishi Atsushi Shiozaki Hitoshi Fujiwara Kazuma Okamoto Hitoshi Tsuda Eigo Otsuji |
| author_facet | Tsutomu Kawaguchi Shuhei Komatsu Daisuke Ichikawa Shoji Hirajima Yukihisa Nishimura Hirotaka Konishi Atsushi Shiozaki Hitoshi Fujiwara Kazuma Okamoto Hitoshi Tsuda Eigo Otsuji |
| author_sort | Tsutomu Kawaguchi |
| collection | DOAJ |
| description | Recent studies have shown that some members of the tripartite motif-containing protein family function as important regulators for carcinogenesis. In this study, we investigated whether tripartite motif-containing protein 44 acts as a cancer-promoting gene through its overexpression in esophageal squamous cell carcinoma. We analyzed esophageal squamous cell carcinoma cell lines to evaluate malignant potential and also analyzed 68 primary tumors to evaluate clinical relevance of tripartite motif-containing protein 44 protein in esophageal squamous cell carcinoma patients. Expression of the tripartite motif-containing protein 44 protein was detected in esophageal squamous cell carcinoma cell lines (8/14 cell lines; 57%) and primary tumor samples of esophageal squamous cell carcinoma (39/68 cases; 57%). Knockdown of tripartite motif-containing protein 44 expression in esophageal squamous cell carcinoma cells using several specific small interfering RNAs inhibited cell migration and invasion, but not cell proliferation. Immunohistochemical analysis demonstrated that the overexpression of the tripartite motif-containing protein 44 protein in the tumor infiltrated region was associated with the status of lymph node metastasis ( p = 0.049), and the overall survival rates were significantly worse among patients with tripartite motif-containing protein 44–overexpressing tumors than those with non-expressing tumors ( p = 0.029). Moreover, multivariate Cox regression model identified that overexpression of the tripartite motif-containing protein 44 protein was an independent worse prognostic factor (hazard ratio = 2.815; p = 0.041), as well as lymphatic invasion (hazard ratio = 2.735; p = 0.037). These results suggest that tripartite motif-containing protein 44 protein could play a crucial role in tumor invasion through its overexpression and highlight its usefulness as a predictor and potential therapeutic target in esophageal squamous cell carcinoma. |
| first_indexed | 2024-12-20T08:44:13Z |
| format | Article |
| id | doaj.art-e42d0e88bb75497c80e09b858ea46596 |
| institution | Directory Open Access Journal |
| issn | 1423-0380 |
| language | English |
| last_indexed | 2024-12-20T08:44:13Z |
| publishDate | 2017-05-01 |
| publisher | IOS Press |
| record_format | Article |
| series | Tumor Biology |
| spelling | doaj.art-e42d0e88bb75497c80e09b858ea465962022-12-21T19:46:18ZengIOS PressTumor Biology1423-03802017-05-013910.1177/1010428317700409Overexpression of TRIM44 is related to invasive potential and malignant outcomes in esophageal squamous cell carcinomaTsutomu Kawaguchi0Shuhei Komatsu1Daisuke Ichikawa2Shoji Hirajima3Yukihisa Nishimura4Hirotaka Konishi5Atsushi Shiozaki6Hitoshi Fujiwara7Kazuma Okamoto8Hitoshi Tsuda9Eigo Otsuji10Division of Digestive Surgery, Department of Surgery, Kyoto Prefectural University of Medicine, Kyoto, JapanDivision of Digestive Surgery, Department of Surgery, Kyoto Prefectural University of Medicine, Kyoto, JapanDivision of Digestive Surgery, Department of Surgery, Kyoto Prefectural University of Medicine, Kyoto, JapanDivision of Digestive Surgery, Department of Surgery, Kyoto Prefectural University of Medicine, Kyoto, JapanDivision of Digestive Surgery, Department of Surgery, Kyoto Prefectural University of Medicine, Kyoto, JapanDivision of Digestive Surgery, Department of Surgery, Kyoto Prefectural University of Medicine, Kyoto, JapanDivision of Digestive Surgery, Department of Surgery, Kyoto Prefectural University of Medicine, Kyoto, JapanDivision of Digestive Surgery, Department of Surgery, Kyoto Prefectural University of Medicine, Kyoto, JapanDivision of Digestive Surgery, Department of Surgery, Kyoto Prefectural University of Medicine, Kyoto, JapanDepartment of Basic Pathology, National Defense Medical College, Saitama, JapanDivision of Digestive Surgery, Department of Surgery, Kyoto Prefectural University of Medicine, Kyoto, JapanRecent studies have shown that some members of the tripartite motif-containing protein family function as important regulators for carcinogenesis. In this study, we investigated whether tripartite motif-containing protein 44 acts as a cancer-promoting gene through its overexpression in esophageal squamous cell carcinoma. We analyzed esophageal squamous cell carcinoma cell lines to evaluate malignant potential and also analyzed 68 primary tumors to evaluate clinical relevance of tripartite motif-containing protein 44 protein in esophageal squamous cell carcinoma patients. Expression of the tripartite motif-containing protein 44 protein was detected in esophageal squamous cell carcinoma cell lines (8/14 cell lines; 57%) and primary tumor samples of esophageal squamous cell carcinoma (39/68 cases; 57%). Knockdown of tripartite motif-containing protein 44 expression in esophageal squamous cell carcinoma cells using several specific small interfering RNAs inhibited cell migration and invasion, but not cell proliferation. Immunohistochemical analysis demonstrated that the overexpression of the tripartite motif-containing protein 44 protein in the tumor infiltrated region was associated with the status of lymph node metastasis ( p = 0.049), and the overall survival rates were significantly worse among patients with tripartite motif-containing protein 44–overexpressing tumors than those with non-expressing tumors ( p = 0.029). Moreover, multivariate Cox regression model identified that overexpression of the tripartite motif-containing protein 44 protein was an independent worse prognostic factor (hazard ratio = 2.815; p = 0.041), as well as lymphatic invasion (hazard ratio = 2.735; p = 0.037). These results suggest that tripartite motif-containing protein 44 protein could play a crucial role in tumor invasion through its overexpression and highlight its usefulness as a predictor and potential therapeutic target in esophageal squamous cell carcinoma.https://doi.org/10.1177/1010428317700409 |
| spellingShingle | Tsutomu Kawaguchi Shuhei Komatsu Daisuke Ichikawa Shoji Hirajima Yukihisa Nishimura Hirotaka Konishi Atsushi Shiozaki Hitoshi Fujiwara Kazuma Okamoto Hitoshi Tsuda Eigo Otsuji Overexpression of TRIM44 is related to invasive potential and malignant outcomes in esophageal squamous cell carcinoma Tumor Biology |
| title | Overexpression of TRIM44 is related to invasive potential and malignant outcomes in esophageal squamous cell carcinoma |
| title_full | Overexpression of TRIM44 is related to invasive potential and malignant outcomes in esophageal squamous cell carcinoma |
| title_fullStr | Overexpression of TRIM44 is related to invasive potential and malignant outcomes in esophageal squamous cell carcinoma |
| title_full_unstemmed | Overexpression of TRIM44 is related to invasive potential and malignant outcomes in esophageal squamous cell carcinoma |
| title_short | Overexpression of TRIM44 is related to invasive potential and malignant outcomes in esophageal squamous cell carcinoma |
| title_sort | overexpression of trim44 is related to invasive potential and malignant outcomes in esophageal squamous cell carcinoma |
| url | https://doi.org/10.1177/1010428317700409 |
| work_keys_str_mv | AT tsutomukawaguchi overexpressionoftrim44isrelatedtoinvasivepotentialandmalignantoutcomesinesophagealsquamouscellcarcinoma AT shuheikomatsu overexpressionoftrim44isrelatedtoinvasivepotentialandmalignantoutcomesinesophagealsquamouscellcarcinoma AT daisukeichikawa overexpressionoftrim44isrelatedtoinvasivepotentialandmalignantoutcomesinesophagealsquamouscellcarcinoma AT shojihirajima overexpressionoftrim44isrelatedtoinvasivepotentialandmalignantoutcomesinesophagealsquamouscellcarcinoma AT yukihisanishimura overexpressionoftrim44isrelatedtoinvasivepotentialandmalignantoutcomesinesophagealsquamouscellcarcinoma AT hirotakakonishi overexpressionoftrim44isrelatedtoinvasivepotentialandmalignantoutcomesinesophagealsquamouscellcarcinoma AT atsushishiozaki overexpressionoftrim44isrelatedtoinvasivepotentialandmalignantoutcomesinesophagealsquamouscellcarcinoma AT hitoshifujiwara overexpressionoftrim44isrelatedtoinvasivepotentialandmalignantoutcomesinesophagealsquamouscellcarcinoma AT kazumaokamoto overexpressionoftrim44isrelatedtoinvasivepotentialandmalignantoutcomesinesophagealsquamouscellcarcinoma AT hitoshitsuda overexpressionoftrim44isrelatedtoinvasivepotentialandmalignantoutcomesinesophagealsquamouscellcarcinoma AT eigootsuji overexpressionoftrim44isrelatedtoinvasivepotentialandmalignantoutcomesinesophagealsquamouscellcarcinoma |